UniProtKB - A0QVY5 (LEXA_MYCS2)
Protein
LexA repressor
Gene
lexA
Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Functioni
Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation
Catalytic activityi
- Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotation EC:3.4.21.88
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 167 | For autocatalytic cleavage activityUniRule annotation | 1 | |
Active sitei | 204 | For autocatalytic cleavage activityUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 56 – 76 | H-T-H motifUniRule annotationAdd BLAST | 21 |
GO - Molecular functioni
- DNA-binding transcription repressor activity Source: CollecTF
- serine-type endopeptidase activity Source: UniProtKB-UniRule
- transcription regulatory region sequence-specific DNA binding Source: CollecTF
GO - Biological processi
- DNA repair Source: UniProtKB-UniRule
- DNA replication Source: UniProtKB-UniRule
- SOS response Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Hydrolase, Repressor |
Biological process | DNA damage, DNA repair, DNA replication, SOS response, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | MSME246196:G1H7P-2739-MONOMER |
Protein family/group databases
MEROPSi | S24.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:lexAUniRule annotation Ordered Locus Names:MSMEG_2740, MSMEI_2673 |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000322745 | 1 – 243 | LexA repressorAdd BLAST | 243 |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 132 – 133 | Cleavage; by autolysisUniRule annotation | 2 |
Keywords - PTMi
Autocatalytic cleavageInteractioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 246196.MSMEI_2673 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S24 family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1974, Bacteria |
OMAi | HVWLLPH |
OrthoDBi | 1933795at2 |
Family and domain databases
CDDi | cd06529, S24_LexA-like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00015, LexA, 1 hit |
InterProi | View protein in InterPro IPR006200, LexA IPR039418, LexA-like IPR036286, LexA/Signal_pep-like_sf IPR006199, LexA_DNA-bd_dom IPR006197, Peptidase_S24_LexA IPR015927, Peptidase_S24_S26A/B/C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01726, LexA_DNA_bind, 1 hit PF00717, Peptidase_S24, 1 hit |
PRINTSi | PR00726, LEXASERPTASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF51306, SSF51306, 1 hit |
TIGRFAMsi | TIGR00498, lexA, 1 hit |
i Sequence
Sequence statusi: Complete.
A0QVY5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDDTGEFTD GSTESPADAD GAGRRRAVDN GLTERQRTIL EVIRASVTSR
60 70 80 90 100
GYPPSIREIG DAVGLTSTSS VAHQLRTLER KGYLRRDPNR PRAVDVRAAD
110 120 130 140 150
DPAAAAVVTT DVAGSDALPE PTFVPVLGRI AAGGPILAEE AVEDVFPLPR
160 170 180 190 200
ELVGEGSLFL LKVVGDSMVD AAICDGDWVV VRQQNVADNG DIVAAMIDGE
210 220 230 240
ATVKTFKRAR GQVWLMPHNP AYDPIPGNEA AVLGKVVTVI RKI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK70187.1 CP001663 Genomic DNA Translation: AFP39141.1 |
RefSeqi | WP_003894124.1, NZ_SIJM01000032.1 YP_887073.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK70187; ABK70187; MSMEG_2740 AFP39141; AFP39141; MSMEI_2673 |
KEGGi | msg:MSMEI_2673 msm:MSMEG_2740 |
PATRICi | fig|246196.19.peg.2708 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK70187.1 CP001663 Genomic DNA Translation: AFP39141.1 |
RefSeqi | WP_003894124.1, NZ_SIJM01000032.1 YP_887073.1, NC_008596.1 |
3D structure databases
SMRi | A0QVY5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_2673 |
Protein family/group databases
MEROPSi | S24.001 |
Genome annotation databases
EnsemblBacteriai | ABK70187; ABK70187; MSMEG_2740 AFP39141; AFP39141; MSMEI_2673 |
KEGGi | msg:MSMEI_2673 msm:MSMEG_2740 |
PATRICi | fig|246196.19.peg.2708 |
Phylogenomic databases
eggNOGi | COG1974, Bacteria |
OMAi | HVWLLPH |
OrthoDBi | 1933795at2 |
Enzyme and pathway databases
BioCyci | MSME246196:G1H7P-2739-MONOMER |
Gene expression databases
CollecTFi | EXPREG_00000170 |
Family and domain databases
CDDi | cd06529, S24_LexA-like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00015, LexA, 1 hit |
InterProi | View protein in InterPro IPR006200, LexA IPR039418, LexA-like IPR036286, LexA/Signal_pep-like_sf IPR006199, LexA_DNA-bd_dom IPR006197, Peptidase_S24_LexA IPR015927, Peptidase_S24_S26A/B/C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01726, LexA_DNA_bind, 1 hit PF00717, Peptidase_S24, 1 hit |
PRINTSi | PR00726, LEXASERPTASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF51306, SSF51306, 1 hit |
TIGRFAMsi | TIGR00498, lexA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEXA_MYCS2 | |
Accessioni | A0QVY5Primary (citable) accession number: A0QVY5 Secondary accession number(s): I7G7A4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | January 9, 2007 | |
Last modified: | February 10, 2021 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families