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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Acidothermus cellulolyticus (strain ATCC 43068 / 11B)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi171Zinc 1UniRule annotation1
Metal bindingi174Zinc 1UniRule annotation1
Metal bindingi188Zinc 2UniRule annotation1
Metal bindingi191Zinc 2UniRule annotation1
Metal bindingi210Zinc 2UniRule annotation1
Metal bindingi213Zinc 2UniRule annotation1
Metal bindingi224Zinc 1UniRule annotation1
Metal bindingi227Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 236CR-typePROSITE-ProRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotation, Stress responseUniRule annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:Acel_2117Imported
OrganismiAcidothermus cellulolyticus (strain ATCC 43068 / 11B)Imported
Taxonomic identifieri351607 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaAcidothermalesAcidothermaceaeAcidothermus
Proteomesi
  • UP000008221 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi351607.Acel_2117

Structurei

3D structure databases

ProteinModelPortaliA0LWS9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 83JInterPro annotationAdd BLAST66
Domaini158 – 236CR-typeInterPro annotationAdd BLAST79
Repeati171 – 178CXXCXGXG motifUniRule annotation8
Repeati188 – 195CXXCXGXG motifUniRule annotation8
Repeati210 – 217CXXCXGXG motifUniRule annotation8
Repeati224 – 231CXXCXGXG motifUniRule annotation8

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 236CR-typePROSITE-ProRule annotationAdd BLAST79

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105BZ5 Bacteria
COG0484 LUCA
HOGENOMiHOG000226717
KOiK03686
OMAiKEGMMDH
OrthoDBiPOG091H00PT

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

A0LWS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHAHPTTRMS ARDYIEKDYY KVLGVPKDAS IDEIKKAYRK LARQYHPDAN
60 70 80 90 100
KGDPKAEERF KEISEAYDVL SDEKKRKEYD EARSLFGSGG FRIPTGSGTG
110 120 130 140 150
TTGGFPFDLG DLFGGLRTGG LGDILGGIFG GGSRRAQPRR GADVETEITI
160 170 180 190 200
SFTDAIRGVT VPIRLTSPGP CPSCHGTGAR AGTMPRVCPN CSGTGMVSRV
210 220 230 240 250
VGGFALSEPC RECRGRGLVV DDPCPSCRGS GRATSERTLQ VRIPAGVDDG
260 270 280 290 300
QRIRLKGRGA PGENGGPAGD LIVLVHVQPH KVFGRSGPNL TLTLPVTFPE
310 320 330 340 350
AALGATIEVP TLDGPVTVKL PPGTSSGRVL RVRGKGAPRR DGSRGDLLVT
360 370 380
VEVVVPKKLS SKAREALEAF RSATEDENPR AGLLVGS
Length:387
Mass (Da):40,969
Last modified:December 12, 2006 - v1
Checksum:i1B74B095122DAB8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000481 Genomic DNA Translation: ABK53889.1

Genome annotation databases

EnsemblBacteriaiABK53889; ABK53889; Acel_2117
KEGGiace:Acel_2117

Similar proteinsi

Entry informationi

Entry nameiA0LWS9_ACIC1
AccessioniPrimary (citable) accession number: A0LWS9
Entry historyiIntegrated into UniProtKB/TrEMBL: December 12, 2006
Last sequence update: December 12, 2006
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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