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UniProtKB - A0A5Q0QP64 (AGR4_AGRAE)
Protein
Bifunctional terpene synthase Agr4
Gene
Agr4
Organism
Agrocybe aegerita (Black poplar mushroom) (Agaricus aegerita)
Status
Functioni
Terpene cyclase that catalyzes the cyclization of farnesyl diphosphate (FPP) to various sesquiterpenes, including beta-copaene, alpha-cubebene, cadina-16,4-diene, gamma-muurolene, delta-cadinene, epizonarene, epicubenol and cubenol (PubMed:32233445).
Agr4 is also able to use the monoterpene precursor geranyl diphosphate (GPP) as substrates to synthesize the monoterpene beta-myrcene (PubMed:32233445).
Delta-cadinene is the major product of Agr4 (PubMed:32233445).
1 PublicationCatalytic activityi
- This reaction proceeds in the forward1 Publication direction.
- EC:4.2.3.1261 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.2.3.1271 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.2.3.151 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Mg2+1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 84 | Plays a critical role in the stabilization of intermediate cationBy similarity | 1 | |
Metal bindingi | 87 | Magnesium 1By similarity | 1 | |
Metal bindingi | 87 | Magnesium 2By similarity | 1 | |
Binding sitei | 176 | SubstrateBy similarity | 1 | |
Metal bindingi | 222 | Magnesium 3By similarity | 1 | |
Metal bindingi | 226 | Magnesium 3By similarity | 1 | |
Metal bindingi | 230 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- myrcene synthase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Lyase |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Agr41 Publication |
Organismi | Agrocybe aegerita (Black poplar mushroom) (Agaricus aegerita) |
Taxonomic identifieri | 5400 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetidae › Agaricales › Strophariaceae › Agrocybe |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000451259 | 1 – 342 | Bifunctional terpene synthase Agr4Add BLAST | 342 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 324 – 325 | Substrate-bindingBy similarity | 2 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 87 – 91 | DDXXD motifBy similarity | 5 |
Domaini
The DDXXD motif is important for the catalytic activity, presumably through binding to Mg2+.1 Publication
Sequence similaritiesi
Belongs to the terpene synthase family.Curated
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034686, Terpene_cyclase-like_2 |
SFLDi | SFLDG01020, Terpene_Cyclase_Like_2, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
A0A5Q0QP64-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSALPSQFKL PDLLSTCPLK DGTNPAYKKA AAESRAWIGS YNMFADRKRA
60 70 80 90 100
FFIQGQNELL CSHVYCYAGY EQLRTTCDFV NLLFVVDEVS DEQSGEDARA
110 120 130 140 150
TGQVFVNAMK YADWHDGSKL AKLTKDFRVR FLRLAGPKNV ARFVALCESY
160 170 180 190 200
TACVGKEAEL RESGQVLGVK EFIPLRRQNS AVLLCFSLVE YILGIDLDDE
210 220 230 240 250
VYRDENFLNA YWAACDHVCW ANDVYSYDME QSKGLSNNNI VTVLMEENHT
260 270 280 290 300
SLQDTSDYIG EKCAEFVQIY LTSKKRLSPS LGPDAALFLE SIGSWMVGNL
310 320 330 340
AWSFETSRYF GSRHLEVKET GIVILRPREL PEDGSSSDSD EE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | MN146027 Genomic DNA Translation: QGA30880.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | MN146027 Genomic DNA Translation: QGA30880.1 |
3D structure databases
AlphaFoldDBi | A0A5Q0QP64 |
SMRi | A0A5Q0QP64 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034686, Terpene_cyclase-like_2 |
SFLDi | SFLDG01020, Terpene_Cyclase_Like_2, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AGR4_AGRAE | |
Accessioni | A0A5Q0QP64Primary (citable) accession number: A0A5Q0QP64 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 7, 2020 |
Last sequence update: | February 26, 2020 | |
Last modified: | May 25, 2022 | |
This is version 8 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |