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Entry version 88 (16 Oct 2019)
Sequence version 1 (09 Jan 2007)
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Protein

Histidine N-alpha-methyltransferase

Gene

egtD

Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the SAM-dependent triple methylation of the alpha-amino group of histidine to form hercynine, a step in the biosynthesis pathway of ergothioneine. Among all the proteinogenic amino acids, only L-histidine is a substrate.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.58 sec(-1) for the methylation of L-histidine. kcat is 0.23 sec(-1) for the methylation of N-alpha-methyl-L-histidine. kcat is 0.23 sec(-1) for the methylation of N-alpha,N-alpha-dimethyl-L-histidine.1 Publication
  1. KM=109 µM for L-histidine1 Publication
  2. KM=17.7 µM for N-alpha-methyl-L-histidine1 Publication
  3. KM=25.3 µM for N-alpha,N-alpha-dimethyl-L-histidine1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: ergothioneine biosynthesis

    This protein is involved in the pathway ergothioneine biosynthesis, which is part of Amino-acid biosynthesis.1 Publication1 Publication
    View all proteins of this organism that are known to be involved in the pathway ergothioneine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei56L-histidineCombined sources1 Publication1 Publication1
    Binding sitei86S-adenosyl-L-methionine; via carbonyl oxygen2 Publications1
    Binding sitei92S-adenosyl-L-methionine2 Publications1
    Binding sitei113S-adenosyl-L-methionine2 Publications1
    Binding sitei166L-histidineCombined sources1 Publication1 Publication1
    Binding sitei206L-histidineCombined sources1 Publication1 Publication1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionMethyltransferase, Transferase
    LigandS-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    GCF_000015005:MSMEG_6247-MONOMER
    MetaCyc:MONOMER-17984
    MSME246196:G1H7P-6216-MONOMER

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA01014

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Histidine N-alpha-methyltransferaseCurated (EC:2.1.1.442 Publications)
    Alternative name(s):
    Histidine trimethyltransferase1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:egtD1 Publication
    Ordered Locus Names:MSMEG_6247, MSMEI_6086
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri246196 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000006158 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Cells lacking this gene are unable to synthesize ergothioneine (ERG), but do not show growth defect. Deletion of egtD from wild-type M.smegmatis and a mycothiol (MSH)-deficient mutant does not affect their susceptibility to tested antibiotics. The ERG- and MSH-deficient double mutant is significantly more sensitive to peroxide than either of the single mutants lacking either ERG or MSH, suggesting that both thiols play a role in protecting M.smegmatis against oxidative stress and that ERG is able to partly compensate for the loss of MSH.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi252M → V: Dramatic change in substrate specificity since the tryptophan-specific activity is increased more than 2000-fold and the histidine-specific activity is reduced 3000-fold; when associated with A-282. 1 Publication1
    Mutagenesisi282E → A: 130-fold reduction in catalytic efficiency. Dramatic change in substrate specificity since the tryptophan-specific activity is increased more than 2000-fold and the histidine-specific activity is reduced 3000-fold; when associated with V-252. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004136511 – 321Histidine N-alpha-methyltransferaseAdd BLAST321

    Proteomic databases

    PRoteomics IDEntifications database

    More...
    PRIDEi
    A0R5M8

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Monomer.

    2 Publications

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    246196.MSMEI_6086

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1321
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    A0R5M8

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    Protein Data Bank in Europe - Knowledge Base

    More...
    PDBe-KBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni141 – 142S-adenosyl-L-methionine bindingCombined sources2 Publications2
    Regioni282 – 284L-histidine bindingCombined sources1 Publication1 Publication3

    <p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    Consists of two distinct domains: a typical methyltransferase domain and a unique substrate binding domain.1 Publication

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG41067HS Bacteria
    COG4301 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000253490

    KEGG Orthology (KO)

    More...
    KOi
    K18911

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    RVEMHLV

    Database of Orthologous Groups

    More...
    OrthoDBi
    775492at2

    Family and domain databases

    HAMAP database of protein families

    More...
    HAMAPi
    MF_02037 EgtD, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR035094 EgtD
    IPR032888 EgtD_Actinobacteria
    IPR019257 MeTrfase_dom
    IPR017804 MeTrfase_EgtD-like
    IPR029063 SAM-dependent_MTases

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF10017 Methyltransf_33, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF018005 UCP018005, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF53335 SSF53335, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR03438 egtD_ergothio, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    A0R5M8-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTLSLANYLA ADSAAEALRR DVRAGLTAAP KSLPPKWFYD AVGSDLFDQI
    60 70 80 90 100
    TRLPEYYPTR TEAQILRTRS AEIIAAAGAD TLVELGSGTS EKTRMLLDAM
    110 120 130 140 150
    RDAELLRRFI PFDVDAGVLR SAGAAIGAEY PGIEIDAVCG DFEEHLGKIP
    160 170 180 190 200
    HVGRRLVVFL GSTIGNLTPA PRAEFLSTLA DTLQPGDSLL LGTDLVKDTG
    210 220 230 240 250
    RLVRAYDDAA GVTAAFNRNV LAVVNRELSA DFDLDAFEHV AKWNSDEERI
    260 270 280 290 300
    EMWLRARTAQ HVRVAALDLE VDFAAGEEML TEVSCKFRPE NVVAELAEAG
    310 320
    LRQTHWWTDP AGDFGLSLAV R
    Length:321
    Mass (Da):35,003
    Last modified:January 9, 2007 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i43FE091F6B4A48D6
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK75457.1
    CP001663 Genomic DNA Translation: AFP42517.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_011731156.1, NZ_SIJM01000027.1
    YP_890466.1, NC_008596.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    ABK75457; ABK75457; MSMEG_6247
    AFP42517; AFP42517; MSMEI_6086

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    4537704

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    msb:LJ00_30890
    msg:MSMEI_6086
    msm:MSMEG_6247

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|246196.19.peg.6086

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK75457.1
    CP001663 Genomic DNA Translation: AFP42517.1
    RefSeqiWP_011731156.1, NZ_SIJM01000027.1
    YP_890466.1, NC_008596.1

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4PIMX-ray1.75A/B1-321[»]
    4PINX-ray1.90A/B1-321[»]
    4PIOX-ray1.51A/B1-321[»]
    4PIPX-ray1.80A/B/C/D1-321[»]
    4UY5X-ray2.00A1-321[»]
    4UY6X-ray2.04A2-321[»]
    4UY7X-ray2.31A/B2-321[»]
    6FNQX-ray1.75A/B3-321[»]
    6FNRX-ray1.83A/B3-321[»]
    6FNSX-ray1.85A/B3-321[»]
    6FNTX-ray1.90A/B3-321[»]
    SMRiA0R5M8
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    STRINGi246196.MSMEI_6086

    Proteomic databases

    PRIDEiA0R5M8

    Genome annotation databases

    EnsemblBacteriaiABK75457; ABK75457; MSMEG_6247
    AFP42517; AFP42517; MSMEI_6086
    GeneIDi4537704
    KEGGimsb:LJ00_30890
    msg:MSMEI_6086
    msm:MSMEG_6247
    PATRICifig|246196.19.peg.6086

    Phylogenomic databases

    eggNOGiENOG41067HS Bacteria
    COG4301 LUCA
    HOGENOMiHOG000253490
    KOiK18911
    OMAiRVEMHLV
    OrthoDBi775492at2

    Enzyme and pathway databases

    UniPathwayiUPA01014
    BioCyciGCF_000015005:MSMEG_6247-MONOMER
    MetaCyc:MONOMER-17984
    MSME246196:G1H7P-6216-MONOMER

    Family and domain databases

    HAMAPiMF_02037 EgtD, 1 hit
    InterProiView protein in InterPro
    IPR035094 EgtD
    IPR032888 EgtD_Actinobacteria
    IPR019257 MeTrfase_dom
    IPR017804 MeTrfase_EgtD-like
    IPR029063 SAM-dependent_MTases
    PfamiView protein in Pfam
    PF10017 Methyltransf_33, 1 hit
    PIRSFiPIRSF018005 UCP018005, 1 hit
    SUPFAMiSSF53335 SSF53335, 1 hit
    TIGRFAMsiTIGR03438 egtD_ergothio, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEGTD_MYCS2
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0R5M8
    Secondary accession number(s): I7GAE1
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: January 9, 2007
    Last modified: October 16, 2019
    This is version 88 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
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