UniProtKB - A0MQH0 (DICER_CRIGR)
Endoribonuclease Dicer
DICER1
Functioni
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity).
By similarityCaution
Catalytic activityi
- Endonucleolytic cleavage to 5'-phosphomonoester. EC:3.1.26.3
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1316 | Magnesium 1By similarity | 1 | |
Metal bindingi | 1395 | Magnesium 1By similarity | 1 | |
Metal bindingi | 1398 | Magnesium 1By similarity | 1 | |
Metal bindingi | 1700 | Magnesium 2By similarity | 1 | |
Sitei | 1801 | Important for activityBy similarity | 1 | |
Metal bindingi | 1805 | Magnesium 2By similarity | 1 | |
Metal bindingi | 1808 | Magnesium 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 64 – 71 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: InterPro
- double-stranded RNA binding Source: Ensembl
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- pre-miRNA binding Source: Ensembl
- protein domain specific binding Source: Ensembl
- ribonuclease III activity Source: UniProtKB-EC
- siRNA binding Source: Ensembl
GO - Biological processi
- miRNA loading onto RISC involved in gene silencing by miRNA Source: Ensembl
- negative regulation of tumor necrosis factor production Source: Ensembl
- NIK/NF-kappaB signaling Source: Ensembl
- pre-miRNA processing Source: UniProtKB
- production of siRNA involved in RNA interference Source: UniProtKB
- siRNA loading onto RISC involved in RNA interference Source: Ensembl
- targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
Keywordsi
Molecular function | Endonuclease, Helicase, Hydrolase, Nuclease, RNA-binding |
Biological process | RNA-mediated gene silencing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DICER1 Synonyms:DICER |
Organismi | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) |
Taxonomic identifieri | 10029 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Cricetidae › Cricetinae › Cricetulus |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Extracellular region or secreted
- extracellular exosome Source: Ensembl
Other locations
- perinuclear region of cytoplasm Source: UniProtKB
- RISC complex Source: UniProtKB
- RISC-loading complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000373983 | 1 – 1917 | Endoribonuclease DicerAdd BLAST | 1917 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 413 | PhosphoserineBy similarity | 1 | |
Modified residuei | 415 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1016 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1160 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1456 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1464 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1466 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1863 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC.
Interacts with DHX9, AGO1, PIWIL1 and PRKRA.
Interacts with AGO2, TARBP2, EIF6, MOV10 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).
Interacts with BCDIN3D (By similarity).
Interacts (via Dicer dsRNA-binding fold domain) with ALOX5 (via PLAT domain); this interaction enhances arachidonate 5-lipoxygenase activity and modifies the miRNA precursor processing activity of DICER1 (By similarity).
By similarityGO - Molecular functioni
- protein domain specific binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 10029.NP_001231198.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 227 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 177 | |
Domaini | 433 – 602 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 170 | |
Domaini | 630 – 722 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 898 – 1042 | PAZPROSITE-ProRule annotationAdd BLAST | 145 | |
Domaini | 1276 – 1403 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 128 | |
Domaini | 1661 – 1819 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 159 | |
Domaini | 1844 – 1909 | DRBMPROSITE-ProRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 256 – 595 | Required for interaction with PRKRA and TARBP2By similarityAdd BLAST | 340 | |
Regioni | 409 – 433 | DisorderedSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 175 – 178 | DECH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 412 – 427 | Acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0701, Eukaryota |
GeneTreei | ENSGT00940000156287 |
OMAi | TTYLYCT |
OrthoDBi | 1337630at2759 |
Family and domain databases
CDDi | cd10843, DSRM_DICER, 1 hit cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR044441, DICER_DSRM IPR014720, dsRBD_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 1 hit SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE
60 70 80 90 100
LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PRAKRTVFLV
110 120 130 140 150
NSANQVAQQV SAVRTHSDLK VGEYSNLEVN ASWTKERWSQ EFTKHQVLIM
160 170 180 190 200
TCYVALNVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCESCPSCP
210 220 230 240 250
RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL VVLDRYASQP
260 270 280 290 300
CEIVVDCGPF TDRSGLYERL LMELEEAINF INDCNVSVHS KERDSTLISK
310 320 330 340 350
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT
360 370 380 390 400
LLRKIHALCE EHFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW
410 420 430 440 450
YNNRNQDNYV SWSDSEDDDD DEEIEEKEKP ETNFPSPFTN ILCGIIFVER
460 470 480 490 500
RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR SKQMEAEFRK
510 520 530 540 550
QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP TEYRSYVQSK
560 570 580 590 600
GRARAPISNY VMLADTDKIQ SFEEDLKTYK AIEKILRNKC SKSVDGAEAD
610 620 630 640 650
VDAVVDDDDV FPPYVLRPDD GGPRVTINTA IGHVNRYCAR LPSDPFTHLA
660 670 680 690 700
PKCRTQELPD GTFYSTLYLP INSPLRASIV GPPMGCVRLA ERVVALICCE
710 720 730 740 750
KLHKIGELDE HLMPVGKETV KYEEELDLHD EEETSVPGRP GSTKRRQCYP
760 770 780 790 800
KAIPECLRES YPKPDQPCYL YVIGMVLTTP LPDELNFRRR KLYPPEDTTR
810 820 830 840 850
CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ QMLELITRLH
860 870 880 890 900
QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSSTL DIDFTFMEDI
910 920 930 940 950
EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV
960 970 980 990 1000
YTDLTPLSKF PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL
1010 1020 1030 1040 1050
TPRHLNQKGK ALPLSSAEKR KAKWESLQNK QILVPELCAI HPIPASLWRK
1060 1070 1080 1090 1100
AVCLPSILYR LHCLLTAEEL RAQTASDAGV GVRSLPADFR YPNLDFGWKK
1110 1120 1130 1140 1150
SIDSKSFIST CNSSLAENDN YCKHSTIIVP ENAAHQGATR TPLENHDQMS
1160 1170 1180 1190 1200
VNCRRLPAES SGKLQIEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN
1210 1220 1230 1240 1250
QLNYFKQEIP VQPTTSYPIQ NLYHYENQPK PSNECTLLSN KYLDGNANTS
1260 1270 1280 1290 1300
TSDGSPAVST MPAVMSAGRA LKDRMDSEQS PSAGYSSRTL GPNPGLILQA
1310 1320 1330 1340 1350
LTLSNASDGF NLERLEMLGD SFLKHAITTY LFCTYPDAHE GRLSYMRSKK
1360 1370 1380 1390 1400
VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW LPPGYVVNQD KSNAEKWEKD
1410 1420 1430 1440 1450
EMTKDCLLAN GKLGKDCEEE EALTWRAPKE EAEYEDDFLE YDQEHIQFID
1460 1470 1480 1490 1500
SMLMGSGAFV KKISLSPFST SDSAYEWKMP KKSSLGSMPF SSDLEDFDYS
1510 1520 1530 1540 1550
SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI
1560 1570 1580 1590 1600
ADKSIADCVE ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALY
1610 1620 1630 1640 1650
PAQENFSSQQ KSLSGSCAAT AASPRSSAGK DLEYGCLKIP PRCMFDHPDA
1660 1670 1680 1690 1700
EKTLNHLISG FENFEKKINY RFKNKAYLLQ AFTHASYHYN TITDCYQRLE
1710 1720 1730 1740 1750
FLGDAILDYL ITKHLYEDPR QHSPGVLTDL RSALVNNTIF ASLAVKYDYH
1760 1770 1780 1790 1800
KYFKAVSPEL FHVIDDFVQF QLEKNEMQGM DSELRRSEED EEKEEDIEVP
1810 1820 1830 1840 1850
KAMGDIFESL AGAIYMDSGM SLEVVWQVYY PMMRPLIEKF SANVPRSPVR
1860 1870 1880 1890 1900
ELLEMEPETA KFSPAERTYD GKVRVTVEVV GKGKFKGVGR SYRIAKSAAA
1910
RRALRSLKAN QPQVPNS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF031271 mRNA Translation: ABK28790.1 |
RefSeqi | NP_001231198.1, NM_001244269.1 XP_007647243.1, XM_007649053.1 |
Genome annotation databases
Ensembli | ENSCGRT00001009453; ENSCGRP00001006065; ENSCGRG00001008088 |
GeneIDi | 100689239 |
KEGGi | cge:100689239 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF031271 mRNA Translation: ABK28790.1 |
RefSeqi | NP_001231198.1, NM_001244269.1 XP_007647243.1, XM_007649053.1 |
3D structure databases
AlphaFoldDBi | A0MQH0 |
SMRi | A0MQH0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10029.NP_001231198.1 |
Genome annotation databases
Ensembli | ENSCGRT00001009453; ENSCGRP00001006065; ENSCGRG00001008088 |
GeneIDi | 100689239 |
KEGGi | cge:100689239 |
Organism-specific databases
CTDi | 23405 |
Phylogenomic databases
eggNOGi | KOG0701, Eukaryota |
GeneTreei | ENSGT00940000156287 |
OMAi | TTYLYCT |
OrthoDBi | 1337630at2759 |
Family and domain databases
CDDi | cd10843, DSRM_DICER, 1 hit cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR044441, DICER_DSRM IPR014720, dsRBD_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 1 hit SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | DICER_CRIGR | |
Accessioni | A0MQH0Primary (citable) accession number: A0MQH0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | September 1, 2009 | |
Last modified: | May 25, 2022 | |
This is version 90 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |