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UniProtKB - A0R5K5 (LFRA_MYCS2)

Entry version 100 (23 Feb 2022)
Sequence version 1 (09 Jan 2007)
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Protein

Multidrug efflux pump LfrA

Gene

lfrA

Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Energy-dependent efflux pump that contributes to drug resistance (PubMed:8682782, PubMed:11094273, PubMed:15215089, PubMed:21332993).

Catalyzes the efflux of norfloxacin and several related fluoroquinolones (FQ) (PubMed:8682782).

Contributes significantly to the intrinsic MICs for ethidium bromide and acriflavine (PubMed:11094273, PubMed:21332993).

Overexpression confers low-level resistance to hydrophilic FQ such as ciprofloxacin, ofloxacin and levofloxacin, and to ethidium bromide, acridine, acriflavine, rhodamine 123 and some quaternary ammonium compounds (PubMed:8552639, PubMed:11094273).

May contribute to resistance to certain beta-lactams (PubMed:15215089).

Probably uses the proton motive force (PubMed:8682782).

5 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by the protonophore carbonyl cyanide m-chorophenylhydrazone (CCCP) (PubMed:8682782). Ethidium bromide efflux is inhibited by chlorpromazine, thioridazine and verapamil (PubMed:21332993).2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAntibiotic resistance, Transport

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.1.3.3, the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Multidrug efflux pump LfrACurated
Alternative name(s):
Low-level fluoroquinolone resistance protein1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:lfrA1 Publication
Ordered Locus Names:MSMEG_6225Imported, MSMEI_6063Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri246196 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006158 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Transmembranei58 – 78HelicalSequence analysisAdd BLAST21
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Transmembranei145 – 165HelicalSequence analysisAdd BLAST21
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Transmembranei309 – 329HelicalSequence analysisAdd BLAST21
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Transmembranei361 – 381HelicalSequence analysisAdd BLAST21
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21
Transmembranei480 – 500HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Disruption or deletion of the gene decreases resistance to ethidium bromide and acriflavine and results in a small decrease in minimal inhibitory concentrations (MICs) for ciprofloxacin, doxorubicin, rhodamine, norfloxacin, isoniazid, tetracycline, ethambutol and rifampicin.3 Publications

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL4295530

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004473201 – 504Multidrug efflux pump LfrAAdd BLAST504

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expression is repressed by LfrR (PubMed:15215089, PubMed:17043130). Induced by ethidium bromide, acriflavine or rhodamine 123, via inhibition of LfrR binding (PubMed:17043130).2 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...STRINGi		246196.MSMEI_6063

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		A0R5K5

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0477, Bacteria

Identification of Orthologs from Complete Genome Data

More...OMAi		NLPVMAV

Database of Orthologous Groups

More...OrthoDBi		626204at2

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1250.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR011701, MFS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF07690, MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF103473, SSF103473, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50850, MFS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A0R5K5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTCIEGTPS TTRTPTRAWV ALAVLALPVL LIAIDNTVLA FALPLIAEDF 
        60         70         80         90        100
RPSATTQLWI VDVYSLVLAA LLVAMGSLGD RLGRRRLLLI GGAGFAVVSA 
       110        120        130        140        150
LAAFAPSAEL LVGARALLGV FGAMLMPSTL SLIRNIFTDA SARRLAIAIW 
       160        170        180        190        200
ASCFTAGSAL GPIVGGALLE HFHWGAVFLV AVPILLPLLV LGPRLVPESR 
       210        220        230        240        250
DPNPGPFDPV SIVLSFTTML PIVWAVKTAA HDGLSAAAAA AFAVGIVSGA 
       260        270        280        290        300
LFVRRQNRSA TPMLDIGLFK VMPFTSSILA NFLSIIGLIG FIFFISQHLQ 
       310        320        330        340        350
LVLGLSPLTA GLVTLPGAVV SMIAGLAVVK AAKRFAPDTL MVTGLVFVAV 
       360        370        380        390        400
GFLMILLFRH NLTVAAIIAS FVVLELGVGV SQTVSNDTIV ASVPAAKSGA 
       410        420        430        440        450
ASAVSETAYE LGAVVGTATL GTIFTAFYRS NVDVPAGLTP EQTGAAAESI 
       460        470        480        490        500
GGAAAVAADL PAATATQLLD SARAAFDSGI APTAVIAAML VLAAAAVVGV 

AFRR
Length:504
Mass (Da):51,524
Last modified:January 9, 2007 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7CBFF4468811FE75
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti87L → V in AAC43550 (PubMed:8552639).Curated1
Sequence conflicti108A → T in AAC43550 (PubMed:8552639).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U40487 Genomic DNA Translation: AAC43550.1
CP000480 Genomic DNA Translation: ABK70581.1
CP001663 Genomic DNA Translation: AFP42494.1

NCBI Reference Sequences

More...RefSeqi		WP_011731134.1, NZ_SIJM01000027.1
YP_890443.1, NC_008596.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		ABK70581; ABK70581; MSMEG_6225
AFP42494; AFP42494; MSMEI_6063

Database of genes from NCBI RefSeq genomes

More...GeneIDi		66737507

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		msg:MSMEI_6063
msm:MSMEG_6225

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|246196.19.peg.6062

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40487 Genomic DNA Translation: AAC43550.1
CP000480 Genomic DNA Translation: ABK70581.1
CP001663 Genomic DNA Translation: AFP42494.1
RefSeqiWP_011731134.1, NZ_SIJM01000027.1
YP_890443.1, NC_008596.1

3D structure databases

SMRiA0R5K5
ModBaseiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEI_6063

Chemistry databases

ChEMBLiCHEMBL4295530

Protein family/group databases

TCDBi2.A.1.3.3, the major facilitator superfamily (mfs)

Genome annotation databases

EnsemblBacteriaiABK70581; ABK70581; MSMEG_6225
AFP42494; AFP42494; MSMEI_6063
GeneIDi66737507
KEGGimsg:MSMEI_6063
msm:MSMEG_6225
PATRICifig|246196.19.peg.6062

Phylogenomic databases

eggNOGiCOG0477, Bacteria
OMAiNLPVMAV
OrthoDBi626204at2

Family and domain databases

Gene3Di1.20.1250.20, 1 hit
InterProiView protein in InterPro
IPR011701, MFS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf
PfamiView protein in Pfam
PF07690, MFS_1, 1 hit
SUPFAMiSSF103473, SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS50850, MFS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiLFRA_MYCS2
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0R5K5
Secondary accession number(s): I7GG83, Q50392
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 5, 2019
Last sequence update: January 9, 2007
Last modified: February 23, 2022
This is version 100 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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