UniProtKB - A0R665 (ETHA_MYCS2)
FAD-containing monooxygenase EthA
ethA
Functioni
Monooxygenase able to convert a wide range of ketones to the corresponding esters or lactones via a Baeyer-Villiger oxidation reaction. Can act on long-chain aliphatic ketones (2-hexanone to 2-dodecanone) and on aromatic ketones (phenylacetone and benzylacetone). Is also able to catalyze enantioselective sulfoxidation of methyl-p-tolylsulfide. In vivo, likely functions as a BVMO, but the exact nature of the physiological substrate(s) remains to be established.
By similarityIs responsible for the activation of several thiocarbamide-containing pro-drugs, such as ethionamide (ETH), isoxyl (ISO) and thiacetazone (TAC), into reactive species.
By similarityCatalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 15 | FADBy similarity | 1 | |
Binding sitei | 36 | FADBy similarity | 1 | |
Binding sitei | 56 | FADBy similarity | 1 | |
Binding sitei | 104 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Sitei | 292 | Transition state stabilizerBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 44 – 47 | FADBy similarity | 4 | |
Nucleotide bindingi | 54 – 56 | NADPBy similarity | 3 | |
Nucleotide bindingi | 183 – 189 | NADPBy similarity | 7 | |
Nucleotide bindingi | 207 – 208 | NADPBy similarity | 2 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: UniProtKB
- N,N-dimethylaniline monooxygenase activity Source: InterPro
- NADP binding Source: InterPro
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: FAD-containing monooxygenase EthA (EC:1.14.13.-By similarity)Alternative name(s): Baeyer-Villiger monooxygenase Short name: BVMO Prodrug activator EtaA |
Gene namesi | Name:ethA Ordered Locus Names:MSMEG_6440, MSMEI_6272 |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Note: Is most likely membrane-associated.By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000398583 | 1 – 492 | FAD-containing monooxygenase EthAAdd BLAST | 492 |
Proteomic databases
PRIDEi | A0R665 |
Expressioni
Inductioni
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2072, Bacteria |
OMAi | ASWTLKC |
OrthoDBi | 324612at2 |
Family and domain databases
Gene3Di | 3.50.50.60, 3 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR020946, Flavin_mOase-like |
Pfami | View protein in Pfam PF00743, FMO-like, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTEHFDVVIV GAGISGISTA WHLQDRCPTK SYVILERRAN IGGTWDLFKY
60 70 80 90 100
PGIRSDSDMF TLGFRFKPWT SAKSIADGPS IWNYINEAAQ ENGIDKHIRT
110 120 130 140 150
NHRVLGADWS DAENRWTITV EADGEQKQIT ASFLSVCSGY YNYDQGYSPE
160 170 180 190 200
FPGADDFAGQ IIHPQHWPED LDYAGKKIVV IGSGATAVTL IPSLVNGGAA
210 220 230 240 250
HVTMLQRSPT YIGSLPLVDP VAEKTNKYLP KNLAHFVNRW KAIAFSTAQY
260 270 280 290 300
QLARKFPNYM RKTLMTMAQR RLPEGFDVQK HFGPRYNPWD ERLCLAPNGD
310 320 330 340 350
LFKTIRAGKA DVVTDTIAKF TETGIKLTSG EELTADIIIT ATGLNMQLFG
360 370 380 390 400
GASLTRNGQE VDLTETMTYK GLMLSGVPNM AITFGYTNAS WTLKADLVSE
410 420 430 440 450
FICRVLNYMD DNGFDRVEPQ HPGDAVDALP FMDFNPGYFR RAMDSLPKSG
460 470 480 490
SRAPWRLKQN YFFDLRMIRY DKVDEESLHF TKHRAAVSAS SS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK75948.1 CP001663 Genomic DNA Translation: AFP42698.1 |
RefSeqi | WP_003897853.1, NZ_SIJM01000013.1 YP_890653.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK75948; ABK75948; MSMEG_6440 AFP42698; AFP42698; MSMEI_6272 |
GeneIDi | 66737716 |
KEGGi | msg:MSMEI_6272 msm:MSMEG_6440 |
PATRICi | fig|246196.19.peg.6265 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK75948.1 CP001663 Genomic DNA Translation: AFP42698.1 |
RefSeqi | WP_003897853.1, NZ_SIJM01000013.1 YP_890653.1, NC_008596.1 |
3D structure databases
AlphaFoldDBi | A0R665 |
SMRi | A0R665 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_6272 |
Proteomic databases
PRIDEi | A0R665 |
Genome annotation databases
EnsemblBacteriai | ABK75948; ABK75948; MSMEG_6440 AFP42698; AFP42698; MSMEI_6272 |
GeneIDi | 66737716 |
KEGGi | msg:MSMEI_6272 msm:MSMEG_6440 |
PATRICi | fig|246196.19.peg.6265 |
Phylogenomic databases
eggNOGi | COG2072, Bacteria |
OMAi | ASWTLKC |
OrthoDBi | 324612at2 |
Family and domain databases
Gene3Di | 3.50.50.60, 3 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR020946, Flavin_mOase-like |
Pfami | View protein in Pfam PF00743, FMO-like, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ETHA_MYCS2 | |
Accessioni | A0R665Primary (citable) accession number: A0R665 Secondary accession number(s): I7FUV7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | January 9, 2007 | |
Last modified: | May 25, 2022 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families