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UniProtKB - A0PPJ8 (METK_MYCUA)
Protein
S-adenosylmethionine synthase
Gene
metK
Organism
Mycobacterium ulcerans (strain Agy99)
Status
Functioni
Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.
UniRule annotationCatalytic activityi
- EC:2.5.1.6UniRule annotation
Cofactori
Protein has several cofactor binding sites:- Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation
- K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation
: S-adenosyl-L-methionine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 17 | ATPUniRule annotation | 1 | |
Metal bindingi | 19 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 45 | PotassiumUniRule annotation | 1 | |
Binding sitei | 58 | MethionineUniRule annotation | 1 | |
Binding sitei | 104 | MethionineUniRule annotation | 1 | |
Binding sitei | 259 | ATP; shared with neighboring subunitUniRule annotation | 1 | |
Binding sitei | 259 | Methionine; shared with neighboring subunitUniRule annotation | 1 | |
Binding sitei | 282 | ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation | 1 | |
Binding sitei | 286 | ATP; shared with neighboring subunitUniRule annotation | 1 | |
Binding sitei | 290 | MethionineUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 179 – 181 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 250 – 251 | ATPUniRule annotation | 2 | |
Nucleotide bindingi | 265 – 266 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- methionine adenosyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- one-carbon metabolic process Source: UniProtKB-UniRule
- S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | One-carbon metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
UniPathwayi | UPA00315;UER00080 |
Names & Taxonomyi
Protein namesi | Recommended name: S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)Short name: AdoMet synthaseUniRule annotation Alternative name(s): MATUniRule annotation Methionine adenosyltransferaseUniRule annotation |
Gene namesi | Name:metKUniRule annotation Ordered Locus Names:MUL_1791 |
Organismi | Mycobacterium ulcerans (strain Agy99) |
Taxonomic identifieri | 362242 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000302946 | 1 – 403 | S-adenosylmethionine synthaseAdd BLAST | 403 |
Interactioni
Subunit structurei
Homotetramer; dimer of dimers.
UniRule annotationProtein-protein interaction databases
STRINGi | 362242.MUL_1791 |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 104 – 114 | Flexible loopUniRule annotationAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the AdoMet synthase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0192, Bacteria |
HOGENOMi | CLU_041802_1_1_11 |
OMAi | INPTGRY |
Family and domain databases
HAMAPi | MF_00086, S_AdoMet_synth1, 1 hit |
InterProi | View protein in InterPro IPR022631, ADOMET_SYNTHASE_CS IPR022630, S-AdoMet_synt_C IPR022629, S-AdoMet_synt_central IPR022628, S-AdoMet_synt_N IPR002133, S-AdoMet_synthetase IPR022636, S-AdoMet_synthetase_sfam |
PANTHERi | PTHR11964, PTHR11964, 1 hit |
Pfami | View protein in Pfam PF02773, S-AdoMet_synt_C, 1 hit PF02772, S-AdoMet_synt_M, 1 hit PF00438, S-AdoMet_synt_N, 1 hit |
PIRSFi | PIRSF000497, MAT, 1 hit |
SUPFAMi | SSF55973, SSF55973, 3 hits |
TIGRFAMsi | TIGR01034, metK, 1 hit |
PROSITEi | View protein in PROSITE PS00376, ADOMET_SYNTHASE_1, 1 hit PS00377, ADOMET_SYNTHASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
A0PPJ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEKGRLFTS ESVTEGHPDK ICDAVSDSVL DALLAADPRS RVAVETLVTT
60 70 80 90 100
GQVHVVGEVT TTAKEAFADI TNIVRERILD IGYDSSDKGF DGASCGVNIG
110 120 130 140 150
IGAQSPDIAQ GVDTAHEARV EGAADPLDAQ GAGDQGLMFG YAINDTPELM
160 170 180 190 200
PLPIALAHRL SRRLTEVRKN GVLPYLRPDG KTQVTIAYED RVPVRLDTVV
210 220 230 240 250
ISTQHADDID LVKTLDPDIR EQVLKTVLDD LAHDTLDASA VRVLVNPTGK
260 270 280 290 300
FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR
310 320 330 340 350
WVAKNVVAAG LAEGVEVQVA YAIGKAAPVG LFVETFGSEA VDPVKIEKAI
360 370 380 390 400
GEVFDLRPGA IIRDLNLLRP IYAPTAAYGH FGRTDVDLPW ERLDKVDDLK
RAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000325 Genomic DNA Translation: ABL04267.1 |
RefSeqi | WP_011739887.1, NC_008611.1 |
Genome annotation databases
EnsemblBacteriai | ABL04267; ABL04267; MUL_1791 |
KEGGi | mul:MUL_1791 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000325 Genomic DNA Translation: ABL04267.1 |
RefSeqi | WP_011739887.1, NC_008611.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 362242.MUL_1791 |
Genome annotation databases
EnsemblBacteriai | ABL04267; ABL04267; MUL_1791 |
KEGGi | mul:MUL_1791 |
Phylogenomic databases
eggNOGi | COG0192, Bacteria |
HOGENOMi | CLU_041802_1_1_11 |
OMAi | INPTGRY |
Enzyme and pathway databases
UniPathwayi | UPA00315;UER00080 |
Family and domain databases
HAMAPi | MF_00086, S_AdoMet_synth1, 1 hit |
InterProi | View protein in InterPro IPR022631, ADOMET_SYNTHASE_CS IPR022630, S-AdoMet_synt_C IPR022629, S-AdoMet_synt_central IPR022628, S-AdoMet_synt_N IPR002133, S-AdoMet_synthetase IPR022636, S-AdoMet_synthetase_sfam |
PANTHERi | PTHR11964, PTHR11964, 1 hit |
Pfami | View protein in Pfam PF02773, S-AdoMet_synt_C, 1 hit PF02772, S-AdoMet_synt_M, 1 hit PF00438, S-AdoMet_synt_N, 1 hit |
PIRSFi | PIRSF000497, MAT, 1 hit |
SUPFAMi | SSF55973, SSF55973, 3 hits |
TIGRFAMsi | TIGR01034, metK, 1 hit |
PROSITEi | View protein in PROSITE PS00376, ADOMET_SYNTHASE_1, 1 hit PS00377, ADOMET_SYNTHASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | METK_MYCUA | |
Accessioni | A0PPJ8Primary (citable) accession number: A0PPJ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | January 9, 2007 | |
Last modified: | February 23, 2022 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families