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UniProtKB - A0R4Y3 (CP125_MYCS2)
Protein
Steroid C26-monooxygenase
Gene
cyp125
Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Functioni
Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26-hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4-en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one.
1 PublicationCatalytic activityi
- cholest-4-en-3-one + 5 H+ + 3 O2 + 6 reduced [2Fe-2S]-[ferredoxin] = (25S)-3-oxocholest-4-en-26-oate + 4 H2O + 6 oxidized [2Fe-2S]-[ferredoxin]By similarity1 PublicationEC:1.14.15.29By similarity1 Publication
Cofactori
heme2 Publications
Kineticsi
- KM=14 µM for cholest-4-en-3-one1 Publication
: cholesterol degradation Pathwayi
This protein is involved in the pathway cholesterol degradation, which is part of Steroid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway cholesterol degradation and in Steroid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 360 | Iron (heme axial ligand)Combined sources1 Publication | 1 |
GO - Molecular functioni
- cholest-4-en-3-one 26-monooxygenase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- iron ion binding Source: InterPro
GO - Biological processi
- cholesterol catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Cholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism |
Ligand | Heme, Iron, Metal-binding, NAD |
Enzyme and pathway databases
BRENDAi | 1.14.15.29, 3512 |
UniPathwayi | UPA01058 |
Names & Taxonomyi
Protein namesi | Recommended name: Steroid C26-monooxygenase1 Publication (EC:1.14.15.29By similarity1 Publication)Alternative name(s): Cholest-4-en-3-one C26-monooxygenase1 Publication Cholest-4-en-3-one C26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming]By similarity1 Publication Cholesterol C26-monooxygenase1 Publication Cholesterol C26-monooxygenase [(25S)-3beta-hydroxycholest-5-en-26-oate forming]By similarity1 Publication Cytochrome P450 1251 Publication Steroid C27-monooxygenaseBy similarity |
Gene namesi | Name:cyp1251 Publication Synonyms:cyp125A31 Publication Ordered Locus Names:MSMEG_5995Imported |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene show lower accumulation of 26-hydroxycholest-4-en-3-one and cholest-4-en-3-one-26-oate when compared with the wild-type and display a strong induction of cyp142A2. The levels of 26-hydroxycholest-4-en-3-one and cholest-4-on-3-one-26-oate are drastically reduced in cells lacking both cyp125A3 and cyp142A2.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000438723 | 1 – 427 | Steroid C26-monooxygenaseAdd BLAST | 427 |
Expressioni
Inductioni
By cholesterol.1 Publication
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A0R4Y3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | COG2124, Bacteria |
OMAi | QRFILIN |
OrthoDBi | 816674at2 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR002397, Cyt_P450_B IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00359, BP450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
i Sequence
Sequence statusi: Complete.
A0R4Y3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPTPNIPSDF DFLDATLNLE RLPVEELAEL RKSEPIHWVD VPGGTGGFGD
60 70 80 90 100
KGYWLVTKHA DVKEVSRRSD VFGSSPDGAI PVWPQDMTRE AVDLQRAVLL
110 120 130 140 150
NMDAPQHTRL RKIISRGFTP RAIGRLEDEL RSRAQKIAQT AAAQGAGDFV
160 170 180 190 200
EQVSCELPLQ AIAELLGVPQ DDRDKLFRWS NEMTAGEDPE YADVDPAMSS
210 220 230 240 250
FELISYAMKM AEERAVNPTE DIVTKLIEAD IDGEKLSDDE FGFFVVMLAV
260 270 280 290 300
AGNETTRNSI THGMIAFAQN PDQWELYKKE RPETAADEIV RWATPVSAFQ
310 320 330 340 350
RTALEDVELG GVQIKKGQRV VMSYRSANFD EEVFEDPHTF NILRSPNPHV
360 370 380 390 400
GFGGTGAHYC IGANLARMTI NLIFNAIADN MPDLKPIGAP ERLKSGWLNG
410 420
IKHWQVDYTG AGKASVSGAP GTCPVAH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 | M → MHFEERTPM in AFP42267 (PubMed:17295914).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK74881.1 CP001663 Genomic DNA Translation: AFP42267.1 |
RefSeqi | WP_011730960.1, NZ_SIJM01000017.1 YP_890221.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK74881; ABK74881; MSMEG_5995 AFP42267; AFP42267; MSMEI_5834 |
GeneIDi | 66737280 |
KEGGi | msg:MSMEI_5834 msm:MSMEG_5995 |
PATRICi | fig|246196.19.peg.5831 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK74881.1 CP001663 Genomic DNA Translation: AFP42267.1 |
RefSeqi | WP_011730960.1, NZ_SIJM01000017.1 YP_890221.1, NC_008596.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4APY | X-ray | 2.00 | A | 1-427 | [»] | |
5DQN | X-ray | 2.26 | A | 1-426 | [»] | |
SMRi | A0R4Y3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_5834 |
Genome annotation databases
EnsemblBacteriai | ABK74881; ABK74881; MSMEG_5995 AFP42267; AFP42267; MSMEI_5834 |
GeneIDi | 66737280 |
KEGGi | msg:MSMEI_5834 msm:MSMEG_5995 |
PATRICi | fig|246196.19.peg.5831 |
Phylogenomic databases
eggNOGi | COG2124, Bacteria |
OMAi | QRFILIN |
OrthoDBi | 816674at2 |
Enzyme and pathway databases
UniPathwayi | UPA01058 |
BRENDAi | 1.14.15.29, 3512 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR002397, Cyt_P450_B IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00359, BP450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP125_MYCS2 | |
Accessioni | A0R4Y3Primary (citable) accession number: A0R4Y3 Secondary accession number(s): I7G9K1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 18, 2017 |
Last sequence update: | January 9, 2007 | |
Last modified: | February 23, 2022 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families