UniProtKB - A0T2N3 (APJB_DANRE)
Protein
Apelin receptor B
Gene
aplnrb
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Receptor for apelin receptor early endogenous ligand (apela) and apelin (apln) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed:17336905, PubMed:17336906, PubMed:24316148, PubMed:24407481). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for apela hormone, promoting endoderm and mesendoderm cell migration and regulating the migration of cells fated to become myocardial progenitors, respectively (PubMed:17336905, PubMed:17336906, PubMed:24316148, PubMed:24407481, PubMed:26017639). Positively regulates angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis (PubMed:26017639). May promote sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development (By similarity). Required for cardiovascular development, particularly for intersomitic vein angiogenesis by acting as a receptor for apln hormone (By similarity). Plays a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (By similarity). Acts redundantly with agtrl1a in heart development (PubMed:17336906).By similarity5 Publications
GO - Molecular functioni
- apelin receptor activity Source: ZFIN
- G protein-coupled receptor activity Source: GO_Central
GO - Biological processi
- angioblast cell migration from lateral mesoderm to midline Source: ZFIN
- angiogenesis Source: UniProtKB-KW
- blood vessel development Source: GO_Central
- cardiac muscle tissue development Source: UniProtKB
- cell migration Source: UniProtKB
- coronary vasculature development Source: UniProtKB
- endodermal cell differentiation Source: ZFIN
- establishment of epithelial cell polarity Source: ZFIN
- gastrulation Source: ZFIN
- gastrulation with mouth forming second Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: GO_Central
- heart development Source: UniProtKB
- mesodermal cell migration Source: ZFIN
- negative regulation of cAMP-mediated signaling Source: UniProtKB
- positive regulation of angiogenesis Source: UniProtKB
- positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: UniProtKB
- positive regulation of inhibitory G protein-coupled receptor phosphorylation Source: UniProtKB
- positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
- regulation of nodal signaling pathway Source: ZFIN
- vasculogenesis Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, G-protein coupled receptor, Receptor, Transducer |
Biological process | Angiogenesis, Gastrulation |
Names & Taxonomyi
Protein namesi | Recommended name: Apelin receptor BAlternative name(s): Angiotensin II receptor-like 1b Angiotensin receptor-like 1b G-protein coupled receptor APJ B Protein grinch |
Gene namesi | Name:aplnrb Synonyms:agtrl1b, grn1 Publication ORF Names:zgc:114063 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-050913-90, aplnrb |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Internalized to the cytoplasm after exposure to apelin (apln). After exposure to apelin receptor early endogenous ligand (apela), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane.By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 36 | ExtracellularSequence analysisAdd BLAST | 36 | |
Transmembranei | 37 – 57 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 58 – 75 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 76 – 96 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 97 – 108 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 109 – 129 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 130 – 151 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 152 – 172 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 173 – 213 | ExtracellularSequence analysisAdd BLAST | 41 | |
Transmembranei | 214 – 234 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 235 – 251 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 252 – 272 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 273 – 286 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 287 – 307 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 308 – 359 | CytoplasmicSequence analysisAdd BLAST | 52 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Morpholino knockdown of the protein induces embryonic lethality due to cardiac dysplasia with little to no blood circulation around 6 days post-fertilization (dpf) (PubMed:24316148). Mutant embryos show pericardial edema and accumulation of erythrocytes in the intermediate cell mass (ICM) at 30 hours post-fertilization (hpf) (PubMed:24316148). Display decreased angioblast migration to the embryonic midline during late gastrulation (PubMed:24316148, PubMed:24407481, PubMed:26017639).3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 | W → L in grns608; defective in early myocardial specification. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000312594 | 1 – 359 | Apelin receptor BAdd BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 6 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 21 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 182 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | A0T2N3 |
Expressioni
Tissue specificityi
Mesendodermal expression at the blastoderm margin appears by 4.5 hpf. At early gastrulation, expression is maintained ventrolaterally while expression in dorsal cells and random deep cells declines. During gastrulation and segmentation, expression is maintained in adaxial, intermediate, and lateral plate mesoderm. During late segmentation, expressed in several regions including the forming heart. By 24 hpf, expressed in the dorsal aorta, caudal vein, and intersomitic blood vessels.1 Publication
Gene expression databases
Bgeei | ENSDARG00000036670, Expressed in spleen and 39 other tissues |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222298 |
HOGENOMi | CLU_009579_8_1_1 |
InParanoidi | A0T2N3 |
OMAi | FTVWRAQ |
OrthoDBi | 788659at2759 |
PhylomeDBi | A0T2N3 |
TreeFami | TF330024 |
Family and domain databases
InterProi | View protein in InterPro IPR000248, ATII_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00241, ANGIOTENSINR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
A0T2N3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNAMDNMTAD YSPDYFDDAV NSSMCEYDEW EPSYSLIPVL YMLIFILGLT
60 70 80 90 100
GNGVVIFTVW RAQSKRRAAD VYIGNLALAD LTFVVTLPLW AVYTALGYHW
110 120 130 140 150
PFGVALCKIS SYVVLLNMYA SVFCLTCLSL DRYMAIVHSL TSTQLRTRGH
160 170 180 190 200
MRASLTAIWL LSGVLAAPTL LFRTTVYDVE TNRTSCAMDF NLVVSQPGQE
210 220 230 240 250
TYWIAGLSIS STALGFLIPL LAMMVCYGFI GCTVTRHFNS LRKEDQRKRR
260 270 280 290 300
LLKIITTLVV VFAACWMPFH VVKTMDALSY LNLAPDSCTF LNLLLLAHPY
310 320 330 340 350
ATCLAYVNSC LNPLLYAFFD LRFRSQCLCL LNLKKALHAS PASSLSSQKT
EAQSLATKV
Sequence cautioni
The sequence AAH97125 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF079888 mRNA Translation: ABK63803.1 BC097125 mRNA Translation: AAH97125.1 Different initiation. |
RefSeqi | NP_001025368.2, NM_001030197.2 |
Genome annotation databases
Ensembli | ENSDART00000129643; ENSDARP00000108783; ENSDARG00000036670 |
GeneIDi | 565492 |
KEGGi | dre:565492 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF079888 mRNA Translation: ABK63803.1 BC097125 mRNA Translation: AAH97125.1 Different initiation. |
RefSeqi | NP_001025368.2, NM_001030197.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000108783 |
Protein family/group databases
GPCRDBi | Search... |
Proteomic databases
PaxDbi | A0T2N3 |
Genome annotation databases
Ensembli | ENSDART00000129643; ENSDARP00000108783; ENSDARG00000036670 |
GeneIDi | 565492 |
KEGGi | dre:565492 |
Organism-specific databases
CTDi | 565492 |
ZFINi | ZDB-GENE-050913-90, aplnrb |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222298 |
HOGENOMi | CLU_009579_8_1_1 |
InParanoidi | A0T2N3 |
OMAi | FTVWRAQ |
OrthoDBi | 788659at2759 |
PhylomeDBi | A0T2N3 |
TreeFami | TF330024 |
Miscellaneous databases
PROi | PR:A0T2N3 |
Gene expression databases
Bgeei | ENSDARG00000036670, Expressed in spleen and 39 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000248, ATII_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00241, ANGIOTENSINR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | APJB_DANRE | |
Accessioni | A0T2N3Primary (citable) accession number: A0T2N3 Secondary accession number(s): Q4V905 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | January 9, 2007 | |
Last modified: | December 2, 2020 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries