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Entry version 88 (11 Dec 2019)
Sequence version 1 (09 Jan 2007)
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Protein

Hercynine oxygenase

Gene

egtB

Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the oxidative sulfurization of hercynine (N-alpha,N-alpha,N-alpha-trimethyl-L-histidine) into hercynyl-gamma-L-glutamyl-L-cysteine sulfoxide, a step in the biosynthesis pathway of ergothioneine. Cannot use the alternative thiols cysteine, N-acetylcysteine, or glutathione instead of gamma-glutamylcysteine as substrates, and histidine is a poor sulfur acceptor substrate compared to hercynine.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Fe2+2 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 1.2 sec(-1).1 Publication
  1. KM=43 µM for hercynine1 Publication
  2. KM=78 µM for gamma-L-glutamyl-cysteine1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: ergothioneine biosynthesis

    This protein is involved in the pathway ergothioneine biosynthesis, which is part of Amino-acid biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway ergothioneine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi46Iron; via tele nitrogenBy similarity1
    Metal bindingi129Iron; via tele nitrogenBy similarity1
    Metal bindingi133Iron; via tele nitrogenBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei411Gamma-glutamylcysteineBy similarity1
    Binding sitei415Gamma-glutamylcysteineBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandIron, Metal-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    GCF_000015005:MSMEG_6249-MONOMER
    MetaCyc:MONOMER-17985
    MSME246196:G1H7P-6218-MONOMER

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA01014

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Hercynine oxygenaseUniRule annotation (EC:1.14.99.502 Publications)
    Alternative name(s):
    Gamma-glutamyl hercynylcysteine S-oxide synthaseUniRule annotation
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:egtB1 Publication
    Ordered Locus Names:MSMEG_6249, MSMEI_6088
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri246196 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000006158 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004136471 – 428Hercynine oxygenaseAdd BLAST428

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Monomer.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    246196.MSMEI_6088

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    A0R5N0

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni82 – 85Gamma-glutamylcysteine bindingBy similarity4

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the EgtB family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105E46 Bacteria
    COG1262 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000253478

    KEGG Orthology (KO)

    More...
    KOi
    K18912

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    CRGTFRN

    Database of Orthologous Groups

    More...
    OrthoDBi
    1377603at2

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.20.120.450, 1 hit
    3.90.1580.10, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_02035 EgtB, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR016187 CTDL_fold
    IPR024775 DinB-like
    IPR034660 DinB/YfiT-like
    IPR017806 EgtB
    IPR032890 EgtB_Actinobacteria
    IPR005532 SUMF_dom
    IPR042095 SUMF_sf

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF12867 DinB_2, 1 hit
    PF03781 FGE-sulfatase, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF109854 SSF109854, 1 hit
    SSF56436 SSF56436, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR03440 egtB_TIGR03440, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    A0R5N0-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MIARETLADE LALARERTLR LVEFDDAELH RQYNPLMSPL VWDLAHIGQQ
    60 70 80 90 100
    EELWLLRDGN PDRPGMLAPE VDRLYDAFEH SRASRVNLPL LPPSDARAYC
    110 120 130 140 150
    ATVRAKALDT LDTLPEDDPG FRFALVISHE NQHDETMLQA LNLREGPPLL
    160 170 180 190 200
    DTGIPLPAGR PGVAGTSVLV PGGPFVLGVD ALTEPHSLDN ERPAHVVDIP
    210 220 230 240 250
    SFRIGRVPVT NAEWREFIDD GGYDQPRWWS PRGWAHRQEA GLVAPQFWNP
    260 270 280 290 300
    DGTRTRFGHI EEIPGDEPVQ HVTFFEAEAY AAWAGARLPT EIEWEKACAW
    310 320 330 340 350
    DPVAGARRRF PWGSAQPSAA LANLGGDARR PAPVGAYPAG ASAYGAEQML
    360 370 380 390 400
    GDVWEWTSSP LRPWPGFTPM IYERYSTPFF EGTTSGDYRV LRGGSWAVAP
    410 420
    GILRPSFRNW DHPIRRQIFS GVRLAWDV
    Length:428
    Mass (Da):47,766
    Last modified:January 9, 2007 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i96ECEB89337CE85A
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK70251.1
    CP001663 Genomic DNA Translation: AFP42519.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_011731158.1, NZ_SIJM01000027.1
    YP_890468.1, NC_008596.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    ABK70251; ABK70251; MSMEG_6249
    AFP42519; AFP42519; MSMEI_6088

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    4533015

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    msb:LJ00_30900
    msg:MSMEI_6088
    msm:MSMEG_6249

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|246196.19.peg.6088

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK70251.1
    CP001663 Genomic DNA Translation: AFP42519.1
    RefSeqiWP_011731158.1, NZ_SIJM01000027.1
    YP_890468.1, NC_008596.1

    3D structure databases

    SMRiA0R5N0
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi246196.MSMEI_6088

    Genome annotation databases

    EnsemblBacteriaiABK70251; ABK70251; MSMEG_6249
    AFP42519; AFP42519; MSMEI_6088
    GeneIDi4533015
    KEGGimsb:LJ00_30900
    msg:MSMEI_6088
    msm:MSMEG_6249
    PATRICifig|246196.19.peg.6088

    Phylogenomic databases

    eggNOGiENOG4105E46 Bacteria
    COG1262 LUCA
    HOGENOMiHOG000253478
    KOiK18912
    OMAiCRGTFRN
    OrthoDBi1377603at2

    Enzyme and pathway databases

    UniPathwayiUPA01014
    BioCyciGCF_000015005:MSMEG_6249-MONOMER
    MetaCyc:MONOMER-17985
    MSME246196:G1H7P-6218-MONOMER

    Family and domain databases

    Gene3Di1.20.120.450, 1 hit
    3.90.1580.10, 1 hit
    HAMAPiMF_02035 EgtB, 1 hit
    InterProiView protein in InterPro
    IPR016187 CTDL_fold
    IPR024775 DinB-like
    IPR034660 DinB/YfiT-like
    IPR017806 EgtB
    IPR032890 EgtB_Actinobacteria
    IPR005532 SUMF_dom
    IPR042095 SUMF_sf
    PfamiView protein in Pfam
    PF12867 DinB_2, 1 hit
    PF03781 FGE-sulfatase, 1 hit
    SUPFAMiSSF109854 SSF109854, 1 hit
    SSF56436 SSF56436, 1 hit
    TIGRFAMsiTIGR03440 egtB_TIGR03440, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEGTB_MYCS2
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0R5N0
    Secondary accession number(s): I7GG92
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: January 9, 2007
    Last modified: December 11, 2019
    This is version 88 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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