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Entry version 5 (10 Feb 2021)
Sequence version 1 (22 Apr 2020)
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Protein

Methionine synthase

Gene

metH

Organism
Oceanihabitans sp. IOP_32
Status
Unreviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetH route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Methionine synthase (metH)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetH route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi426Cobalt (cobalamin axial ligand)UniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei471CobalaminUniRule annotation1
Binding sitei614S-adenosyl-L-methionineUniRule annotation1
Binding sitei800S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei804Cobalamin; via carbonyl oxygenUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferaseUniRule annotationImported, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesisUniRule annotation
LigandCobalaminUniRule annotation, Cobalt, Metal-bindingUniRule annotation, S-adenosyl-L-methionineUniRule annotationARBA annotation, ZincUniRule annotation

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00051;UER00081

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Methionine synthaseUniRule annotation (EC:2.1.1.13UniRule annotation)
Alternative name(s):
5-methyltetrahydrofolate--homocysteine methyltransferaseUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:metHImported
ORF Names:FEZ18_10030Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiOceanihabitans sp. IOP_32Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri2529032 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeOceanihabitansunclassified Oceanihabitans
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000325630 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini323 – 406B12-binding N-terminalInterPro annotationAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni501 – 502Cobalamin-bindingUniRule annotation2
Regioni855 – 856S-adenosyl-L-methionine bindingUniRule annotation2

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin.UniRule annotation

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the vitamin-B12 dependent methionine synthase family.ARBA annotation

Keywords - Domaini

RepeatARBA annotation

Family and domain databases

Conserved Domains Database

More...
CDDi
cd02069, methionine_synthase_B12_BD, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.1240.10, 1 hit
3.10.196.10, 1 hit
3.20.20.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003759, Cbl-bd_cap
IPR006158, Cobalamin-bd
IPR036724, Cobalamin-bd_sf
IPR011005, Dihydropteroate_synth-like
IPR033706, Met_synthase_B12-bd
IPR011822, MetH
IPR036594, Meth_synthase_dom
IPR000489, Pterin-binding_dom
IPR004223, VitB12-dep_Met_synth_activ_dom
IPR037010, VitB12-dep_Met_synth_activ_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02310, B12-binding, 1 hit
PF02607, B12-binding_2, 1 hit
PF02965, Met_synt_B12, 1 hit
PF00809, Pterin_bind, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000381, MetH, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01018, B12-binding_2, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47644, SSF47644, 1 hit
SSF51717, SSF51717, 1 hit
SSF52242, SSF52242, 1 hit
SSF56507, SSF56507, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02082, metH, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A0A5Q0JXB5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVKQTKYMR LSGLEPLVLN ENSNFINVGE RTNVAGSRKF LRLIKEENYD
60 70 80 90 100
EALDIARHQV DGGAQILDVN FDDGLIDGKE AMIRFLNLIA AEPDICKVPI
110 120 130 140 150
MIDSSKWEII EAGLQVVQGK CVVNSISLKE GEANFIWEAK QIKRYGAAVI
160 170 180 190 200
VMAFDEVGQA DTYERRIEIA KRSYDILVDK VGFPSEDIIF DLNIFPVATG
210 220 230 240 250
MEEHRRNAID FIEATRWVRE NLPNVSVSGG VSNVSFSFRG NNGVREAMHS
260 270 280 290 300
VFLYHAIQAG MNIGIVNPAL LEVYDDIPKD LLEHVEDVIL DRRDDATERL
310 320 330 340 350
LDFAETVKGS KKEAGADLSW RENPLQDRIT HALVKGIDSY IVEDVEQARQ
360 370 380 390 400
EANSPIDVIE GHLMTGMNVV GDLFGAGKMF LPQVVKSARV MKKAVGYLNP
410 420 430 440 450
FIEAAKTDKQ EPVGKILMAT VKGDVHDIGK NIVSVVLACN NYEIVDLGVM
460 470 480 490 500
VPPEKIIETA IRERVDAIGL SGLITPSLDE MVYLAKEMQR NNLELPLLIG
510 520 530 540 550
GATTSKAHTA VKIDTQYNNA VVHVNDASRA VTVVGDLLNK KSSQDYVAKL
560 570 580 590 600
KKDYDEFRTK FLKRGKEKSY ISIEEARKRK YKIDWNATEI VKPNTLGIQV
610 620 630 640 650
LEQLSLKELL PFIDWSPFFR SWDLHGKFPD ILTDKVVGEQ ATIMYEEAQR
660 670 680 690 700
MIKVIIAKQL FKPKAIFGLF EANSIHHDDI SVIRKGKEIA VFRTLRQQLK
710 720 730 740 750
KREGIPNHAL ADFIAPKDSG KTDYLGLFSV GIFGAQELAE SYKLKDDDYN
760 770 780 790 800
AIMAQAIADR FAEALAEYLH KQIRMKHWGY AANENLTNDD LIKESYKGIR
810 820 830 840 850
PAPGYPACPD HLEKETIWEL LDVEKRIGIS LTESLAMWPA AAVSGYYFAN
860 870 880 890
PEAKYFGLGK ITDDQVTDYA KRKGITKAKA RKWLHANIAE
Length:890
Mass (Da):99,925
Last modified:April 22, 2020 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCEF4253824F3B7EE
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CP040813 Genomic DNA Translation: QFZ55105.1

Genome annotation databases

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
oci:FEZ18_10030

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP040813 Genomic DNA Translation: QFZ55105.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Genome annotation databases

KEGGioci:FEZ18_10030

Enzyme and pathway databases

UniPathwayiUPA00051;UER00081

Family and domain databases

CDDicd02069, methionine_synthase_B12_BD, 1 hit
Gene3Di1.10.1240.10, 1 hit
3.10.196.10, 1 hit
3.20.20.20, 1 hit
InterProiView protein in InterPro
IPR003759, Cbl-bd_cap
IPR006158, Cobalamin-bd
IPR036724, Cobalamin-bd_sf
IPR011005, Dihydropteroate_synth-like
IPR033706, Met_synthase_B12-bd
IPR011822, MetH
IPR036594, Meth_synthase_dom
IPR000489, Pterin-binding_dom
IPR004223, VitB12-dep_Met_synth_activ_dom
IPR037010, VitB12-dep_Met_synth_activ_sf
PfamiView protein in Pfam
PF02310, B12-binding, 1 hit
PF02607, B12-binding_2, 1 hit
PF02965, Met_synt_B12, 1 hit
PF00809, Pterin_bind, 1 hit
PIRSFiPIRSF000381, MetH, 1 hit
SMARTiView protein in SMART
SM01018, B12-binding_2, 1 hit
SUPFAMiSSF47644, SSF47644, 1 hit
SSF51717, SSF51717, 1 hit
SSF52242, SSF52242, 1 hit
SSF56507, SSF56507, 1 hit
TIGRFAMsiTIGR02082, metH, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiA0A5Q0JXB5_9FLAO
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A5Q0JXB5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: April 22, 2020
Last sequence update: April 22, 2020
Last modified: February 10, 2021
This is version 5 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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