UniProtKB - A0R5T2 (LIGC1_MYCS2)
Protein
DNA ligase C1
Gene
ligC
Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Functioni
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair (By similarity). Has weak intrinsic nick joining activities and accumulates DNA-adenylate. Acts as a backup for LigD in the Ku-LigD-dependent NHEJ pathway.By similarity2 Publications
Catalytic activityi
- ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation EC:6.5.1.1
Cofactori
a divalent metal cationBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 32 | N6-AMP-lysine intermediateCurated | 1 |
GO - Molecular functioni
- ATP binding Source: InterPro
- DNA ligase (ATP) activity Source: UniProtKB-EC
GO - Biological processi
- DNA ligation involved in DNA repair Source: InterPro
- DNA recombination Source: InterPro
Keywordsi
Molecular function | Ligase |
Names & Taxonomyi
Protein namesi | Recommended name: DNA ligase C1 (EC:6.5.1.1PROSITE-ProRule annotation)Alternative name(s): Polydeoxyribonucleotide synthase [ATP] |
Gene namesi | Name:ligC Ordered Locus Names:MSMEG_6302, MSMEI_6137 |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Not essential; a double ligC1-ligC2 mutant grows normally, no effect on NHEJ. In a triple deletion with ligD NHEJ on blunt-ended plasmid is 90-fold impaired. In quadruple ligB-ligC1-ligC2-ligD deletions NHEJ on blunt and 5'-overhangs is 0.22 and 0.12% of wild-type respectively, only 4-fold decrease in 3'-overhang NHEJ.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000425951 | 1 – 348 | DNA ligase C1Add BLAST | 348 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent DNA ligase family.Curated
Phylogenomic databases
eggNOGi | COG1793, Bacteria |
OMAi | YRPHKSQ |
Family and domain databases
InterProi | View protein in InterPro IPR012309, DNA_ligase_ATP-dep_C IPR012310, DNA_ligase_ATP-dep_cent IPR016059, DNA_ligase_ATP-dep_CS IPR012340, NA-bd_OB-fold |
Pfami | View protein in Pfam PF04679, DNA_ligase_A_C, 1 hit PF01068, DNA_ligase_A_M, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS00697, DNA_LIGASE_A1, 1 hit |
i Sequence
Sequence statusi: Complete.
A0R5T2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLPVQPPIE PMLAKAQVKV PDEAGVWSYE PKWDGFRALV FRDGDDVVLQ
60 70 80 90 100
SRNGKDLGRY FPELLDALRD ELVEKCVLDG EVVVPRDIAG RVRLDWESLS
110 120 130 140 150
QRIHPAASRI KMLAEQTPAH FIGFDALALG DRSLLKEPFR VRREALAEAV
160 170 180 190 200
DNKRWCHVTR TSEDPALGTE WLKTFEGAGL DGVIAKRLDG PYLPGKREMV
210 220 230 240 250
KVKHHRDADC VAMGYRIHKS GDGIGSILLG LYRDDGELQM VGGAASFTAK
260 270 280 290 300
DRIKLLAELE PLREGDEMRE GDPSRWNSAA DKRWTPLRPE KVCEVAYDQM
310 320 330 340
EGNSVEGRRF RHAVKFLRWR PDREPSSCTF DQLDTPLNYD LYDVLEEQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK76125.1 CP001663 Genomic DNA Translation: AFP42567.1 |
RefSeqi | YP_890520.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK76125; ABK76125; MSMEG_6302 AFP42567; AFP42567; MSMEI_6137 |
KEGGi | msg:MSMEI_6137 msm:MSMEG_6302 |
PATRICi | fig|246196.19.peg.6138 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK76125.1 CP001663 Genomic DNA Translation: AFP42567.1 |
RefSeqi | YP_890520.1, NC_008596.1 |
3D structure databases
SMRi | A0R5T2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_6137 |
Genome annotation databases
EnsemblBacteriai | ABK76125; ABK76125; MSMEG_6302 AFP42567; AFP42567; MSMEI_6137 |
KEGGi | msg:MSMEI_6137 msm:MSMEG_6302 |
PATRICi | fig|246196.19.peg.6138 |
Phylogenomic databases
eggNOGi | COG1793, Bacteria |
OMAi | YRPHKSQ |
Family and domain databases
InterProi | View protein in InterPro IPR012309, DNA_ligase_ATP-dep_C IPR012310, DNA_ligase_ATP-dep_cent IPR016059, DNA_ligase_ATP-dep_CS IPR012340, NA-bd_OB-fold |
Pfami | View protein in Pfam PF04679, DNA_ligase_A_C, 1 hit PF01068, DNA_ligase_A_M, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS00697, DNA_LIGASE_A1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LIGC1_MYCS2 | |
Accessioni | A0R5T2Primary (citable) accession number: A0R5T2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 19, 2014 |
Last sequence update: | January 9, 2007 | |
Last modified: | October 7, 2020 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families