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UniProtKB - A0Q7Q2 (CS12A_FRATN)
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>sp|A0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a OS=Francisella tularensis subsp. novicida (strain U112) OX=401614 GN=cas12a PE=1 SV=1 MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQIIDKYHQF FIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFK NLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDEALEIIKSFKGWTTYFK GFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAE ELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGI NEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTMQSFYEQIA AFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDDYSVIGTAVLEY ITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLALEEFNKHRDIDKQCRFEEILA NFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQASAEDDVKAIKDLLDQTNNLLHKL KIFHISQSEDKANILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNF ENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYK LLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIEDCRKF IDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISESYIDSVVNQ GKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKK ITHPAKEAIANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEI NLLLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAI EKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFEDLNFGFKRGRFKVE KQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAG FTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKG KWTIASFGSRLINFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESD KKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQDADANGAY HIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRNNCommunity curation ()Add a publicationFeedback
Protein
CRISPR-associated endonuclease Cas12a
Gene
cas12a
Organism
Francisella tularensis subsp. novicida (strain U112)
Status
Reviewed-Annotation score:
Annotation score:5 out of 5
<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>Select a section on the left to see content.
<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Has endonuclease activity on pre-crRNA and dsDNA, using different active sites. A single-RNA guided endonuclease that is also capable of guiding crRNA processing; correct processing of pre-crRNA requires only this protein and the CRISPR locus (PubMed:26422227, PubMed:27096362).
pre-crRNA processing proceeds by an intramolecular nucleophilic attack on the scissile phosphate by the 2'-OH of the upstream ribonucleotide, the divalent cation (which is bound by the crRNA) is probably required for ordering the crRNA pseudoknot and/or increasing RNA binding (PubMed:28431230).
RNA mutagenesis studies show pre-crRNA cleavage is highly sequence- and structure-specific (PubMed:27096362).
Forms a complex with crRNA and complementary dsDNA, where the crRNA displaces the non-target DNA strand and directs endonucleolytic cleavage of both strands of the DNA (PubMed:26422227, PubMed:27096362, PubMed:28431230).
Cleavage results in staggered 5-base 5' overhangs 14-18 and 21-23 bases downstream of the PAM (protospacer adjacent motif) on the non-target and target strands respectively (PubMed:26422227, PubMed:28431230, PubMed:28562584).
Both target and non-target strand DNA are probably independently cleaved in the same active site (PubMed:28431230, PubMed:28562584).
When this protein is expressed in E.coli it prevents plasmids homologous to the first CRISPR spacer from transforming, formally showing it is responsible for plasmid immunity (PubMed:26422227).
4 Publications<p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More...</a></p> Manual assertion based on experiment ini
- Ref.2"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system."
Zetsche B., Gootenberg J.S., Abudayyeh O.O., Slaymaker I.M., Makarova K.S., Essletzbichler P., Volz S.E., Joung J., van der Oost J., Regev A., Koonin E.V., Zhang F.
Cell 163:759-771(2015) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PLASMID RESISTANCE, FUNCTION IN CRRNA FORMATION, FUNCTION AS AN ENDONUCLEASE, POSSIBLE ACTIVE SITE, COFACTOR, POSSIBLE SUBUNIT, MUTAGENESIS OF ASP-917; GLU-1006 AND ASP-1255. - Ref.4"The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA."
Fonfara I., Richter H., Bratovic M., Le Rhun A., Charpentier E.
Nature 532:517-521(2016) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDORIBONUCLEASE, FUNCTION AS AN ENDONUCLEASE, COFACTOR, SUBUNIT, MUTAGENESIS OF HIS-843; LYS-852; LYS-869; PHE-873; ASP-917; GLU-920; HIS-922; TYR-925; GLU-1006; TYR-1024; GLU-1028; ASP-1227 AND ASP-1255, DNA-BINDING, RNA-BINDING. - Ref.8"Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a."
Swarts D.C., van der Oost J., Jinek M.
Mol. Cell 66:221-233(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-1300 IN COMPLEX WITH GUIDE RNA WITH AND WITHOUT TARGET DNA, FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDONUCLEASE, ACTIVE SITE, CATALYTIC ACTIVITY, REACTION MECHANISM, COFACTOR, SUBUNIT, DOMAIN, MUTAGENESIS OF ARG-692; 689-THR--SER-702; GLN-704; ASP-917; GLU-1006; ARG-1218 AND ASP-1255, DNA-BINDING, RNA-BINDING. - Ref.9"Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage."
Stella S., Alcon P., Montoya G.
Nature 546:559-563(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN COMPLEX WITH GUIDE RNA AND PRODUCT DNA, FUNCTION AS AN ENDONUCLEASE, COFACTOR, DOMAIN, MUTAGENESIS OF GLY-608; PRO-663; ASN-666; LYS-667; LYS-671; LYS-677; ARG-692; HIS-694; GLU-1006; 1065-LYS-LYS-1066 AND ARG-1218, DNA-BINDING, RNA-BINDING.
Miscellaneous
Part of a type V-A CRISPR-Cas system.2 Publications
<p>Manually curated information which has been inferred by a curator based on his/her scientific knowledge or on the scientific content of an article.</p> <p><a href="/manual/evidences#ECO:0000305">More...</a></p> Manual assertion inferred by curator fromi
- Ref.3"Discovery and functional characterization of diverse class 2 CRISPR-Cas systems."
Shmakov S., Abudayyeh O.O., Makarova K.S., Wolf Y.I., Gootenberg J.S., Semenova E., Minakhin L., Joung J., Konermann S., Severinov K., Zhang F., Koonin E.V.
Mol. Cell 60:385-397(2015) [PubMed] [Europe PMC] [Abstract]Cited for: DISCUSSION OF SEQUENCE. - Ref.7"Diversity and evolution of class 2 CRISPR-Cas systems."
Shmakov S., Smargon A., Scott D., Cox D., Pyzocha N., Yan W., Abudayyeh O.O., Gootenberg J.S., Makarova K.S., Wolf Y.I., Severinov K., Zhang F., Koonin E.V.
Nat. Rev. Microbiol. 15:169-182(2017) [PubMed] [Europe PMC] [Abstract]Cited for: NOMENCLATURE.
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi
- Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.1 Publication
Manual assertion based on experiment ini
- Ref.8"Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a."
Swarts D.C., van der Oost J., Jinek M.
Mol. Cell 66:221-233(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-1300 IN COMPLEX WITH GUIDE RNA WITH AND WITHOUT TARGET DNA, FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDONUCLEASE, ACTIVE SITE, CATALYTIC ACTIVITY, REACTION MECHANISM, COFACTOR, SUBUNIT, DOMAIN, MUTAGENESIS OF ARG-692; 689-THR--SER-702; GLN-704; ASP-917; GLU-1006; ARG-1218 AND ASP-1255, DNA-BINDING, RNA-BINDING.
- RNA = a 5'-hydroxy-ribonucleotide + n nucleoside-2',3'-cyclophosphates.1 Publication
Manual assertion based on experiment ini
- Ref.8"Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a."
Swarts D.C., van der Oost J., Jinek M.
Mol. Cell 66:221-233(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-1300 IN COMPLEX WITH GUIDE RNA WITH AND WITHOUT TARGET DNA, FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDONUCLEASE, ACTIVE SITE, CATALYTIC ACTIVITY, REACTION MECHANISM, COFACTOR, SUBUNIT, DOMAIN, MUTAGENESIS OF ARG-692; 689-THR--SER-702; GLN-704; ASP-917; GLU-1006; ARG-1218 AND ASP-1255, DNA-BINDING, RNA-BINDING.
<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori
Ca2+
- Search proteins in UniProtKB for this molecule.
- Search chemical reactions in Rhea for this molecule.
- See the description of this molecule in ChEBI.
Manual assertion based on experiment ini
- Ref.3"Discovery and functional characterization of diverse class 2 CRISPR-Cas systems."
Shmakov S., Abudayyeh O.O., Makarova K.S., Wolf Y.I., Gootenberg J.S., Semenova E., Minakhin L., Joung J., Konermann S., Severinov K., Zhang F., Koonin E.V.
Mol. Cell 60:385-397(2015) [PubMed] [Europe PMC] [Abstract]Cited for: DISCUSSION OF SEQUENCE.
- Search proteins in UniProtKB for this molecule.
- Search chemical reactions in Rhea for this molecule.
- See the description of this molecule in ChEBI.
Manual assertion based on experiment ini
- Ref.2"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system."
Zetsche B., Gootenberg J.S., Abudayyeh O.O., Slaymaker I.M., Makarova K.S., Essletzbichler P., Volz S.E., Joung J., van der Oost J., Regev A., Koonin E.V., Zhang F.
Cell 163:759-771(2015) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PLASMID RESISTANCE, FUNCTION IN CRRNA FORMATION, FUNCTION AS AN ENDONUCLEASE, POSSIBLE ACTIVE SITE, COFACTOR, POSSIBLE SUBUNIT, MUTAGENESIS OF ASP-917; GLU-1006 AND ASP-1255. - Ref.3"Discovery and functional characterization of diverse class 2 CRISPR-Cas systems."
Shmakov S., Abudayyeh O.O., Makarova K.S., Wolf Y.I., Gootenberg J.S., Semenova E., Minakhin L., Joung J., Konermann S., Severinov K., Zhang F., Koonin E.V.
Mol. Cell 60:385-397(2015) [PubMed] [Europe PMC] [Abstract]Cited for: DISCUSSION OF SEQUENCE. - Ref.4"The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA."
Fonfara I., Richter H., Bratovic M., Le Rhun A., Charpentier E.
Nature 532:517-521(2016) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDORIBONUCLEASE, FUNCTION AS AN ENDONUCLEASE, COFACTOR, SUBUNIT, MUTAGENESIS OF HIS-843; LYS-852; LYS-869; PHE-873; ASP-917; GLU-920; HIS-922; TYR-925; GLU-1006; TYR-1024; GLU-1028; ASP-1227 AND ASP-1255, DNA-BINDING, RNA-BINDING. - Ref.9"Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage."
Stella S., Alcon P., Montoya G.
Nature 546:559-563(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN COMPLEX WITH GUIDE RNA AND PRODUCT DNA, FUNCTION AS AN ENDONUCLEASE, COFACTOR, DOMAIN, MUTAGENESIS OF GLY-608; PRO-663; ASN-666; LYS-667; LYS-671; LYS-677; ARG-692; HIS-694; GLU-1006; 1065-LYS-LYS-1066 AND ARG-1218, DNA-BINDING, RNA-BINDING.
Manual assertion based on experiment ini
- Ref.2"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system."
Zetsche B., Gootenberg J.S., Abudayyeh O.O., Slaymaker I.M., Makarova K.S., Essletzbichler P., Volz S.E., Joung J., van der Oost J., Regev A., Koonin E.V., Zhang F.
Cell 163:759-771(2015) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PLASMID RESISTANCE, FUNCTION IN CRRNA FORMATION, FUNCTION AS AN ENDONUCLEASE, POSSIBLE ACTIVE SITE, COFACTOR, POSSIBLE SUBUNIT, MUTAGENESIS OF ASP-917; GLU-1006 AND ASP-1255. - Ref.3"Discovery and functional characterization of diverse class 2 CRISPR-Cas systems."
Shmakov S., Abudayyeh O.O., Makarova K.S., Wolf Y.I., Gootenberg J.S., Semenova E., Minakhin L., Joung J., Konermann S., Severinov K., Zhang F., Koonin E.V.
Mol. Cell 60:385-397(2015) [PubMed] [Europe PMC] [Abstract]Cited for: DISCUSSION OF SEQUENCE. - Ref.4"The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA."
Fonfara I., Richter H., Bratovic M., Le Rhun A., Charpentier E.
Nature 532:517-521(2016) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDORIBONUCLEASE, FUNCTION AS AN ENDONUCLEASE, COFACTOR, SUBUNIT, MUTAGENESIS OF HIS-843; LYS-852; LYS-869; PHE-873; ASP-917; GLU-920; HIS-922; TYR-925; GLU-1006; TYR-1024; GLU-1028; ASP-1227 AND ASP-1255, DNA-BINDING, RNA-BINDING. - Ref.8"Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a."
Swarts D.C., van der Oost J., Jinek M.
Mol. Cell 66:221-233(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-1300 IN COMPLEX WITH GUIDE RNA WITH AND WITHOUT TARGET DNA, FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDONUCLEASE, ACTIVE SITE, CATALYTIC ACTIVITY, REACTION MECHANISM, COFACTOR, SUBUNIT, DOMAIN, MUTAGENESIS OF ARG-692; 689-THR--SER-702; GLN-704; ASP-917; GLU-1006; ARG-1218 AND ASP-1255, DNA-BINDING, RNA-BINDING. - Ref.9"Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage."
Stella S., Alcon P., Montoya G.
Nature 546:559-563(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN COMPLEX WITH GUIDE RNA AND PRODUCT DNA, FUNCTION AS AN ENDONUCLEASE, COFACTOR, DOMAIN, MUTAGENESIS OF GLY-608; PRO-663; ASN-666; LYS-667; LYS-671; LYS-677; ARG-692; HIS-694; GLU-1006; 1065-LYS-LYS-1066 AND ARG-1218, DNA-BINDING, RNA-BINDING.
Sites
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| <p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei | 16 | Binds crRNA alone and in crRNA-target DNA heteroduplex2 Publications Manual assertion based on experiment ini
| 1 | |
| Sitei | 131 | Binds target strand DNA1 Publication Manual assertion based on experiment ini
| 1 | |
| Sitei | 295 | Binds crRNA in crRNA-target DNA heteroduplex1 Publication Manual assertion based on experiment ini
| 1 | |
| Sitei | 320 | Binds DNA in crRNA-target DNA heteroduplex1 Publication Manual assertion based on experiment ini
| 1 | |
| Sitei | 334 | Binds DNA in crRNA-target DNA heteroduplex1 Publication Manual assertion based on experiment ini
| 1 | |
| Sitei | 410 | Caps the crRNA-target DNA heteroduplex1 Publication Manual assertion inferred by curator fromi
| 1 | |
| Sitei | 589 | Binds DNA in crRNA-target DNA heteroduplex1 Publication Manual assertion based on experiment ini
| 1 | |
| Sitei | 613 | Binds DNA protospacer adjacent motif (PAM)2 Publications Manual assertion based on experiment ini
| 1 | |
| Sitei | 667 | Binds Target strand DNA1 Publication Manual assertion based on experiment ini
| 1 | |
| Sitei | 671 | Binds PAM2 Publications Manual assertion based on experiment ini
| 1 | |
| Sitei | 677 | Binds Target strand DNA2 Publications Manual assertion based on experiment ini
| 1 | |
| Sitei | 823 | Binds Target strand DNA2 Publications Manual assertion based on experiment ini
| 1 | |
| Sitei | 826 | Binds Target strand DNA; via amide nitrogen2 Publications Manual assertion based on experiment ini
| 1 | |
| Sitei | 833 | Binds crRNA1 Publication Manual assertion based on experiment ini
| 1 | |
| <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei | 843 | For pre-crRNA processing1 Publication Manual assertion inferred by curator fromi
| 1 | |
| Active sitei | 852 | For pre-crRNA processing1 Publication Manual assertion inferred by curator fromi
| 1 | |
| Sitei | 852 | Stabilizes transition state for pre-crRNA processing1 Publication Manual assertion inferred by curator fromi
| 1 | |
| Active sitei | 869 | For pre-crRNA processing1 Publication Manual assertion inferred by curator fromi
| 1 | |
| Active sitei | 917 | For DNase activity of RuvC domain1 Publication Manual assertion inferred by curator fromi
| 1 | |
| Active sitei | 1006 | For DNase activity of RuvC domain1 Publication Manual assertion inferred by curator fromi
| 1 | |
| Sitei | 1026 | Binds DNA in crRNA-target DNA heteroduplex2 Publications Manual assertion based on experiment ini
| 1 | |
| Sitei | 1063 | Binds DNA in crRNA-target DNA heteroduplex2 Publications Manual assertion based on experiment ini
| 1 | |
| Active sitei | 1255 | For DNase activity of RuvC domain1 Publication Manual assertion inferred by curator fromi
| 1 |
<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni
- Bacillus subtilis ribonuclease activity Source: UniProtKB-EC
- deoxyribonuclease I activity Source: UniProtKB-EC
- DNA binding Source: UniProtKB-KW
- lyase activity Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi
| Molecular function | DNA-binding, Endonuclease, Hydrolase, Lyase, Nuclease, RNA-binding |
| Biological process | Antiviral defense |
| Ligand | Calcium, Magnesium |
Enzyme and pathway databases
BioCyc Collection of Pathway/Genome Databases More...BioCyci | FTUL401614:G1G75-1444-MONOMER |
<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi
| <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi | Recommended name: CRISPR-associated endonuclease Cas12a1 Publication<p>Manually curated information that is based on statements in scientific articles for which there is no experimental support.</p> <p><a href="/manual/evidences#ECO:0000303">More...</a></p> Manual assertion based on opinion ini
Manual assertion based on experiment ini
Manual assertion based on experiment ini
Alternative name(s): CRISPR-associated endonuclease Cpf11 Publication Manual assertion based on opinion ini
FnCas12a1 Publication Manual assertion based on opinion ini
FnCpf11 Publication Manual assertion based on opinion ini
|
| <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi | Name:cas12a1 Publication Manual assertion based on opinion ini
Synonyms:cpf11 Publication Manual assertion based on opinion ini
Ordered Locus Names:FTN_1397 |
| <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>Organismi | Francisella tularensis subsp. novicida (strain U112) |
| <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri | 401614 [NCBI] |
| <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineagei | cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella novicida |
| <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi |
|
<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi
<p>This subsection of the 'Pathology and Biotech' section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei
This class of CRISPR enzymes recognize a 5' T-rich protospacer adjacent motif (PAM, TTN for this specific enzyme), unlike Cas9 enzymes which recognize 3' G-rich PAMs, thus this enzyme increases the possibilites for genome editing (PubMed:26422227). The simplicity of the Cas12a-crRNA directed DNA endonuclease activity has been used to target and modify DNA sequences in rice and tobacco (PubMed:27905529).1 Publication
Manual assertion inferred by curator fromi
- Ref.2"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system."
Zetsche B., Gootenberg J.S., Abudayyeh O.O., Slaymaker I.M., Makarova K.S., Essletzbichler P., Volz S.E., Joung J., van der Oost J., Regev A., Koonin E.V., Zhang F.
Cell 163:759-771(2015) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PLASMID RESISTANCE, FUNCTION IN CRRNA FORMATION, FUNCTION AS AN ENDONUCLEASE, POSSIBLE ACTIVE SITE, COFACTOR, POSSIBLE SUBUNIT, MUTAGENESIS OF ASP-917; GLU-1006 AND ASP-1255.
Manual assertion based on experiment ini
- Ref.5"Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida."
Endo A., Masafumi M., Kaya H., Toki S.
Sci. Rep. 6:38169-38169(2016) [PubMed] [Europe PMC] [Abstract]Cited for: BIOTECHNOLOGY. - Ref.6"Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells."
Kim D., Kim J., Hur J.K., Been K.W., Yoon S.H., Kim J.S.
Nat. Biotechnol. 34:863-868(2016) [PubMed] [Europe PMC] [Abstract]Cited for: BIOTECHNOLOGY.
Mutagenesis
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi | 608 | G → A or E: 15% DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 663 | P → A: 25% DNA cleavage, altered non-target strand cleavage products. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 666 | N → A: 80% DNA cleavage, altered non-target strand cleavage products. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 667 | K → A: 30% DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 671 | K → A: 15% DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 677 | K → A: 35% DNA cleavage, altered non-target strand cleavage products. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 692 | R → A: Slight decrease in target DNA cleavage, 30% DNA cleavage, altered non-target strand cleavage products. 2 Publications Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 694 | H → A: Wild-type DNA cleavage, altered non-target strand cleavage products. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 698 – 702 | TKNGS → AGGGG: Loss of target DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 5 | |
| Mutagenesisi | 704 | Q → A: Significant decrease in target DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 843 | H → A: Decreased pre-crRNA processing in vitro, binds RNA, no change in DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 852 | K → A: Decreased pre-crRNA processing in vitro, binds RNA, no change in DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 869 | K → A: Decreased pre-crRNA processing in vitro, binds RNA, no change in DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 873 | F → A: Decreased pre-crRNA processing in vitro, no pre-crRNA processing in E.coli, binds RNA, no change in DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 917 | D → A: Loss of target and non-target strand DNA cleavage, no change in DNA-binding or pre-crRNA processing. 3 Publications Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 920 | E → A: No longer cleaves DNA in presence of Ca(2+). 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 922 | H → A: Decreased cleavage of target strand in presence of Ca(2+), wild-type cleavage of DNA in presence of Mg(2+). 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 925 | Y → A: Decreased cleavage of target strand in presence of Ca(2+), wild-type cleavage of DNA in presence of Mg(2+). 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1006 | E → A: Loss of target and non-target strand DNA cleavage, no change in DNA-binding or pre-crRNA processing. 4 Publications Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1006 | E → Q: Complete loss of DNA cleavage, still binds crRNA; when associated with A-1218. 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1024 | Y → A: No longer cleaves DNA in presence of Ca(2+). 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1028 | E → A: No longer cleaves DNA in presence of Ca(2+), reduced cleavage of non-target strand in presence of Mg(2+). 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1065 – 1066 | KK → AA: 67% DNA cleavage, altered non-target strand cleavage products. 1 Publication Manual assertion based on experiment ini
| 2 | |
| Mutagenesisi | 1218 | R → A: Cleaves both target and non-target strand DNA. Complete loss of DNA cleavage, still binds crRNA; when associated with Q-1006. 2 Publications Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1227 | D → A: No longer cleaves DNA in presence of Ca(2+). 1 Publication Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1255 | D → A: Significant reduction to loss of target and non-target strand DNA cleavage, no change in DNA-binding or pre-crRNA processing. 3 Publications Manual assertion based on experiment ini
| 1 | |
| Mutagenesisi | 1255 | D → N: Significant reduction of target and non-target strand DNA cleavage. 1 Publication Manual assertion based on experiment ini
| 1 |
<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi
Molecule processing
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_0000434902 | 1 – 1300 | CRISPR-associated endonuclease Cas12aAdd BLAST | 1300 |
Proteomic databases
PRoteomics IDEntifications database More...PRIDEi | A0Q7Q2 |
<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni
<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei
Might be a homodimer (PubMed:26422227). Might be a monomer (PubMed:27096362, PubMed:28431230).
3 PublicationsManual assertion based on experiment ini
- Ref.2"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system."
Zetsche B., Gootenberg J.S., Abudayyeh O.O., Slaymaker I.M., Makarova K.S., Essletzbichler P., Volz S.E., Joung J., van der Oost J., Regev A., Koonin E.V., Zhang F.
Cell 163:759-771(2015) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PLASMID RESISTANCE, FUNCTION IN CRRNA FORMATION, FUNCTION AS AN ENDONUCLEASE, POSSIBLE ACTIVE SITE, COFACTOR, POSSIBLE SUBUNIT, MUTAGENESIS OF ASP-917; GLU-1006 AND ASP-1255. - Ref.4"The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA."
Fonfara I., Richter H., Bratovic M., Le Rhun A., Charpentier E.
Nature 532:517-521(2016) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDORIBONUCLEASE, FUNCTION AS AN ENDONUCLEASE, COFACTOR, SUBUNIT, MUTAGENESIS OF HIS-843; LYS-852; LYS-869; PHE-873; ASP-917; GLU-920; HIS-922; TYR-925; GLU-1006; TYR-1024; GLU-1028; ASP-1227 AND ASP-1255, DNA-BINDING, RNA-BINDING. - Ref.8"Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a."
Swarts D.C., van der Oost J., Jinek M.
Mol. Cell 66:221-233(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-1300 IN COMPLEX WITH GUIDE RNA WITH AND WITHOUT TARGET DNA, FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDONUCLEASE, ACTIVE SITE, CATALYTIC ACTIVITY, REACTION MECHANISM, COFACTOR, SUBUNIT, DOMAIN, MUTAGENESIS OF ARG-692; 689-THR--SER-702; GLN-704; ASP-917; GLU-1006; ARG-1218 AND ASP-1255, DNA-BINDING, RNA-BINDING.
<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsHide details| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| <p>This subsection of the <a href="http://www.uniprot.org/help/structure%5Fsection">'Structure'</a> section is used to indicate the positions of experimentally determined hydrogen-bonded turns within the protein sequence. These elements correspond to the DSSP secondary structure code 'T'.<p><a href='/help/turn' target='_top'>More...</a></p>Turni | 3 – 6 | Combined sources <p>Information inferred from a combination of experimental and computational evidence, without manual validation.</p> <p><a href="/manual/evidences#ECO:0000213">More...</a></p> Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| <p>This subsection of the <a href="http://www.uniprot.org/help/structure%5Fsection">'Structure'</a> section is used to indicate the positions of experimentally determined beta strands within the protein sequence.<p><a href='/help/strand' target='_top'>More...</a></p>Beta strandi | 13 – 23 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| <p>This subsection of the <a href="http://www.uniprot.org/help/structure%5Fsection">'Structure'</a> section is used to indicate the positions of experimentally determined helical regions within the protein sequence.<p><a href='/help/helix' target='_top'>More...</a></p>Helixi | 27 – 34 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 8 | |
| Helixi | 36 – 68 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 33 | |
| Helixi | 73 – 86 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 14 | |
| Helixi | 92 – 114 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 23 | |
| Helixi | 117 – 120 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 122 – 124 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 125 – 127 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 132 – 134 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 137 – 147 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Helixi | 153 – 155 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 157 – 159 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 162 – 171 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 10 | |
| Turni | 172 – 174 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 176 – 179 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 180 – 190 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Beta strandi | 192 – 194 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 196 – 198 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 199 – 204 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Helixi | 207 – 224 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 18 | |
| Helixi | 226 – 228 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 231 – 237 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 7 | |
| Turni | 238 – 242 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 5 | |
| Beta strandi | 243 – 245 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Turni | 248 – 251 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 252 – 257 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Helixi | 260 – 263 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 267 – 271 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 5 | |
| Beta strandi | 272 – 274 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 275 – 286 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 12 | |
| Beta strandi | 291 – 293 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 294 – 297 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 300 – 311 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 12 | |
| Helixi | 314 – 319 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Helixi | 345 – 361 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 17 | |
| Beta strandi | 365 – 367 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 370 – 382 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 13 | |
| Helixi | 388 – 390 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 392 – 394 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 397 – 406 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 10 | |
| Helixi | 412 – 422 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Beta strandi | 424 – 426 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 427 – 432 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Helixi | 435 – 445 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Beta strandi | 449 – 452 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 453 – 465 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 13 | |
| Beta strandi | 469 – 471 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 475 – 484 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 10 | |
| Helixi | 487 – 506 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 20 | |
| Beta strandi | 508 – 510 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 513 – 515 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 517 – 519 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 520 – 541 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 22 | |
| Helixi | 559 – 572 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 14 | |
| Helixi | 575 – 586 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 12 | |
| Beta strandi | 595 – 597 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Turni | 603 – 606 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 611 – 613 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 614 – 617 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 619 – 624 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Beta strandi | 627 – 633 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 7 | |
| Beta strandi | 635 – 637 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Turni | 638 – 641 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 643 – 648 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Beta strandi | 650 – 661 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 12 | |
| Helixi | 665 – 673 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 9 | |
| Turni | 676 – 678 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 679 – 682 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 686 – 694 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 9 | |
| Turni | 695 – 697 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 698 – 700 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 704 – 706 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 714 – 730 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 17 | |
| Beta strandi | 731 – 733 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 734 – 737 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 744 – 746 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 750 – 760 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Beta strandi | 761 – 769 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 9 | |
| Helixi | 771 – 779 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 9 | |
| Beta strandi | 782 – 789 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 8 | |
| Helixi | 791 – 793 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 803 – 812 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 10 | |
| Helixi | 814 – 818 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 5 | |
| Beta strandi | 821 – 824 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 829 – 833 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 5 | |
| Beta strandi | 845 – 847 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 852 – 856 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 5 | |
| Beta strandi | 857 – 861 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 5 | |
| Helixi | 871 – 874 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 877 – 887 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Helixi | 888 – 890 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 896 – 906 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Helixi | 908 – 910 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 912 – 917 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Beta strandi | 919 – 921 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 923 – 929 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 7 | |
| Beta strandi | 931 – 933 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 935 – 944 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 10 | |
| Beta strandi | 947 – 952 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Helixi | 953 – 968 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 16 | |
| Turni | 969 – 971 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 977 – 999 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 23 | |
| Beta strandi | 1001 – 1006 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Beta strandi | 1008 – 1011 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 1016 – 1018 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 1019 – 1036 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 18 | |
| Beta strandi | 1041 – 1043 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1045 – 1047 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1050 – 1053 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 1065 – 1067 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1069 – 1071 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1074 – 1077 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 1083 – 1085 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Turni | 1087 – 1089 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 1102 – 1110 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 9 | |
| Beta strandi | 1112 – 1118 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 7 | |
| Turni | 1119 – 1122 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 1123 – 1129 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 7 | |
| Helixi | 1130 – 1132 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1133 – 1135 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1136 – 1138 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1141 – 1145 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 5 | |
| Beta strandi | 1150 – 1153 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 1157 – 1162 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Beta strandi | 1165 – 1168 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 1170 – 1180 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 11 | |
| Helixi | 1192 – 1197 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 6 | |
| Helixi | 1201 – 1214 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 14 | |
| Beta strandi | 1218 – 1220 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Turni | 1222 – 1225 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Beta strandi | 1228 – 1230 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1236 – 1238 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Beta strandi | 1242 – 1245 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 4 | |
| Helixi | 1254 – 1275 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 22 | |
| Beta strandi | 1278 – 1280 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 3 | |
| Helixi | 1288 – 1297 | Combined sources Automatic assertion inferred from combination of experimental and computational evidencei | 10 |
3D structure databases
AlphaFold Protein Structure Database More...AlphaFoldDBi | A0Q7Q2 |
SWISS-MODEL Repository - a database of annotated 3D protein structure models More...SMRi | A0Q7Q2 |
Database of comparative protein structure models More...ModBasei | Search... |
Protein Data Bank in Europe - Knowledge Base More...PDBe-KBi | Search... |
<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi
Region
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni | 1 – 24 | Wedge region 12 Publications Manual assertion based on opinion ini
| 24 | |
| Regioni | 25 – 339 | Recognition domain 12 Publications Manual assertion based on opinion ini
| 315 | |
| Regioni | 47 – 51 | Binds crRNA alone and in crRNA-target DNA heteroduplex2 Publications Manual assertion based on experiment ini
| 5 | |
| Regioni | 182 – 186 | Binds crRNA alone and in crRNA-target DNA heteroduplex2 Publications Manual assertion based on experiment ini
| 5 | |
| Regioni | 301 – 305 | Binds DNA in crRNA-target DNA heteroduplex1 Publication Manual assertion based on experiment ini
| 5 | |
| Regioni | 326 – 329 | Binds crRNA in crRNA-target DNA heteroduplex2 Publications Manual assertion based on experiment ini
| 4 | |
| Regioni | 340 – 591 | Recognition domain 22 Publications Manual assertion based on opinion ini
| 252 | |
| Regioni | 538 – 541 | Binds crRNA in crRNA-target DNA heteroduplex1 Publication Manual assertion based on experiment ini
| 4 | |
| Regioni | 591 – 595 | Binds crRNA1 Publication Manual assertion based on experiment ini
| 5 | |
| Regioni | 592 – 662 | Wedge region 22 Publications Manual assertion based on opinion ini
| 71 | |
| Regioni | 662 – 679 | LKL, important for PAM recognition and DNA unwinding1 Publication Manual assertion inferred by curator fromi
| 18 | |
| Regioni | 663 – 762 | PAM-interacting domain (PI)2 Publications Manual assertion based on opinion ini
| 100 | |
| Regioni | 671 – 677 | Binds DNA protospacer adjacent motif (PAM) on target DNA2 Publications Manual assertion based on experiment ini
| 7 | |
| Regioni | 692 – 704 | Binds single-strand non-target DNA1 Publication Manual assertion based on experiment ini
| 13 | |
| Regioni | 763 – 892 | Wedge region 32 Publications Manual assertion based on opinion ini
| 130 | |
| Regioni | 791 – 794 | Binds crRNA1 Publication Manual assertion based on experiment ini
| 4 | |
| Regioni | 803 – 804 | Binds crRNA1 Publication Manual assertion based on experiment ini
| 2 | |
| Regioni | 851 – 853 | Binds crRNA2 Publications Manual assertion based on experiment ini
| 3 | |
| Regioni | 865 – 873 | Binds crRNA2 Publications Manual assertion based on experiment ini
| 9 | |
| Regioni | 893 – 953 | RuvC-I2 Publications Manual assertion based on opinion ini
| 61 | |
| Regioni | 954 – 971 | Bridge helix2 Publications Manual assertion based on opinion ini
| 18 | |
| Regioni | 972 – 1078 | RuvC-II2 Publications Manual assertion based on opinion ini
| 107 | |
| Regioni | 1079 – 1254 | Nuclease domain2 Publications Manual assertion based on opinion ini
| 176 | |
| Regioni | 1255 – 1300 | RuvC-III2 Publications Manual assertion based on opinion ini
| 46 |
<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini
Has bilobed structure, with the REC lobe (residues 25-591) connected to the NUC lobe (662-1300) by a discontinuous wedge domain (PubMed:28431230, PubMed:28562584). The REC lobe binds the (pre-)crRNA and the crRNA-target DNA heteroduplex (PubMed:28431230, PubMed:28562584). The heteroduplex as well as part of the DNA downstream of the heteroduplex is protected in the central cavity formed by the NUC and REC lobes, which also positions target and non-target DNA for cleavage after domain rearrangement (PubMed:28431230, PubMed:28562584). The LKL region (residues 662 to 679) inserts into target dsDNA initiating its disruption to allow crRNA hybridization, is also involved in determining the non-target strand cleavage site (PubMed:28562584). A 'septum' formed by residues 197-204 and 1061-1070 separates the 2 DNA strands, preventing their reannealing, this region also influences the non-target cleavage site (PubMed:28562584).2 Publications
Manual assertion based on experiment ini
- Ref.8"Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a."
Swarts D.C., van der Oost J., Jinek M.
Mol. Cell 66:221-233(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-1300 IN COMPLEX WITH GUIDE RNA WITH AND WITHOUT TARGET DNA, FUNCTION IN CRRNA PROCESSING, FUNCTION AS AN ENDONUCLEASE, ACTIVE SITE, CATALYTIC ACTIVITY, REACTION MECHANISM, COFACTOR, SUBUNIT, DOMAIN, MUTAGENESIS OF ARG-692; 689-THR--SER-702; GLN-704; ASP-917; GLU-1006; ARG-1218 AND ASP-1255, DNA-BINDING, RNA-BINDING. - Ref.9"Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage."
Stella S., Alcon P., Montoya G.
Nature 546:559-563(2017) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN COMPLEX WITH GUIDE RNA AND PRODUCT DNA, FUNCTION AS AN ENDONUCLEASE, COFACTOR, DOMAIN, MUTAGENESIS OF GLY-608; PRO-663; ASN-666; LYS-667; LYS-671; LYS-677; ARG-692; HIS-694; GLU-1006; 1065-LYS-LYS-1066 AND ARG-1218, DNA-BINDING, RNA-BINDING.
<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi
Belongs to the CRISPR-associated endonuclease Cas12a family.1 Publication
Manual assertion inferred by curator fromi
- Ref.7"Diversity and evolution of class 2 CRISPR-Cas systems."
Shmakov S., Smargon A., Scott D., Cox D., Pyzocha N., Yan W., Abudayyeh O.O., Gootenberg J.S., Makarova K.S., Wolf Y.I., Severinov K., Zhang F., Koonin E.V.
Nat. Rev. Microbiol. 15:169-182(2017) [PubMed] [Europe PMC] [Abstract]Cited for: NOMENCLATURE.
Phylogenomic databases
Identification of Orthologs from Complete Genome Data More...OMAi | QIYNKDF |
Database of Orthologous Groups More...OrthoDBi | 105944at2 |
Family and domain databases
Integrated resource of protein families, domains and functional sites More...InterProi | View protein in InterPro IPR027620, Cas12a IPR040882, Cas12a_NUC IPR040787, Cas12a_REC1 IPR040852, RuvC_1 |
Pfam protein domain database More...Pfami | View protein in Pfam PF18510, NUC, 1 hit PF18501, REC1, 1 hit PF18516, RuvC_1, 1 hit |
TIGRFAMs; a protein family database More...TIGRFAMsi | TIGR04330, cas_Cpf1, 1 hit |
<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei
<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.
A0Q7Q2-1 [UniParc]FASTAAdd to basketAdded to basket
« Hide
Show »10 20 30 40 50
MSIYQEFVNK YSLSKTLRFE LIPQGKTLEN IKARGLILDD EKRAKDYKKA
60 70 80 90 100
KQIIDKYHQF FIEEILSSVC ISEDLLQNYS DVYFKLKKSD DDNLQKDFKS
110 120 130 140 150
AKDTIKKQIS EYIKDSEKFK NLFNQNLIDA KKGQESDLIL WLKQSKDNGI
160 170 180 190 200
ELFKANSDIT DIDEALEIIK SFKGWTTYFK GFHENRKNVY SSNDIPTSII
210 220 230 240 250
YRIVDDNLPK FLENKAKYES LKDKAPEAIN YEQIKKDLAE ELTFDIDYKT
260 270 280 290 300
SEVNQRVFSL DEVFEIANFN NYLNQSGITK FNTIIGGKFV NGENTKRKGI
310 320 330 340 350
NEYINLYSQQ INDKTLKKYK MSVLFKQILS DTESKSFVID KLEDDSDVVT
360 370 380 390 400
TMQSFYEQIA AFKTVEEKSI KETLSLLFDD LKAQKLDLSK IYFKNDKSLT
410 420 430 440 450
DLSQQVFDDY SVIGTAVLEY ITQQIAPKNL DNPSKKEQEL IAKKTEKAKY
460 470 480 490 500
LSLETIKLAL EEFNKHRDID KQCRFEEILA NFAAIPMIFD EIAQNKDNLA
510 520 530 540 550
QISIKYQNQG KKDLLQASAE DDVKAIKDLL DQTNNLLHKL KIFHISQSED
560 570 580 590 600
KANILDKDEH FYLVFEECYF ELANIVPLYN KIRNYITQKP YSDEKFKLNF
610 620 630 640 650
ENSTLANGWD KNKEPDNTAI LFIKDDKYYL GVMNKKNNKI FDDKAIKENK
660 670 680 690 700
GEGYKKIVYK LLPGANKMLP KVFFSAKSIK FYNPSEDILR IRNHSTHTKN
710 720 730 740 750
GSPQKGYEKF EFNIEDCRKF IDFYKQSISK HPEWKDFGFR FSDTQRYNSI
760 770 780 790 800
DEFYREVENQ GYKLTFENIS ESYIDSVVNQ GKLYLFQIYN KDFSAYSKGR
810 820 830 840 850
PNLHTLYWKA LFDERNLQDV VYKLNGEAEL FYRKQSIPKK ITHPAKEAIA
860 870 880 890 900
NKNKDNPKKE SVFEYDLIKD KRFTEDKFFF HCPITINFKS SGANKFNDEI
910 920 930 940 950
NLLLKEKAND VHILSIDRGE RHLAYYTLVD GKGNIIKQDT FNIIGNDRMK
960 970 980 990 1000
TNYHDKLAAI EKDRDSARKD WKKINNIKEM KEGYLSQVVH EIAKLVIEYN
1010 1020 1030 1040 1050
AIVVFEDLNF GFKRGRFKVE KQVYQKLEKM LIEKLNYLVF KDNEFDKTGG
1060 1070 1080 1090 1100
VLRAYQLTAP FETFKKMGKQ TGIIYYVPAG FTSKICPVTG FVNQLYPKYE
1110 1120 1130 1140 1150
SVSKSQEFFS KFDKICYNLD KGYFEFSFDY KNFGDKAAKG KWTIASFGSR
1160 1170 1180 1190 1200
LINFRNSDKN HNWDTREVYP TKELEKLLKD YSIEYGHGEC IKAAICGESD
1210 1220 1230 1240 1250
KKFFAKLTSV LNTILQMRNS KTGTELDYLI SPVADVNGNF FDSRQAPKNM
1260 1270 1280 1290 1300
PQDADANGAY HIGLKGLMLL GRIKNNQEGK KLNLVIKNEE YFEFVQNRNN
Length:1,300
Mass (Da):151,915
Last modified:January 9, 2007 - v1
<p>The checksum is a form of redundancy check that is calculated
from the sequence. It is useful for tracking sequence updates.</p>
<p>It should be noted that while, in theory, two different sequences could
have the same checksum value, the likelihood that this would happen
is extremely low.</p>
<p>However UniProtKB may contain entries with identical sequences in case
of multiple genes (paralogs).</p>
<p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64)
using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1.
The algorithm is described in the ISO 3309 standard.
</p>
<p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br />
<strong>Cyclic redundancy and other checksums</strong><br />
<a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p>
Checksum:i601E903DE68C80DE
Sequence databases
| Select the link destinations: EMBL nucleotide sequence database More...EMBLiGenBank nucleotide sequence database More...GenBankiDNA Data Bank of Japan; a nucleotide sequence database More...DDBJiLinks Updated | CP000439 Genomic DNA Translation: ABK90267.1 |
NCBI Reference Sequences More...RefSeqi | WP_003040289.1, NZ_CP009633.1 |
Genome annotation databases
Ensembl bacterial and archaeal genome annotation project More...EnsemblBacteriai | ABK90267; ABK90267; FTN_1397 |
KEGG: Kyoto Encyclopedia of Genes and Genomes More...KEGGi | ftn:FTN_1397 |
<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi
| Protein | Similar proteins | Species | Score | Length | Source | |
|---|---|---|---|---|---|---|
| A0Q7Q2 | Type V CRISPR-associated protein Cpf1 | 1300 | UniRef100_A0Q7Q2 | |||
| CRISPR-associated protein Cpf1, subtype PREFRAN | 1300 |
| Protein | Similar proteins | Species | Score | Length | Source | |
|---|---|---|---|---|---|---|
| A0Q7Q2 | Type V CRISPR-associated protein Cpf1 | 1300 | UniRef90_A0Q7Q2 | |||
| CRISPR-associated protein Cpf1, subtype PREFRAN | 1300 | |||||
| Type V CRISPR-associated protein Cpf1 | 1300 | |||||
| +11 | ||||||
| Protein | Similar proteins | Species | Score | Length | Source | |
|---|---|---|---|---|---|---|
| A0Q7Q2 | Type V CRISPR-associated protein Cpf1 | 1300 | UniRef50_A0Q7Q2 | |||
| CRISPR-associated protein Cpf1, subtype PREFRAN | 1300 | |||||
| Type V CRISPR-associated protein Cpf1 | 1300 | |||||
| +11 | ||||||
<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi
Sequence databases
| Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000439 Genomic DNA Translation: ABK90267.1 |
| RefSeqi | WP_003040289.1, NZ_CP009633.1 |
3D structure databases
| Select the link destinations: Protein Data Bank Europe More...PDBeiProtein Data Bank RCSB More...RCSB PDBiProtein Data Bank Japan More...PDBjiLinks Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
| 5MGA | X-ray | 3.00 | A | 1-1300 | [»] | |
| 5NFV | X-ray | 2.50 | A | 2-1300 | [»] | |
| 5NG6 | X-ray | 3.34 | A/C/E/G | 2-1300 | [»] | |
| 6GTC | electron microscopy | 3.91 | A | 1-1300 | [»] | |
| 6GTD | electron microscopy | 4.24 | A | 1-1300 | [»] | |
| 6GTE | electron microscopy | 4.07 | A | 1-1300 | [»] | |
| 6GTF | electron microscopy | 3.63 | A | 1-1300 | [»] | |
| 6GTG | electron microscopy | 3.27 | A | 1-1300 | [»] | |
| 6I1K | X-ray | 2.65 | A | 2-1300 | [»] | |
| 6I1L | X-ray | 2.98 | A/D | 2-1300 | [»] | |
| AlphaFoldDBi | A0Q7Q2 | |||||
| SMRi | A0Q7Q2 | |||||
| ModBasei | Search... | |||||
| PDBe-KBi | Search... | |||||
Proteomic databases
| PRIDEi | A0Q7Q2 |
Genome annotation databases
| EnsemblBacteriai | ABK90267; ABK90267; FTN_1397 |
| KEGGi | ftn:FTN_1397 |
Phylogenomic databases
| OMAi | QIYNKDF |
| OrthoDBi | 105944at2 |
Enzyme and pathway databases
| BioCyci | FTUL401614:G1G75-1444-MONOMER |
Family and domain databases
| InterProi | View protein in InterPro IPR027620, Cas12a IPR040882, Cas12a_NUC IPR040787, Cas12a_REC1 IPR040852, RuvC_1 |
| Pfami | View protein in Pfam PF18510, NUC, 1 hit PF18501, REC1, 1 hit PF18516, RuvC_1, 1 hit |
| TIGRFAMsi | TIGR04330, cas_Cpf1, 1 hit |
MobiDB: a database of protein disorder and mobility annotations More...MobiDBi | Search... |
<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi
| <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry namei | CS12A_FRATN | |
| <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>Accessioni | A0Q7Q2Primary (citable) accession number: A0Q7Q2 | |
| <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 9, 2015 |
| Last sequence update: | January 9, 2007 | |
| Last modified: | May 25, 2022 | |
| This is version 67 of the entry and version 1 of the sequence. See complete history. | ||
| <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
| Annotation program | Prokaryotic Protein Annotation Program | |
<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families
