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Protein

Alanine dehydrogenase

Gene

ald

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible reductive amination of pyruvate to L-alanine. May be required for the adaptation from aerobic growth to anaerobic dormancy. I could be involved in the maintenance of the NAD pool during the shift to an anaerobic dormant state in which oxygen as a terminal electron acceptor becomes limited.1 Publication

Catalytic activityi

L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.

pH dependencei

Optimum pH is between 10 and 11 for the oxidative deamination and between 7 and 7.5 for the reductive amination.1 Publication

Pathwayi: L-alanine degradation via dehydrogenase pathway

This protein is involved in step 1 of the subpathway that synthesizes NH(3) and pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine dehydrogenase (ald)
This subpathway is part of the pathway L-alanine degradation via dehydrogenase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NH(3) and pyruvate from L-alanine, the pathway L-alanine degradation via dehydrogenase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15SubstrateBy similarity1
Binding sitei75SubstrateBy similarity1
Active sitei96Proton donor/acceptorBy similarity1
Binding sitei134NADBy similarity1
Binding sitei198NADBy similarity1
Binding sitei220NADBy similarity1
Active sitei270Proton donor/acceptorBy similarity1
Binding sitei279NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179NADBy similarity2
Nucleotide bindingi239 – 240NADBy similarity2
Nucleotide bindingi267 – 270NADBy similarity4
Nucleotide bindingi298 – 301NADBy similarity4

GO - Molecular functioni

  • alanine dehydrogenase activity Source: UniProtKB
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00527; UER00585

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine dehydrogenase (EC:1.4.1.1)
Gene namesi
Name:ald
Ordered Locus Names:MSMEG_2659, MSMEI_2596
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004196691 – 371Alanine dehydrogenaseAdd BLAST371

Expressioni

Inductioni

StrongLY induced immediately after deflection from aerobic growth.1 Publication

Interactioni

Subunit structurei

Homohexamer. Trimer of dimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_2659

Structurei

3D structure databases

ProteinModelPortaliA0QVQ8
SMRiA0QVQ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Phylogenomic databases

eggNOGiENOG4105DCF Bacteria
COG0686 LUCA
HOGENOMiHOG000022120
KOiK00259
OMAiCFETSKA
OrthoDBiPOG091H02R2

Family and domain databases

CDDicd05305 L-AlaDH, 1 hit
InterProiView protein in InterPro
IPR008141 Ala_DH
IPR008143 Ala_DH/PNT_CS2
IPR008142 AlaDH/PNT_CS1
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR42795 PTHR42795, 1 hit
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PIRSFiPIRSF000183 Alanine_dh, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00518 alaDH, 1 hit
PROSITEiView protein in PROSITE
PS00836 ALADH_PNT_1, 1 hit
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0QVQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVGIPTEIK NNEYRVAITP AGVAELTRRG HEVIIQAGAG EGSAISDRDF
60 70 80 90 100
KAAGAEIVNT ADQVWSEAEL LLKVKEPIEP EYSRMRKGQT LFTYLHLAAS
110 120 130 140 150
KPCTDALLAS GTTSIAYETV QTAEGALPLL APMSEVAGRL SAQVGAYHLM
160 170 180 190 200
RSYGGRGVLM GGVPGVAPAE VVVIGAGTAG YNAARVAAGM GAHVTVFDLN
210 220 230 240 250
INTLRRVDGE FGGRIETRYS SSLELEEAVK KADLVIGAVL VPGAKAPKLV
260 270 280 290 300
TNSTVAHMKP GAVLVDIAID QGGCFEDSRP TTHDEPTFKV HDTIFYCVAN
310 320 330 340 350
MPGAVPRTST FALTNSTMPY VLKLADKGWQ AACASDSALA KGLSTHDGKL
360 370
LSEAVAKDLD LPFTDAAQFL A
Length:371
Mass (Da):38,955
Last modified:January 9, 2007 - v1
Checksum:iA8010101B8EABDCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK70995.1
CP001663 Genomic DNA Translation: AFP39064.1
RefSeqiWP_003894041.1, NZ_CP009494.1
YP_886996.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK70995; ABK70995; MSMEG_2659
AFP39064; AFP39064; MSMEI_2596
GeneIDi4534068
KEGGimsb:LJ00_13235
msg:MSMEI_2596
msm:MSMEG_2659
PATRICifig|246196.56.peg.2659

Similar proteinsi

Entry informationi

Entry nameiDHA_MYCS2
AccessioniPrimary (citable) accession number: A0QVQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: January 9, 2007
Last modified: October 25, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome