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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

rraA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateBy similarity1
Metal bindingi101Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandMetal-binding

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-6406-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Name:rraA
Ordered Locus Names:MSMEG_6439, MSMEI_6271
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_10000138492 – 159Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST158

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_6439

Structurei

3D structure databases

ProteinModelPortaliA0R664
SMRiA0R664
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 81Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX Bacteria
COG0684 LUCA
HOGENOMiHOG000252803
KOiK02553
OMAiRSCDTQF
OrthoDBiPOG091H03SM

Family and domain databases

CDDicd16841 RraA_family, 1 hit
InterProiView protein in InterPro
IPR010203 RraA
IPR005493 RraA/RraA-like
IPR036704 RraA/RraA-like_sf
PfamiView protein in Pfam
PF03737 RraA-like, 1 hit
SUPFAMiSSF89562 SSF89562, 1 hit
TIGRFAMsiTIGR01935 NOT-MenG, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0R664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEPRATAD LVDEIYPDVR SCDLQLQNYG GKIMFAGPVT TVRCFQDNAL
60 70 80 90 100
LKSILSTPGE GAVLVVDGAG SLHTALVGDV IAGLAADNGW SGVIVNGAVR
110 120 130 140 150
DAAALRTIDV GIKALGTNPR KGTKTGEGER DVEVSFGGVT FTPGDIAYCD

EDGIVVVTP
Length:159
Mass (Da):16,407
Last modified:January 9, 2007 - v1
Checksum:i108DAF5F15A38CD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK71829.1
CP001663 Genomic DNA Translation: AFP42697.1
RefSeqiWP_011731282.1, NZ_CP009494.1
YP_890652.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK71829; ABK71829; MSMEG_6439
AFP42697; AFP42697; MSMEI_6271
GeneIDi4535056
KEGGimsb:LJ00_31835
msg:MSMEI_6271
msm:MSMEG_6439
PATRICifig|246196.19.peg.6264

Similar proteinsi

Entry informationi

Entry nameiRRAAH_MYCS2
AccessioniPrimary (citable) accession number: A0R664
Secondary accession number(s): I7GAU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: June 20, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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