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UniProtKB - A0R667 (A0R667_MYCS2)
Protein
DNA polymerase IV
Gene
dinP
Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Functioni
Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.
UniRule annotationARBA annotationCatalytic activityi
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)UniRule annotationARBA annotationEC:2.7.7.7UniRule annotationARBA annotation
Cofactori
Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 15 | MagnesiumUniRule annotation | 1 | |
Sitei | 20 | Substrate discriminationUniRule annotation | 1 | |
Metal bindingi | 105 | MagnesiumUniRule annotation | 1 | |
Active sitei | 106 | UniRule annotation | 1 |
GO - Molecular functioni
- damaged DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA repair Source: UniProtKB-UniRule
- SOS response Source: UniProt
Keywordsi
Molecular function | DNA-bindingUniRule annotation, DNA-directed DNA polymeraseUniRule annotationARBA annotation, Mutator proteinUniRule annotationARBA annotation, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repairUniRule annotationARBA annotation, DNA replicationUniRule annotationARBA annotation |
Ligand | MagnesiumUniRule annotationARBA annotation, Metal-bindingUniRule annotationARBA annotation |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)Short name: Pol IVUniRule annotation |
Gene namesi | Name:dinPImported Synonyms:dinBUniRule annotation Ordered Locus Names:MSMEG_6443Imported |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)Imported |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationInteractioni
Subunit structurei
Monomer.
UniRule annotationARBA annotationProtein-protein interaction databases
STRINGi | 246196.MSMEI_6274 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 187 | UmuCInterPro annotationAdd BLAST | 177 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0389, Bacteria |
OMAi | VKKFHGV |
OrthoDBi | 442163at2 |
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
i Sequence
Sequence statusi: Complete.
A0R667-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFVSAAESAS ILHADLDSFY ASVEQRDDPA LRGRPVIVGG GVVLAASYEA
60 70 80 90 100
KAYGVRTAMS GGQARALCPQ AIVVPPRMAA YTQASRDVFA VFHDTTPLVE
110 120 130 140 150
PLSVDEAFLD VSGLARVSGT PVEIAARLRA RVREQVGLPI TVGIARTKFL
160 170 180 190 200
AKVASQEGKP DGLLLVPPDR ELAFLHPLPV RRLWGVGAKT AEKLRAHGIE
210 220 230 240 250
TVADVAELSE ATLGSMVGGA MGRQLFTLSR NIDRRRVTTG VRRSSVGAQR
260 270 280 290 300
ALGRRGNSMS AAEVDAVVVN LVDRITRRMR AAGRTGRTVV LRLRFDDFGR
310 320 330 340 350
ATRSHTMPWA TASTDVILCA ARELVAAAAP LIAERGLTLI GFAVSNIDRG
360 370 380 390 400
GTQQLELPFA EQPDPVAIDS AIDQVRQRFG NAVLTRGVLV GRDPGLEMPM
LPD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK74774.1 |
RefSeqi | WP_011731284.1, NZ_SIJM01000013.1 YP_890655.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK74774; ABK74774; MSMEG_6443 |
GeneIDi | 66737718 |
KEGGi | msm:MSMEG_6443 |
PATRICi | fig|246196.19.peg.6267 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK74774.1 |
RefSeqi | WP_011731284.1, NZ_SIJM01000013.1 YP_890655.1, NC_008596.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_6274 |
Genome annotation databases
EnsemblBacteriai | ABK74774; ABK74774; MSMEG_6443 |
GeneIDi | 66737718 |
KEGGi | msm:MSMEG_6443 |
PATRICi | fig|246196.19.peg.6267 |
Phylogenomic databases
eggNOGi | COG0389, Bacteria |
OMAi | VKKFHGV |
OrthoDBi | 442163at2 |
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A0R667_MYCS2 | |
Accessioni | A0R667Primary (citable) accession number: A0R667 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | January 9, 2007 |
Last sequence update: | January 9, 2007 | |
Last modified: | January 19, 2022 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |