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Entry version 46 (05 Dec 2018)
Sequence version 1 (09 Jan 2007)
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Protein

Alpha-amylase

Gene

igtZ

Organism
Bacillus circulans
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts on maltooligosaccharides that have a degree of polymerization (DP) of 4 or more, amylose, and soluble or raw starch to produce glucose and maltooligosaccharides up to DP5 by a hydrolysis reaction. Also acts on maltooligosyl trehaloses that have DP5 or more to produce trehalose as the major hydrolysis product.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication EC:3.2.1.1

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Optimum pH is 6.0.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei231Nucleophile1 Publication1
Active sitei373Proton donor1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
CBM20 Carbohydrate-Binding Module Family 20
CBM25 Carbohydrate-Binding Module Family 25
GH119 Glycoside Hydrolase Family 119

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alpha-amylase1 Publication (EC:3.2.1.11 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:igtZ1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus circulans
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1397 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 311 PublicationAdd BLAST31
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000043222932 – 1290Alpha-amylaseAdd BLAST1259

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
A0P8X0

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini817 – 902Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST86
Domaini1183 – 1289CBM20PROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni902 – 978CBM25Sequence analysisAdd BLAST77
Regioni1092 – 1171CBM25Sequence analysisAdd BLAST80

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi979 – 1089Thr-richPROSITE-ProRule annotationAdd BLAST111
Compositional biasi995 – 1090Pro-richPROSITE-ProRule annotationAdd BLAST96

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00063 FN3, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.40.10, 4 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013784 Carb-bd-like_fold
IPR005085 CBM25
IPR002044 CBM_fam20
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR004300 Glyco_hydro_57_N
IPR013783 Ig-like_fold

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03423 CBM_25, 2 hits
PF00041 fn3, 1 hit
PF03065 Glyco_hydro_57, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01065 CBM_2, 1 hit
SM01066 CBM_25, 2 hits
SM00060 FN3, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF49265 SSF49265, 1 hit
SSF49452 SSF49452, 1 hit
SSF88713 SSF88713, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51166 CBM20, 1 hit
PS50853 FN3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

A0P8X0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTRKTRYLHQ ITTLILGGLL IVPAAAPPVS ADIAATHVYH NHMPNFWAYY
60 70 80 90 100
DLNTYNSTPV GSPIRYTYDG EVIQLKQNPP AGYPYYLPNG SPMPHDDLVS
110 120 130 140 150
YYSHHAKTGA YLTWPWSVAN TLHSSHPQAQ MHVTMSGSVV NNVNSIIQQG
160 170 180 190 200
NVSGYNNPAW GTPWKNAVTQ LKTAGGDNRL DLIHFSGHHS MGPLVGNDYL
210 220 230 240 250
LKDMIYHGAT MAQPYFLGSS YKSSKGFFPT ELGFSERIIP VLNKLGIQWS
260 270 280 290 300
VIGNNHFSRT LKDYPLLDSP GTDTMISPPN RSDLQNVSTA GAWVNEPMFN
310 320 330 340 350
EQQVVYNKYP FASTAHWVRY VDPATGAESR VVGVPVAQAQ SWEEGYLGQV
360 370 380 390 400
KADALKPYEN LVAQKQIFVV AHDGDNSSGR AGSEETWRNA GNVTYADSGV
410 420 430 440 450
TGMGIDEYLR SNTPAAADVV HVQDGSWIDT RDSSSDPAWY HWHLPFGIWK
460 470 480 490 500
GQFAAFNQVN GTAYAPKKNL AGVEEGMTVS FEKGYHYLER NFALLQASLN
510 520 530 540 550
YAKTAEQIWL EEHPNYWKPA NPLDREVTYE GNQLNPWMLS YPVKGNPAND
560 570 580 590 600
YAGGANPAEL AWYFLLPAMD SGFGYYDENV DDSVKPALSF NQSLYFSKPY
610 620 630 640 650
VSQKLAKDKT GPSVWWPQRY PYNPGSANVS KAEGWTLQHY NNAFAIYTYA
660 670 680 690 700
FDTSGISEIK VKVRAHRDKT ADAADNTFKV YDPAGLAAAG IANIDPAKVG
710 720 730 740 750
AWTEYPMNVR DLSADINGVD WQPSSMTIMQ KVPATDIGNL YFSYISDYRD
760 770 780 790 800
QLLDYFIEAK DAKGNVTQSD IQQVYVGAGK YKLANGKYTE SMQGTIEGTH
810 820 830 840 850
PFITDVPAVP DTEAPAVPAN LQATVMNASS VGLSWNAATD NIRVTGYEIY
860 870 880 890 900
RNGVRIGTTP STSYTDSGLS ASTAYEYRVK AYDASGNLSG FSAAATATTP
910 920 930 940 950
AGNHVTVYYK QGYSTPYIHY RPAGGTWTTA PGVAIPAAEV AGYNKITINI
960 970 980 990 1000
GAATQLEACF NNGSGTWDSN GGSNYLFGTG TWTYTPTGKI QAGAPVAPSA
1010 1020 1030 1040 1050
TPTVAPTATP TPKPSVTPTV TPITTPTVAP TLSPTPTVAP TVKPSATPIA
1060 1070 1080 1090 1100
TPTVTPTVSP TATPTVVPTI APTATPTTSP SATPVPTATP AGNSATIYYK
1110 1120 1130 1140 1150
NTAFSNSYIH YKLDGATAWT TSPGVQMQAS TFSGYKAITI PLGSATGLTA
1160 1170 1180 1190 1200
AFNNGSGIWD NNGGSNYHFG TGSSSLTGGN LITGEPQADS VTFRVSVPGS
1210 1220 1230 1240 1250
TPANAPVYLT GSFNSWNAAD PAYLLTRGSD GIYSITLNLP AGSAVTYKLT
1260 1270 1280 1290
RGSWATVETA SSGADITNRT LTPAGGAQTV TLTVQRWKDQ
Length:1,290
Mass (Da):138,787
Last modified:January 9, 2007 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD205B76690E68198
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB248273 Genomic DNA Translation: BAF37284.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB248273 Genomic DNA Translation: BAF37284.1

3D structure databases

ProteinModelPortaliA0P8X0
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
CBM25 Carbohydrate-Binding Module Family 25
GH119 Glycoside Hydrolase Family 119

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 4 hits
InterProiView protein in InterPro
IPR013784 Carb-bd-like_fold
IPR005085 CBM25
IPR002044 CBM_fam20
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR004300 Glyco_hydro_57_N
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF03423 CBM_25, 2 hits
PF00041 fn3, 1 hit
PF03065 Glyco_hydro_57, 1 hit
SMARTiView protein in SMART
SM01065 CBM_2, 1 hit
SM01066 CBM_25, 2 hits
SM00060 FN3, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
SSF49452 SSF49452, 1 hit
SSF88713 SSF88713, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
PS50853 FN3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAAMY_BACCI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0P8X0
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: January 9, 2007
Last modified: December 5, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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