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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Kineticsi

  1. KM=1.6 µM for S-methyl-5'-thioadenosine1 Publication
  2. KM=310 µM for adenosine1 Publication

    Pathwayi: L-methionine biosynthesis via salvage pathway

    This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. S-methyl-5'-thioadenosine phosphorylase (mtnP)
    This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei9PhosphateUniRule annotation1
    Sitei157Important for substrate specificityUniRule annotation1
    Binding sitei175Substrate; via amide nitrogenUniRule annotation1
    Binding sitei176PhosphateUniRule annotation1
    Sitei212Important for substrate specificityUniRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processPurine salvage

    Enzyme and pathway databases

    UniPathwayi
    UPA00904;UER00873

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylaseUniRule annotation
    Short name:
    MTA phosphorylaseUniRule annotation
    Short name:
    MTAPUniRule annotation
    Gene namesi
    Name:mtnPUniRule annotation
    Synonyms:pnp
    Ordered Locus Names:MSMEG_0990, MSMEI_0963
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
    Proteomesi
    • UP000006158 Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004150951 – 259S-methyl-5'-thioadenosine phosphorylaseAdd BLAST259

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246196.MSMEG_0990

    Structurei

    3D structure databases

    ProteinModelPortaliA0QR54
    SMRiA0QR54
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni50 – 51Phosphate bindingUniRule annotation2
    Regioni199 – 201Substrate bindingUniRule annotation3

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4107USC Bacteria
    COG0005 LUCA
    HOGENOMiHOG000228987
    KOiK00772
    OMAiNLYRSWG
    OrthoDBiPOG091H0228

    Family and domain databases

    HAMAPiMF_01963 MTAP, 1 hit
    InterProiView protein in InterPro
    IPR010044 MTAP
    IPR000845 Nucleoside_phosphorylase_d
    IPR035994 Nucleoside_phosphorylase_sf
    PANTHERiPTHR42679 PTHR42679, 1 hit
    PfamiView protein in Pfam
    PF01048 PNP_UDP_1, 1 hit
    SUPFAMiSSF53167 SSF53167, 1 hit
    TIGRFAMsiTIGR01694 MTAP, 1 hit

    Sequencei

    Sequence statusi: Complete.

    A0QR54-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MMLGVIGGSG FYTFFGSDAR AVSVETPYGP PSAPITVGTV GDHEVAFLPR
    60 70 80 90 100
    HGVKHEFSPH TVPYRANLWA LRSLGVRRVF APCAVGSLTP DLGPGSIVVP
    110 120 130 140 150
    DQLVDRTSGR DDTYFDSGGI HVAFADPYCP TLRAAATGLP GVVDGGTMVV
    160 170 180 190 200
    IQGPRFSTRA ESRWFASQGF TLVNMTGYPE AVLARELEMC YAAVALVTDL
    210 220 230 240 250
    DAGIEVGSGV RAVDVFAEFE RNMPPFKKLV FEALEAVEVE RTCTHCLTHS

    GVQLPFELP
    Length:259
    Mass (Da):27,723
    Last modified:January 9, 2007 - v1
    Checksum:i8D379609536F84FF
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK70512.1
    CP001663 Genomic DNA Translation: AFP37443.1
    RefSeqiWP_011727338.1, NC_008596.1
    YP_885392.1, NC_008596.1

    Genome annotation databases

    EnsemblBacteriaiABK70512; ABK70512; MSMEG_0990
    AFP37443; AFP37443; MSMEI_0963
    GeneIDi4532080
    KEGGimsg:MSMEI_0963
    msm:MSMEG_0990
    PATRICifig|246196.19.peg.978

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK70512.1
    CP001663 Genomic DNA Translation: AFP37443.1
    RefSeqiWP_011727338.1, NC_008596.1
    YP_885392.1, NC_008596.1

    3D structure databases

    ProteinModelPortaliA0QR54
    SMRiA0QR54
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi246196.MSMEG_0990

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABK70512; ABK70512; MSMEG_0990
    AFP37443; AFP37443; MSMEI_0963
    GeneIDi4532080
    KEGGimsg:MSMEI_0963
    msm:MSMEG_0990
    PATRICifig|246196.19.peg.978

    Phylogenomic databases

    eggNOGiENOG4107USC Bacteria
    COG0005 LUCA
    HOGENOMiHOG000228987
    KOiK00772
    OMAiNLYRSWG
    OrthoDBiPOG091H0228

    Enzyme and pathway databases

    UniPathwayi
    UPA00904;UER00873

    Family and domain databases

    HAMAPiMF_01963 MTAP, 1 hit
    InterProiView protein in InterPro
    IPR010044 MTAP
    IPR000845 Nucleoside_phosphorylase_d
    IPR035994 Nucleoside_phosphorylase_sf
    PANTHERiPTHR42679 PTHR42679, 1 hit
    PfamiView protein in Pfam
    PF01048 PNP_UDP_1, 1 hit
    SUPFAMiSSF53167 SSF53167, 1 hit
    TIGRFAMsiTIGR01694 MTAP, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMTAP_MYCS2
    AccessioniPrimary (citable) accession number: A0QR54
    Secondary accession number(s): I7FF12
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 9, 2007
    Last modified: October 10, 2018
    This is version 77 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
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