UniProtKB - A0R0S4 (DPDS_MYCS2)
Protein
Decaprenyl diphosphate synthase
Gene
uppS
Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Functioni
Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) in the cis configuration with (2Z,6E)-farnesyl diphosphate (Z-FPP or EZ-FPP) generating the 50 carbon product trans,polycis-decaprenyl diphosphate. When (2E,6E)-farnesyl diphosphate (E-FPP or EE-FPP) is used in vitro, both primary products decaprenyl diphosphate and heptaprenyl diphosphate are synthesized. It is probably due to the fact that M.smegmatis synthesizes both (2E,6E,10E)-geranylgeranyl diphosphate (EEE-GGPP) and (2E,6E,10Z)-geranylgeranyl diphosphate (EEZ-GGPP). Can also accept many different allylic substrates, including E-geranyl diphosphate (E-GPP), neryl diphosphate (NPP), and all-trans-geranyl-geranyl diphosphate.1 Publication
Catalytic activityi
- (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate = (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate + 7 diphosphateEC:2.5.1.86
- (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = di-trans,poly-cis-polyprenyl diphosphate + n diphosphateEC:2.5.1.87
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 73 | By similarity | 1 | |
Metal bindingi | 73 | MagnesiumBy similarity | 1 | |
Binding sitei | 78 | SubstrateBy similarity | 1 | |
Binding sitei | 86 | SubstrateBy similarity | 1 | |
Binding sitei | 90 | SubstrateBy similarity | 1 | |
Active sitei | 121 | Proton acceptorBy similarity | 1 | |
Binding sitei | 122 | SubstrateBy similarity | 1 | |
Binding sitei | 124 | SubstrateBy similarity | 1 | |
Binding sitei | 241 | SubstrateBy similarity | 1 | |
Metal bindingi | 260 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- di-trans,poly-cis-decaprenylcistransferase activity Source: UniProtKB
- magnesium ion binding Source: UniProtKB-UniRule
- Z-farnesyl diphosphate synthase activity Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | MSME246196:G1H7P-4466-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:uppS Ordered Locus Names:MSMEG_4490, MSMEI_4379 |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
Subcellular locationi
- Cell membrane 1 Publication
GO - Cellular componenti
- plasma membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000417570 | 1 – 293 | Decaprenyl diphosphate synthaseAdd BLAST | 293 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 246196.MSMEI_4379 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 74 – 77 | Substrate bindingBy similarity | 4 | |
Regioni | 118 – 120 | Substrate bindingBy similarity | 3 | |
Regioni | 247 – 249 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the UPP synthase family.Curated
Phylogenomic databases
eggNOGi | COG0020, Bacteria |
OMAi | PRTEGHK |
OrthoDBi | 1630604at2 |
Family and domain databases
CDDi | cd00475, Cis_IPPS, 1 hit |
Gene3Di | 3.40.1180.10, 1 hit |
HAMAPi | MF_01139, ISPT, 1 hit |
InterProi | View protein in InterPro IPR001441, UPP_synth-like IPR018520, UPP_synth-like_CS IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR10291, PTHR10291, 1 hit |
Pfami | View protein in Pfam PF01255, Prenyltransf, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
TIGRFAMsi | TIGR00055, uppS, 1 hit |
PROSITEi | View protein in PROSITE PS01066, UPP_SYNTHASE, 1 hit |
i Sequence
Sequence statusi: Complete.
A0R0S4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATTRGKKTY PQLPPAPDDY PTFPDKSTWP VVFPEIPAGT NGRFARPPQH
60 70 80 90 100
TSKAAAPKIP ADQVPNHVAV VMDGNGRWAT QRGLGRTEGH KMGEAVLIDI
110 120 130 140 150
TCGAIEIGIK HLTVYAFSTE NWKRSTEEVR FLMGFNREVV RRRRENLNDM
160 170 180 190 200
GVRMRWVGSR PRMWRSVIKE FDIAEQMTVD NDVITINYCV NYGGRTEIVE
210 220 230 240 250
AARALAQEAV DGKINPARIS EAMFAKHLHR ADIPDVDLFI RTSGEQRASN
260 270 280 290
FLLWQAAYAE YVFQDKLWPD YDRRDLWAAC EEYVNRNRRF GRA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK71014.1 CP001663 Genomic DNA Translation: AFP40833.1 |
RefSeqi | WP_011729873.1, NZ_SIJM01000026.1 YP_888762.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK71014; ABK71014; MSMEG_4490 AFP40833; AFP40833; MSMEI_4379 |
GeneIDi | 45745856 |
KEGGi | msg:MSMEI_4379 msm:MSMEG_4490 |
PATRICi | fig|246196.19.peg.4396 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK71014.1 CP001663 Genomic DNA Translation: AFP40833.1 |
RefSeqi | WP_011729873.1, NZ_SIJM01000026.1 YP_888762.1, NC_008596.1 |
3D structure databases
SMRi | A0R0S4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_4379 |
Genome annotation databases
EnsemblBacteriai | ABK71014; ABK71014; MSMEG_4490 AFP40833; AFP40833; MSMEI_4379 |
GeneIDi | 45745856 |
KEGGi | msg:MSMEI_4379 msm:MSMEG_4490 |
PATRICi | fig|246196.19.peg.4396 |
Phylogenomic databases
eggNOGi | COG0020, Bacteria |
OMAi | PRTEGHK |
OrthoDBi | 1630604at2 |
Enzyme and pathway databases
BioCyci | MSME246196:G1H7P-4466-MONOMER |
Family and domain databases
CDDi | cd00475, Cis_IPPS, 1 hit |
Gene3Di | 3.40.1180.10, 1 hit |
HAMAPi | MF_01139, ISPT, 1 hit |
InterProi | View protein in InterPro IPR001441, UPP_synth-like IPR018520, UPP_synth-like_CS IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR10291, PTHR10291, 1 hit |
Pfami | View protein in Pfam PF01255, Prenyltransf, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
TIGRFAMsi | TIGR00055, uppS, 1 hit |
PROSITEi | View protein in PROSITE PS01066, UPP_SYNTHASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPDS_MYCS2 | |
Accessioni | A0R0S4Primary (citable) accession number: A0R0S4 Secondary accession number(s): I7FQ48 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2012 |
Last sequence update: | January 9, 2007 | |
Last modified: | February 10, 2021 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families