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Protein

Mediator of RNA polymerase II transcription subunit 22

Gene

Med22

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 22
Alternative name(s):
Mediator complex subunit 22
Surfeit locus protein 5
Gene namesi
Name:Med22
Synonyms:Surf5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1564893 Med22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002781221 – 200Mediator of RNA polymerase II transcription subunit 22Add BLAST200

Proteomic databases

PaxDbiA0JPN6
PeptideAtlasiA0JPN6
PRIDEiA0JPN6

Expressioni

Gene expression databases

BgeeiENSRNOG00000005059
GenevisibleiA0JPN6 RN

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062301

Structurei

3D structure databases

SMRiA0JPN6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili93 – 122Sequence analysisAdd BLAST30

Sequence similaritiesi

Belongs to the Mediator complex subunit 22 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3304 Eukaryota
ENOG410XS6U LUCA
GeneTreeiENSGT00390000004339
HOGENOMiHOG000006377
HOVERGENiHBG058487
InParanoidiA0JPN6
KOiK15139
OMAiKQAECDQ
OrthoDBiEOG091G0SIM
PhylomeDBiA0JPN6
TreeFamiTF323390

Family and domain databases

Gene3Di1.20.58.1600, 1 hit
InterProiView protein in InterPro
IPR009332 Med22
IPR038427 MED22_sf
PANTHERiPTHR12434 PTHR12434, 1 hit
PfamiView protein in Pfam
PF06179 Med22, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A0JPN6-1) [UniParc]FASTAAdd to basket
Also known as: Surf5B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR
60 70 80 90 100
ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ
110 120 130 140 150
RNQQLRALQE ECDRKLITLR DEVSIDLYEL EEEYYSSSSS LCEANDLPLC
160 170 180 190 200
EAYWRLDLDT DSADGLSAPL LASPETGAGP LQSAAPVHSH GGGPGPTEHT
Length:200
Mass (Da):22,312
Last modified:December 12, 2006 - v1
Checksum:iBE3802B745AE5FC2
GO
Isoform 2 (identifier: A0JPN6-2) [UniParc]FASTAAdd to basket
Also known as: Surf5A

The sequence of this isoform differs from the canonical sequence as follows:
     138-140: SSS → RYK
     141-200: Missing.

Show »
Length:140
Mass (Da):16,436
Checksum:i8D79D73D81171AE1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028995138 – 140SSS → RYK in isoform 2. 1 Publication3
Alternative sequenceiVSP_028996141 – 200Missing in isoform 2. 1 PublicationAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC127519 mRNA Translation: AAI27520.1
DN932977 mRNA No translation available.
RefSeqiNP_001071147.1, NM_001077679.1 [A0JPN6-1]
XP_003749478.1, XM_003749430.3
UniGeneiRn.202721

Genome annotation databases

EnsembliENSRNOT00000006720; ENSRNOP00000006720; ENSRNOG00000005059 [A0JPN6-1]
ENSRNOT00000066193; ENSRNOP00000062301; ENSRNOG00000049749 [A0JPN6-1]
GeneIDi100911917
499762
KEGGirno:100911917
rno:499762
UCSCiRGD:1564893 rat [A0JPN6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED22_RAT
AccessioniPrimary (citable) accession number: A0JPN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 12, 2006
Last modified: July 18, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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