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Protein

Irditoxin subunit A

Gene
N/A
Organism
Boiga irregularis (Brown tree snake)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This bird and reptile-specific postsynaptic neurotoxin inhibits the chick muscle alpha-1/beta/gamma/delta (CHRNA1/CHRNB1/CHRNG/CHNRD) -nicotinic acetylcholine receptor (nAChR) 100-fold more compared with the mouse receptor. In vivo, produces rapid flaccid paralysis, dyspnea and increased respiratory rate in geckos. At sublethal doses geckos were immobilized for up to three days and then recovered. Chicks injected with lethal doses showed rapid onset of inactivity, dyspnea and neck droop, and no extended paralysis with survival was seen.1 Publication

Miscellaneous

IC50 is 11.2 nM for indirectly stimulated, nerve-evoked twitch responses of the chick biventer cervicis muscle acetylcholine receptor channel.1 Publication
Is not toxic to mice at doses up to 25 µg/g (i.p.). Does not inhibit mouse alpha-3/beta-2- and alpha-7-nicotinic acetylcholine receptors.1 Publication

GO - Molecular functioni

  • acetylcholine receptor inhibitor activity Source: UniProtKB
  • toxin activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Irditoxin subunit A
Short name:
IrTxA
OrganismiBoiga irregularis (Brown tree snake)
Taxonomic identifieri92519 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaColubridaeColubrinaeBoiga

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.55 mg/kg by intraperitoneal injection into geckos, and 0.22 mg/kg by intraperitoneal injection into chicks.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000031378820 – 341 PublicationAdd BLAST15
ChainiPRO_500017133435 – 109Irditoxin subunit AAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi44 ↔ 661 Publication
Disulfide bondi47 ↔ 551 Publication
Disulfide bondi61 ↔ 851 Publication
Disulfide bondi76Interchain (with C-52 in Irditoxin subunit B)1 Publication
Disulfide bondi89 ↔ 1001 Publication
Disulfide bondi101 ↔ 1061 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Subunit structurei

Heterodimer of A and B chains; disulfide-linked.1 Publication

Structurei

Secondary structure

1109
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliA0S864
SMRiA0S864
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA0S864

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553

Family and domain databases

InterProiView protein in InterPro
IPR018354 Snake_toxin_con_site
IPR035076 Toxin/TOLIP
PfamiView protein in Pfam
PF00087 Toxin_TOLIP, 1 hit
PROSITEiView protein in PROSITE
PS00272 SNAKE_TOXIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0S864-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTLLLAVAV VAFVCLGSAD QLGLGRQQID WGQGQAVGPP YTLCFECNRM
60 70 80 90 100
TSSDCSTALR CYRGSCYTLY RPDENCELKW AVKGCAETCP TAGPNERVKC

CRSPRCNDD
Length:109
Mass (Da):11,938
Last modified:January 9, 2007 - v1
Checksum:iD2E1E5D42E467A12
GO

Mass spectrometryi

Molecular mass is 8379.56±0.20 Da from positions 35 - 109. Determined by ESI. The measured mass is that of the reduced peptide.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ304538 mRNA Translation: ABC17853.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ304538 mRNA Translation: ABC17853.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H7ZX-ray1.50A35-109[»]
ProteinModelPortaliA0S864
SMRiA0S864
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553

Miscellaneous databases

EvolutionaryTraceiA0S864

Family and domain databases

InterProiView protein in InterPro
IPR018354 Snake_toxin_con_site
IPR035076 Toxin/TOLIP
PfamiView protein in Pfam
PF00087 Toxin_TOLIP, 1 hit
PROSITEiView protein in PROSITE
PS00272 SNAKE_TOXIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3NBA_BOIIR
AccessioniPrimary (citable) accession number: A0S864
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 22, 2017
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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