UniProtKB - A0QS30 (RECC_MYCS2)
RecBCD enzyme subunit RecC
recC
Functioni
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit recognizes the wild-type Chi sequence, and when added to isolated RecB increases its ATP-dependent helicase processivity (By similarity).
Unlike the case in E.coli, suppresses RecA-dependent homologous recombination, is instead required for single-strand annealing pathway repair of DSB.
By similarity1 PublicationCatalytic activityi
- Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.UniRule annotation EC:3.1.11.5
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- DNA helicase activity Source: UniProtKB-UniRule
- exodeoxyribonuclease V activity Source: UniProtKB-EC
GO - Biological processi
- double-strand break repair via homologous recombination Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: RecBCD enzyme subunit RecCUniRule annotation (EC:3.1.11.5UniRule annotation)Alternative name(s): Exonuclease V subunit RecCUniRule annotation Short name: ExoV subunit RecCUniRule annotation Helicase/nuclease RecBCD subunit RecCUniRule annotation |
Gene namesi | Name:recCUniRule annotation Ordered Locus Names:MSMEG_1328 |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000425942 | 1 – 1085 | RecBCD enzyme subunit RecCAdd BLAST | 1085 |
Interactioni
Subunit structurei
Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding.
UniRule annotationProtein-protein interaction databases
STRINGi | 246196.MSMEI_1290 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1330, Bacteria |
OrthoDBi | 1320159at2 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit 3.40.50.300, 2 hits |
HAMAPi | MF_01486, RecC, 1 hit |
InterProi | View protein in InterPro IPR013986, DExx_box_DNA_helicase_dom_sf IPR027417, P-loop_NTPase IPR006697, RecC IPR041500, RecC_C IPR011335, Restrct_endonuc-II-like |
Pfami | View protein in Pfam PF17946, RecC_C, 1 hit |
PIRSFi | PIRSF000980, RecC, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF52980, SSF52980, 1 hit |
TIGRFAMsi | TIGR01450, recC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MALHLHRAER TDLLADGLAA LLSDPLPDPF ATELVLVPAK GVERWLSQRL
60 70 80 90 100
SNRLGVCAAV EFRNPRSLIA ELTGTADDDP WSPDAMVWPL LGVIDESLDE
110 120 130 140 150
PWCATLATHL GHFEMGEEYE LRQGRRYAVA RRLAGLFASY ARQRPQLLVD
160 170 180 190 200
WDGLPDDLAW QKALWQALCE RIDADPPHIR HAKTLVQLHE SPTDLPQRLS
210 220 230 240 250
LFGHTRLPAT EVELLTALAT HHDLHLWLPH PSDTLWQALS TRKGPLPRRE
260 270 280 290 300
DTSHRDVGHP LLATLGRDLR EMQRLLPEPD TDEFRGRSEY PDTLLGWLQS
310 320 330 340 350
DIAADAVRPQ GRHHRREDRS IQVHSCHGPS RQIDVLREVL LGLLADDPTL
360 370 380 390 400
EPRDILVMCP DIETYAPLIT AGFGLGDVVP RTRGAHINLS AHPAHKLRVR
410 420 430 440 450
LADRALVQTN PLLAVAAQLL ALAGGRATAS EVLNLAESAP VRARFGFTDD
460 470 480 490 500
DLDAITAWTR EANIRWGFDQ EHRRPYGVEF LHNTWRFGID RVLAGVAMSD
510 520 530 540 550
DSHAWLGTTL PLDDVSSNRV ELAGRFADFV EKLRRTVDQL SGTRPLHEWL
560 570 580 590 600
DALTAGIDAL ALADEDWPAA QMQREFAEIT ARAGDTATSL RLADVRALLD
610 620 630 640 650
RHLAGRPTRA NFRTGTLTVC TMVPMRSVPH RVVCLVGLDD GVFPRLGAVD
660 670 680 690 700
GDDALARDPM TGERDIRSED RQLLLDAILA ATQTLVITYT GANEYSGQAR
710 720 730 740 750
PPAVPLAELI DTLKITTEDS ALDVLTRHPL QPFDKRNVIP GALVPDEPFT
760 770 780 790 800
FDQTALVAAR ASSGERAMRP AFFSDPLPPP LPDDVALEDL LNFFKDPVKG
810 820 830 840 850
FFRALDYTLP WDVEGVSDVM PVDIDALEEW TVGDRMLSDI LRGMTPAAAQ
860 870 880 890 900
QAEWRRGTLP PGQLGWRRAI ALRDQCALLA AEALGFRDTD GQAYDVDIDL
910 920 930 940 950
GGGRRLTGTV SPVFGDRLVS VTYSKLGGKH LLQSWIPLLA LAAGYPDRDW
960 970 980 990 1000
LAVCIGRPAR GTTPRVEGLG GPDNPVDLLA DLVAIYDAGR REPLPLPVKT
1010 1020 1030 1040 1050
SYAWAEARHC GDDPEQKAGF RWRSGRYPGE DAEPAHVRAW GRDAWLRDLM
1060 1070 1080
QPLRPGEEFE GETHRLGAYS SRLWLPLLRA ERPVR
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK73955.1 Different initiation. CP001663 Genomic DNA Translation: AFP37766.1 |
RefSeqi | WP_014877040.1, NZ_SIJM01000030.1 YP_885718.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK73955; ABK73955; MSMEG_1328 AFP37766; AFP37766; MSMEI_1290 |
GeneIDi | 66732788 |
KEGGi | msg:MSMEI_1290 msm:MSMEG_1328 |
PATRICi | fig|246196.19.peg.1315 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000480 Genomic DNA Translation: ABK73955.1 Different initiation. CP001663 Genomic DNA Translation: AFP37766.1 |
RefSeqi | WP_014877040.1, NZ_SIJM01000030.1 YP_885718.1, NC_008596.1 |
3D structure databases
SMRi | A0QS30 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_1290 |
Genome annotation databases
EnsemblBacteriai | ABK73955; ABK73955; MSMEG_1328 AFP37766; AFP37766; MSMEI_1290 |
GeneIDi | 66732788 |
KEGGi | msg:MSMEI_1290 msm:MSMEG_1328 |
PATRICi | fig|246196.19.peg.1315 |
Phylogenomic databases
eggNOGi | COG1330, Bacteria |
OrthoDBi | 1320159at2 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit 3.40.50.300, 2 hits |
HAMAPi | MF_01486, RecC, 1 hit |
InterProi | View protein in InterPro IPR013986, DExx_box_DNA_helicase_dom_sf IPR027417, P-loop_NTPase IPR006697, RecC IPR041500, RecC_C IPR011335, Restrct_endonuc-II-like |
Pfami | View protein in Pfam PF17946, RecC_C, 1 hit |
PIRSFi | PIRSF000980, RecC, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF52980, SSF52980, 1 hit |
TIGRFAMsi | TIGR01450, recC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RECC_MYCS2 | |
Accessioni | A0QS30Primary (citable) accession number: A0QS30 Secondary accession number(s): I7G3M1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 19, 2014 |
Last sequence update: | March 19, 2014 | |
Last modified: | February 23, 2022 | |
This is version 105 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families