UniProtKB - A0JMQ9 (ZRN1B_DANRE)
Protein
Ubiquitin thioesterase zranb1-B
Gene
zranb1b
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration (By similarity).By similarity
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 451 | NucleophileBy similarity | 1 | |
Active sitei | 593 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 33 | RanBP2-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 82 – 111 | RanBP2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 152 – 181 | RanBP2-type 3PROSITE-ProRule annotationAdd BLAST | 30 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
- K63-linked polyubiquitin modification-dependent protein binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB
GO - Biological processi
- brain development Source: ZFIN
- cell migration Source: UniProtKB
- central nervous system morphogenesis Source: ZFIN
- cytoskeleton organization Source: UniProtKB
- positive regulation of Wnt signaling pathway Source: UniProtKB
- protein deubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
- protein K29-linked deubiquitination Source: UniProtKB
- protein K33-linked deubiquitination Source: UniProtKB
- protein K63-linked deubiquitination Source: UniProtKB
- regulation of cell morphogenesis Source: UniProtKB
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DRE-5689896, Ovarian tumor domain proteases |
Protein family/group databases
MEROPSi | C64.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin thioesterase zranb1-B (EC:3.4.19.12)Alternative name(s): Zinc finger Ran-binding domain-containing protein 1-B |
Gene namesi | Name:zranb1b Synonyms:zranb1 ORF Names:zgc:154177 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-061110-94, zranb1b |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000361555 | 1 – 716 | Ubiquitin thioesterase zranb1-BAdd BLAST | 716 |
Proteomic databases
PaxDbi | A0JMQ9 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000063190, Expressed in muscle organ and 31 other tissues |
Interactioni
GO - Molecular functioni
- K63-linked polyubiquitin modification-dependent protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 607517, 3 interactors |
STRINGi | 7955.ENSDARP00000086546 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 268 – 298 | ANK 1Add BLAST | 31 | |
Repeati | 321 – 348 | ANK 2Add BLAST | 28 | |
Domaini | 440 – 600 | OTUPROSITE-ProRule annotationAdd BLAST | 161 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 214 – 219 | Poly-Gly | 6 | |
Compositional biasi | 708 – 716 | Poly-Glu | 9 |
Domaini
The OTU domain mediates the deubiquitinating activity.By similarity
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity
Sequence similaritiesi
Belongs to the peptidase C64 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 33 | RanBP2-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 82 – 111 | RanBP2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 152 – 181 | RanBP2-type 3PROSITE-ProRule annotationAdd BLAST | 30 |
Keywords - Domaini
ANK repeat, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG4345, Eukaryota |
GeneTreei | ENSGT00940000158045 |
HOGENOMi | CLU_013907_0_0_1 |
InParanoidi | A0JMQ9 |
OMAi | IRNRMRR |
OrthoDBi | 728724at2759 |
PhylomeDBi | A0JMQ9 |
TreeFami | TF323312 |
Family and domain databases
InterProi | View protein in InterPro IPR041294, AnkUBD IPR003323, OTU_dom IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF18418, AnkUBD, 1 hit PF02338, OTU, 1 hit PF00641, zf-RanBP, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 3 hits |
SUPFAMi | SSF90209, SSF90209, 2 hits |
PROSITEi | View protein in PROSITE PS50802, OTU, 1 hit PS01358, ZF_RANBP2_1, 3 hits PS50199, ZF_RANBP2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
A0JMQ9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDLGLKWSC EYCTYENWPS AIKCTMCRAQ RHNAPIITEE PFKSSSSLDP
60 70 80 90 100
SLCTTQGGST LLICPDSSAR PRVRIADELP ETSSKWSCHM CTYLNWPRAI
110 120 130 140 150
RCTQCLSQRQ QGSQQHSPLS PSETPQTSGS RPSPVTSDPC EEYNDRNRLN
160 170 180 190 200
MHAQRWPCSA CTYENWPKSL RCVVCDHPKP SGSPETPQQD SEAESATSPS
210 220 230 240 250
IVNEQERENV RTAGGGGGGS RGRLRKLSPP MCKGQAEVKI ELASGAVGSD
260 270 280 290 300
NEQEADFKKL KQIRNRMRRS DWLFLNACAG VVEGDLAAVE AYKSSGGDIA
310 320 330 340 350
RQLTADEVRI LNRPSAFDAG FTLVHLAIRF QRQDMLAVLL TEVSQQTAKC
360 370 380 390 400
IPALVCPELT EQIRREVAAA LHRRKGEFPC YFFTDLVTFT LPADIEDLPP
410 420 430 440 450
NVQEKLFDEV LDRDVQKELE EESPIINWSL ELGTRLDSRL YALWNRTAGD
460 470 480 490 500
CLLDSVLQAT WGIYDKDSVL RKSLNDSLHD CSHWFYTRWK EWESWYSQSF
510 520 530 540 550
GLHFSLREEQ WQEDWAFILS LASQPGASLE QTHVFVLAHI LRRPIIVYGV
560 570 580 590 600
KYYKSFRGET LGYTRFQGVY LPLLWEQSFC WKSPIALGYT RGHFSALVAM
610 620 630 640 650
ENDGYDNRGA GANLNTDDDV TVTFLPLVDS ERKLLHIHFL SAQEMGTEEQ
660 670 680 690 700
QERMLRQWMD CCVTEGGVLV AMQKSSRRRN HPLVTQMVEK WLDGYRQLAA
710
CPTLSDGEEE EEDEDE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8PX38 | A0A2R8PX38_DANRE | Ubiquitinyl hydrolase 1 | zranb1b | 704 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC125972 mRNA Translation: AAI25973.1 |
RefSeqi | NP_001071236.1, NM_001077768.1 XP_005158652.1, XM_005158595.3 |
Genome annotation databases
Ensembli | ENSDART00000092113; ENSDARP00000086546; ENSDARG00000063190 |
GeneIDi | 777720 |
KEGGi | dre:777720 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC125972 mRNA Translation: AAI25973.1 |
RefSeqi | NP_001071236.1, NM_001077768.1 XP_005158652.1, XM_005158595.3 |
3D structure databases
SMRi | A0JMQ9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 607517, 3 interactors |
STRINGi | 7955.ENSDARP00000086546 |
Protein family/group databases
MEROPSi | C64.004 |
Proteomic databases
PaxDbi | A0JMQ9 |
Genome annotation databases
Ensembli | ENSDART00000092113; ENSDARP00000086546; ENSDARG00000063190 |
GeneIDi | 777720 |
KEGGi | dre:777720 |
Organism-specific databases
CTDi | 777720 |
ZFINi | ZDB-GENE-061110-94, zranb1b |
Phylogenomic databases
eggNOGi | KOG4345, Eukaryota |
GeneTreei | ENSGT00940000158045 |
HOGENOMi | CLU_013907_0_0_1 |
InParanoidi | A0JMQ9 |
OMAi | IRNRMRR |
OrthoDBi | 728724at2759 |
PhylomeDBi | A0JMQ9 |
TreeFami | TF323312 |
Enzyme and pathway databases
Reactomei | R-DRE-5689896, Ovarian tumor domain proteases |
Miscellaneous databases
PROi | PR:A0JMQ9 |
Gene expression databases
Bgeei | ENSDARG00000063190, Expressed in muscle organ and 31 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR041294, AnkUBD IPR003323, OTU_dom IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF18418, AnkUBD, 1 hit PF02338, OTU, 1 hit PF00641, zf-RanBP, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 3 hits |
SUPFAMi | SSF90209, SSF90209, 2 hits |
PROSITEi | View protein in PROSITE PS50802, OTU, 1 hit PS01358, ZF_RANBP2_1, 3 hits PS50199, ZF_RANBP2_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZRN1B_DANRE | |
Accessioni | A0JMQ9Primary (citable) accession number: A0JMQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | December 12, 2006 | |
Last modified: | December 2, 2020 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families