UniProtKB - A1A5S1 (PRP6_RAT)
Pre-mRNA-processing factor 6
Prpf6
Functioni
Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.
By similarityGO - Molecular functioni
- androgen receptor binding Source: RGD
- identical protein binding Source: RGD
- ribonucleoprotein complex binding Source: RGD
- RNA binding Source: RGD
- transcription coactivator activity Source: RGD
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: RGD
- RNA localization Source: RGD
- spliceosomal tri-snRNP complex assembly Source: RGD
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-RNO-72163, mRNA Splicing - Major Pathway R-RNO-72165, mRNA Splicing - Minor Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-processing factor 6Alternative name(s): PRP6 homolog U5 snRNP-associated 102 kDa protein Short name: U5-102 kDa protein |
Gene namesi | Name:Prpf6 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1307103, Prpf6 |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
- Nucleus speckle By similarity
Note: Localized in splicing speckles.By similarity
Nucleus
- catalytic step 2 spliceosome Source: RGD
- nuclear speck Source: UniProtKB
- nucleus Source: RGD
- U2-type precatalytic spliceosome Source: UniProtKB
- U4/U6 x U5 tri-snRNP complex Source: RGD
- U5 snRNP Source: RGD
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413096 | 1 – 941 | Pre-mRNA-processing factor 6Add BLAST | 941 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 143 | PhosphoserineCombined sources | 1 | |
Modified residuei | 180 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 266 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 275 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 279 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | A1A5S1 |
PaxDbi | A1A5S1 |
PeptideAtlasi | A1A5S1 |
PTM databases
iPTMneti | A1A5S1 |
PhosphoSitePlusi | A1A5S1 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000051498, Expressed in skeletal muscle tissue and 21 other tissues |
Genevisiblei | A1A5S1, RN |
Interactioni
Subunit structurei
Identified in the spliceosome B complex. Identified in the spliceosome C complex. Associates with the U5 snRNP particle.
Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, LSm proteins LSm2-8 and Sm proteins.
Interacts with ARAF1.
Interacts with AR and NR3C1, but not ESR1, independently of the presence of hormones.
By similarityGO - Molecular functioni
- androgen receptor binding Source: RGD
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 265839, 2 interactors |
STRINGi | 10116.ENSRNOP00000021723 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 384 – 416 | HAT 1Add BLAST | 33 | |
Repeati | 418 – 444 | HAT 2Add BLAST | 27 | |
Repeati | 445 – 476 | HAT 3Add BLAST | 32 | |
Repeati | 554 – 586 | HAT 4Add BLAST | 33 | |
Repeati | 588 – 620 | HAT 5Add BLAST | 33 | |
Repeati | 622 – 654 | HAT 6Add BLAST | 33 | |
Repeati | 689 – 721 | HAT 7Add BLAST | 33 | |
Repeati | 723 – 755 | HAT 8Add BLAST | 33 | |
Repeati | 855 – 887 | HAT 9Add BLAST | 33 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 79 | DisorderedSequence analysisAdd BLAST | 79 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 38 – 68 | Basic and acidic residuesSequence analysisAdd BLAST | 31 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0495, Eukaryota |
GeneTreei | ENSGT00550000075016 |
HOGENOMi | CLU_007010_0_0_1 |
InParanoidi | A1A5S1 |
OMAi | DGWAWYY |
OrthoDBi | 335779at2759 |
PhylomeDBi | A1A5S1 |
TreeFami | TF105743 |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
InterProi | View protein in InterPro IPR003107, HAT IPR010491, PRP1_N IPR027108, Prp6/Prp1/STA1 IPR045075, Syf1-like IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
PANTHERi | PTHR11246, PTHR11246, 1 hit PTHR11246:SF1, PTHR11246:SF1, 1 hit |
Pfami | View protein in Pfam PF06424, PRP1_N, 1 hit |
SMARTi | View protein in SMART SM00386, HAT, 13 hits SM00028, TPR, 4 hits |
SUPFAMi | SSF48452, SSF48452, 4 hits |
PROSITEi | View protein in PROSITE PS50293, TPR_REGION, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP
60 70 80 90 100
PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE
110 120 130 140 150
ADAIYAALDK RMDERRKERR EQREKEEIEK YRMERPKIQQ QFSDLKRKLA
160 170 180 190 200
EVTEEEWLSI PEVGDARNKR QRNPRYEKLT PVPDSFFAKH LQTGENHTSV
210 220 230 240 250
DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM RKIGQARNTL
260 270 280 290 300
MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS
310 320 330 340 350
VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA
360 370 380 390 400
RLQPGDTAKA VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV
410 420 430 440 450
PNSVRLWKAA VELEEPEDAR IMLSRAVECC PTSVELWLAL ARLETYENAR
460 470 480 490 500
KVLNKARENI PTDRHIWITA AKLEEANGNT QMVEKIIDRA ITSLRANGVE
510 520 530 540 550
INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK HTWMEDADSC
560 570 580 590 600
VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA
610 620 630 640 650
VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK
660 670 680 690 700
LESENNEYER ARRLLAKARS SAPTARVFMK SVKLEWVLGN ITAAQELCEE
710 720 730 740 750
ALRHYEDFPK LWMMKGQIEE QGELMERARE AYNQGLKKCP HSTPLWLLLS
760 770 780 790 800
RLEEKIGQLT RARAILEKSR LKNPKNPGLW LESVRLEYRA GLKNIANTLM
810 820 830 840 850
AKALQECPNS GILWSEAVFL EARPQRKTKS VDALKKCEHD PHVLLAVAKL
860 870 880 890 900
FWSERKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR
910 920 930 940
CENAEPRHGE LWCAVSKDIT NWQRKIGEIL VLVAARIKNT F
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3ZCA7 | D3ZCA7_RAT | Long intergenic non-protein coding ... | Linc00176 RGD1561282, RGD1561282_predicted, rCG_63166 | 188 | Annotation score: | ||
A0A8I6AVE4 | A0A8I6AVE4_RAT | Long intergenic non-protein coding ... | Linc00176 | 947 | Annotation score: | ||
A0A8I6AW80 | A0A8I6AW80_RAT | Long intergenic non-protein coding ... | Linc00176 | 1,004 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474066 Genomic DNA Translation: EDL88695.1 BC128779 mRNA Translation: AAI28780.1 |
RefSeqi | NP_001073234.1, NM_001079766.1 |
Genome annotation databases
Ensembli | ENSRNOT00000083423; ENSRNOP00000073536; ENSRNOG00000051498 |
GeneIDi | 366276 |
KEGGi | rno:366276 |
UCSCi | RGD:1307103, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474066 Genomic DNA Translation: EDL88695.1 BC128779 mRNA Translation: AAI28780.1 |
RefSeqi | NP_001073234.1, NM_001079766.1 |
3D structure databases
AlphaFoldDBi | A1A5S1 |
SMRi | A1A5S1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 265839, 2 interactors |
STRINGi | 10116.ENSRNOP00000021723 |
PTM databases
iPTMneti | A1A5S1 |
PhosphoSitePlusi | A1A5S1 |
Proteomic databases
jPOSTi | A1A5S1 |
PaxDbi | A1A5S1 |
PeptideAtlasi | A1A5S1 |
Genome annotation databases
Ensembli | ENSRNOT00000083423; ENSRNOP00000073536; ENSRNOG00000051498 |
GeneIDi | 366276 |
KEGGi | rno:366276 |
UCSCi | RGD:1307103, rat |
Organism-specific databases
CTDi | 24148 |
RGDi | 1307103, Prpf6 |
Phylogenomic databases
eggNOGi | KOG0495, Eukaryota |
GeneTreei | ENSGT00550000075016 |
HOGENOMi | CLU_007010_0_0_1 |
InParanoidi | A1A5S1 |
OMAi | DGWAWYY |
OrthoDBi | 335779at2759 |
PhylomeDBi | A1A5S1 |
TreeFami | TF105743 |
Enzyme and pathway databases
Reactomei | R-RNO-72163, mRNA Splicing - Major Pathway R-RNO-72165, mRNA Splicing - Minor Pathway |
Miscellaneous databases
PROi | PR:A1A5S1 |
Gene expression databases
Bgeei | ENSRNOG00000051498, Expressed in skeletal muscle tissue and 21 other tissues |
Genevisiblei | A1A5S1, RN |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
InterProi | View protein in InterPro IPR003107, HAT IPR010491, PRP1_N IPR027108, Prp6/Prp1/STA1 IPR045075, Syf1-like IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
PANTHERi | PTHR11246, PTHR11246, 1 hit PTHR11246:SF1, PTHR11246:SF1, 1 hit |
Pfami | View protein in Pfam PF06424, PRP1_N, 1 hit |
SMARTi | View protein in SMART SM00386, HAT, 13 hits SM00028, TPR, 4 hits |
SUPFAMi | SSF48452, SSF48452, 4 hits |
PROSITEi | View protein in PROSITE PS50293, TPR_REGION, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP6_RAT | |
Accessioni | A1A5S1Primary (citable) accession number: A1A5S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |