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Entry version 77 (08 May 2019)
Sequence version 1 (09 Jan 2007)
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Protein

Trehalase

Gene

MSMEG_4535

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the hydrolysis of alpha,alpha-trehalose into two molecules of D-glucose. Does not hydrolyze maltose, isomaltose, sucrose, cellobiose, p-nitrophenyl-alpha-D-glucopyranoside, and methyl-alpha-D-glucopyranoside. Is also inactive on alpha,beta-trehalose, beta,beta-trehalose, alpha,alpha-trehalose-6,6'-dibehenate, trehalulose, nigerose, and trehalose dimycolate.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by pyrophosphate and polyphosphates. Also competitively inhibited by validoxylamine and castanospermine, but not by trehazolin.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=20 mM for alpha,alpha-trehalose1 Publication

    pH dependencei

    Optimum pH is 7.1. The trehalase activity drops sharply at pH values of 6.5 and below, as well as at pH values of 8.0 and above.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: trehalose degradation

    This protein is involved in step 1 of the subpathway that synthesizes D-glucose from alpha,alpha-trehalose.
    Proteins known to be involved in this subpathway in this organism are:
    1. Trehalase (MSMEG_4535)
    This subpathway is part of the pathway trehalose degradation, which is itself part of Glycan degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose from alpha,alpha-trehalose, the pathway trehalose degradation and in Glycan degradation.

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism
    LigandMagnesium

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    GCF_000015005:MSMEG_4535-MONOMER
    MSME246196:G1H7P-4511-MONOMER

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00300;UER00535

    Protein family/group databases

    Carbohydrate-Active enZymes

    More...
    CAZyi
    GH15 Glycoside Hydrolase Family 15

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Trehalase (EC:3.2.1.28)
    Alternative name(s):
    Alpha,alpha-trehalase
    Alpha,alpha-trehalose glucohydrolase
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Ordered Locus Names:MSMEG_4535, MSMEI_4422
    ORF Names:MSMEG4528
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri246196 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000006158 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004139791 – 668TrehalaseAdd BLAST668

    Proteomic databases

    PRoteomics IDEntifications database

    More...
    PRIDEi
    A0R0W9

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homomultimer of 20 or more subunits.1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    246196.MSMEI_4422

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105BZP Bacteria
    COG3387 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000243980

    KEGG Orthology (KO)

    More...
    KOi
    K22934

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    NWDYRAS

    Database of Orthologous Groups

    More...
    OrthoDBi
    318658at2

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.50.10.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR008928 6-hairpin_glycosidase_sf
    IPR012341 6hp_glycosidase-like_sf
    IPR011613 GH15-like

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00723 Glyco_hydro_15, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF48208 SSF48208, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    A0R0W9-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVLQQTEPTD GADRKASDGP LTVTAPVPYA AGPTLRNPFP PIADYGFLSD
    60 70 80 90 100
    CETTCLISSA GSVEWLCVPR PDSPSVFGAI LDRGAGHFRL GPYGVSVPAA
    110 120 130 140 150
    RRYLPGSLIL ETTWQTHTGW LIVRDALVMG PWHDIDTRSR THRRTPMDWD
    160 170 180 190 200
    AEHILLRTVR CVSGTVELVM SCEPAFDYHR VSATWEYSGP AYGEAIARAS
    210 220 230 240 250
    RNPDSHPTLR LTTNLRIGIE GREARARTRL TEGDNVFVAL SWSKHPAPQT
    260 270 280 290 300
    YEEAADKMWK TSEAWRQWIN VGDFPDHPWR AYLQRSALTL KGLTYSPTGA
    310 320 330 340 350
    LLAAPTTSLP ETPQGERNWD YRYSWIRDST FALWGLYTLG LDREADDFFS
    360 370 380 390 400
    FIADVSGANN GERHPLQVMY GVGGERSLVE EELHHLSGYD NSRPVRIGNG
    410 420 430 440 450
    AYNQRQHDIW GTMLDSVYLH AKSREQIPDA LWPVLKNQVE EAIKHWKEPD
    460 470 480 490 500
    RGIWEVRGEP QHFTSSKIMC WVALDRGSKL AELQGEKSYA QQWRAIAEEI
    510 520 530 540 550
    KADVLARGVD KRGVLTQRYG DDALDASLLL AVLTRFLPAD DPRIRATVLA
    560 570 580 590 600
    IADELTEDGL VLRYRVEETD DGLAGEEGTF TICSFWLVSA LVEIGEISRA
    610 620 630 640 650
    KHLCERLLSF ASPLHLYAEE IEPRTGRHLG NFPQAFTHLA LINAVVHVIR
    660
    AEEEADSSGV FVPANAPM
    Length:668
    Mass (Da):74,690
    Last modified:January 9, 2007 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i22AE1581E85F503A
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK72415.1
    CP001663 Genomic DNA Translation: AFP40876.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_011729900.1, NZ_SIJM01000052.1
    YP_888807.1, NC_008596.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    ABK72415; ABK72415; MSMEG_4535
    AFP40876; AFP40876; MSMEI_4422

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    4533923

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    msb:LJ00_22440
    msg:MSMEI_4422
    msm:MSMEG_4535

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|246196.19.peg.4437

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK72415.1
    CP001663 Genomic DNA Translation: AFP40876.1
    RefSeqiWP_011729900.1, NZ_SIJM01000052.1
    YP_888807.1, NC_008596.1

    3D structure databases

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    SWISS-MODEL Interactive Workspace

    More...
    SWISS-MODEL-Workspacei
    Submit a new modelling project...

    Protein-protein interaction databases

    STRINGi246196.MSMEI_4422

    Protein family/group databases

    CAZyiGH15 Glycoside Hydrolase Family 15

    Proteomic databases

    PRIDEiA0R0W9

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABK72415; ABK72415; MSMEG_4535
    AFP40876; AFP40876; MSMEI_4422
    GeneIDi4533923
    KEGGimsb:LJ00_22440
    msg:MSMEI_4422
    msm:MSMEG_4535
    PATRICifig|246196.19.peg.4437

    Phylogenomic databases

    eggNOGiENOG4105BZP Bacteria
    COG3387 LUCA
    HOGENOMiHOG000243980
    KOiK22934
    OMAiNWDYRAS
    OrthoDBi318658at2

    Enzyme and pathway databases

    UniPathwayiUPA00300;UER00535
    BioCyciGCF_000015005:MSMEG_4535-MONOMER
    MSME246196:G1H7P-4511-MONOMER

    Family and domain databases

    Gene3Di1.50.10.10, 1 hit
    InterProiView protein in InterPro
    IPR008928 6-hairpin_glycosidase_sf
    IPR012341 6hp_glycosidase-like_sf
    IPR011613 GH15-like
    PfamiView protein in Pfam
    PF00723 Glyco_hydro_15, 1 hit
    SUPFAMiSSF48208 SSF48208, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTREH_MYCS2
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0R0W9
    Secondary accession number(s): I7GCG8
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
    Last sequence update: January 9, 2007
    Last modified: May 8, 2019
    This is version 77 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
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