UniProtKB - A0A5B9 (TRBC2_HUMAN)
T cell receptor beta constant 2
TRBC2
Functioni
Constant region of T cell receptor (TR) beta chain (PubMed:24600447).
Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed:25493333).
Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed:23524462).
The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed:15040585).
4 PublicationsGO - Molecular functioni
- antigen binding Source: GO_Central
- immunoglobulin receptor binding Source: GO_Central
GO - Biological processi
- B cell receptor signaling pathway Source: GO_Central
- complement activation, classical pathway Source: GO_Central
- defense response to bacterium Source: GO_Central
- innate immune response Source: GO_Central
- phagocytosis, engulfment Source: GO_Central
- phagocytosis, recognition Source: GO_Central
- positive regulation of B cell activation Source: GO_Central
Keywordsi
Molecular function | Receptor |
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
PathwayCommonsi | A0A5B9 |
Protein family/group databases
IMGT/GENE-DB | TRBC2 |
Names & Taxonomyi
Protein namesi | Recommended name: T cell receptor beta constant 21 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12157, TRBC2 |
MIMi | 615445, gene |
neXtProti | NX_A0A5B9 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Extracellular region or secreted
- immunoglobulin complex, circulating Source: GO_Central
Plasma Membrane
- alpha-beta T cell receptor complex Source: ComplexPortal
- external side of plasma membrane Source: GO_Central
- plasma membrane Source: ComplexPortal
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 145 – 167 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 168 – 178 | CytoplasmicSequence analysisAdd BLAST | 11 |
Keywords - Cellular componenti
Cell membrane, Membrane, T cell receptorPathology & Biotechi
Miscellaneous databases
Pharosi | A0A5B9, Tdark |
Genetic variation databases
BioMutai | TRBC2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000393471 | ‹1 – 178 | T cell receptor beta constant 2Add BLAST | ›178 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 95 | PROSITE-ProRule annotationCombined sources25 Publications | ||
Glycosylationi | 69 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 130 | Interchain (with C-94 in TRAC)By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | A0A5B9 |
MassIVEi | A0A5B9 |
PeptideAtlasi | A0A5B9 |
PRIDEi | A0A5B9 |
ProteomicsDBi | 1 |
PTM databases
GlyGeni | A0A5B9, 1 site |
iPTMneti | A0A5B9 |
PhosphoSitePlusi | A0A5B9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000211772, Expressed in granulocyte and 118 other tissues |
Interactioni
Subunit structurei
Alpha-beta TR is a heterodimer composed of an alpha and beta chain; disulfide-linked. The alpha-beta TR is associated with the transmembrane signaling CD3 coreceptor proteins to form the TR-CD3 (TcR or TCR). The assembly of alpha-beta TR heterodimers with CD3 occurs in the endoplasmic reticulum where a single alpha-beta TR heterodimer associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer and one CD247 homodimer forming a stable octomeric structure. CD3D-CD3E and CD3G-CD3E heterodimers preferentially associate with TR alpha and TR beta chains, respectively. The association of the CD247 homodimer is the last step of TcR assembly in the endoplasmic reticulum and is required for transport to the cell surface.
1 PublicationGO - Molecular functioni
- immunoglobulin receptor binding Source: GO_Central
Protein-protein interaction databases
ComplexPortali | CPX-6482, Alpha-beta T cell receptor complex, TRBC2 variant |
Miscellaneous databases
RNActi | A0A5B9, protein |
Structurei
Secondary structure
3D structure databases
SMRi | A0A5B9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | A0A5B9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 117 | Ig-like C1-typePROSITE-ProRule annotationAdd BLAST | 110 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 144 | Connecting peptideBy similarityAdd BLAST | 15 |
Domaini
Keywords - Domaini
Immunoglobulin domain, Transmembrane, Transmembrane helixPhylogenomic databases
HOGENOMi | CLU_077975_0_1_1 |
InParanoidi | A0A5B9 |
OMAi | KFLYIML |
PhylomeDBi | A0A5B9 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003597, Ig_C1-set |
Pfami | View protein in Pfam PF07654, C1-set, 1 hit |
SMARTi | View protein in SMART SM00407, IGc1, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
DLKNVFPPKV AVFEPSEAEI SHTQKATLVC LATGFYPDHV ELSWWVNGKE
60 70 80 90 100
VHSGVSTDPQ PLKEQPALND SRYCLSSRLR VSATFWQNPR NHFRCQVQFY
110 120 130 140 150
GLSENDEWTQ DRAKPVTQIV SAEAWGRADC GFTSESYQQG VLSATILYEI
160 170
LLGKATLYAV LVSALVLMAM VKRKDSRG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JMB4 | A0A0G2JMB4_HUMAN | T cell receptor beta constant 2 | TRBC2 | 179 | Annotation score: | ||
A0A5H1ZRR3 | A0A5H1ZRR3_HUMAN | T cell receptor beta constant 2 | TRBC2 | 179 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Sequence conflicti | 9 | K → E in AAC80214 (PubMed:8650574).Curated | 1 | |
Sequence conflicti | 9 | K → E in AAA60662 (PubMed:3860845).Curated | 1 | |
Sequence conflicti | 9 | K → E in EAW51905 (Ref. 4) Curated | 1 |
Polymorphismi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12888 Genomic DNA Translation: AAA60662.1 L36092 Genomic DNA Translation: AAC80214.1 AC245427 Genomic DNA No translation available. CH471198 Genomic DNA Translation: EAW51905.1 |
Genome annotation databases
UCSCi | uc064itq.1, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12888 Genomic DNA Translation: AAA60662.1 L36092 Genomic DNA Translation: AAC80214.1 AC245427 Genomic DNA No translation available. CH471198 Genomic DNA Translation: EAW51905.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AO7 | X-ray | 2.60 | E | 1-129 | [»] | |
1BD2 | X-ray | 2.50 | E | 1-129 | [»] | |
2EYR | X-ray | 2.40 | B | 1-129 | [»] | |
2EYS | X-ray | 2.21 | B | 1-129 | [»] | |
2EYT | X-ray | 2.60 | B/D | 1-129 | [»] | |
3KXF | X-ray | 3.10 | E/H/O/P | 1-129 | [»] | |
3O6F | X-ray | 2.80 | D/H | 1-130 | [»] | |
3O8X | X-ray | 2.74 | D | 13-128 | [»] | |
3O9W | X-ray | 2.80 | D | 13-128 | [»] | |
3QUX | X-ray | 2.91 | D | 13-128 | [»] | |
3T0E | X-ray | 4.00 | D | 1-130 | [»] | |
3TA3 | X-ray | 2.70 | D | 13-128 | [»] | |
3TVM | X-ray | 2.80 | D/H | 13-128 | [»] | |
4C56 | X-ray | 2.90 | B/H | 1-129 | [»] | |
4MVB | X-ray | 3.09 | D | 1-129 | [»] | |
4MXQ | X-ray | 2.60 | D | 1-129 | [»] | |
4N0C | X-ray | 2.90 | D/H | 1-129 | [»] | |
4N5E | X-ray | 3.06 | D | 1-129 | [»] | |
4NHU | X-ray | 2.90 | B/D | 1-147 | [»] | |
4ONH | X-ray | 3.01 | B | 1-129 | [»] | |
4P4K | X-ray | 2.80 | D/H | 1-129 | [»] | |
4UDT | X-ray | 1.35 | B | 1-129 | [»] | |
4UDU | X-ray | 2.50 | B | 1-129 | [»] | |
4WW1 | X-ray | 1.38 | B | 1-129 | [»] | |
4WW2 | X-ray | 2.48 | B | 1-129 | [»] | |
4WWK | X-ray | 3.10 | B | 1-129 | [»] | |
4Y16 | X-ray | 2.60 | D | 13-128 | [»] | |
4Y2D | X-ray | 3.05 | D/H | 13-128 | [»] | |
4Y4F | X-ray | 3.19 | D/H | 13-128 | [»] | |
4Y4H | X-ray | 3.10 | D/H | 13-128 | [»] | |
4Y4K | X-ray | 2.90 | D | 13-128 | [»] | |
4ZAK | X-ray | 2.82 | D | 18-128 | [»] | |
5BRZ | X-ray | 2.62 | E | 1-129 | [»] | |
5BS0 | X-ray | 2.40 | E | 1-129 | [»] | |
5C07 | X-ray | 2.11 | E/J | 1-129 | [»] | |
5C08 | X-ray | 2.33 | E/J | 1-129 | [»] | |
5C09 | X-ray | 2.48 | E/J | 1-129 | [»] | |
5C0A | X-ray | 2.46 | E/J | 1-129 | [»] | |
5C0B | X-ray | 2.03 | E/J | 1-129 | [»] | |
5C0C | X-ray | 1.97 | E/J | 1-129 | [»] | |
5EU6 | X-ray | 2.02 | E | 1-129 | [»] | |
5FK9 | X-ray | 3.10 | B | 1-129 | [»] | |
5FKA | X-ray | 2.40 | B | 1-129 | [»] | |
5HHM | X-ray | 2.50 | E/J | 1-129 | [»] | |
5HHO | X-ray | 2.95 | E | 1-129 | [»] | |
5HYJ | X-ray | 3.06 | E/J | 1-129 | [»] | |
5KS9 | X-ray | 2.55 | F/H | 1-129 | [»] | |
5KSA | X-ray | 2.00 | D | 1-129 | [»] | |
5KSB | X-ray | 2.90 | F/H | 1-129 | [»] | |
6CPH | X-ray | 1.70 | E | 1-129 | [»] | |
6CQL | X-ray | 2.40 | E | 1-129 | [»] | |
6CQQ | X-ray | 2.80 | E/J | 1-129 | [»] | |
6CQR | X-ray | 3.04 | E/J | 1-129 | [»] | |
6MIV | Other | 2.05 | D | 21-128 | [»] | |
6MIY | X-ray | 2.75 | D/H | 21-128 | [»] | |
6MJ4 | X-ray | 2.00 | D | 21-128 | [»] | |
6MJ6 | X-ray | 2.45 | D | 21-128 | [»] | |
6MJA | X-ray | 2.35 | D | 21-128 | [»] | |
6MJI | X-ray | 2.30 | D | 21-128 | [»] | |
6MJJ | X-ray | 1.93 | D | 21-128 | [»] | |
6MJQ | X-ray | 3.00 | D/H | 21-128 | [»] | |
SMRi | A0A5B9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-6482, Alpha-beta T cell receptor complex, TRBC2 variant |
Protein family/group databases
IMGT/GENE-DB | TRBC2 |
PTM databases
GlyGeni | A0A5B9, 1 site |
iPTMneti | A0A5B9 |
PhosphoSitePlusi | A0A5B9 |
Genetic variation databases
BioMutai | TRBC2 |
Proteomic databases
jPOSTi | A0A5B9 |
MassIVEi | A0A5B9 |
PeptideAtlasi | A0A5B9 |
PRIDEi | A0A5B9 |
ProteomicsDBi | 1 |
Protocols and materials databases
ABCDi | A0A5B9, 10 sequenced antibodies |
Genome annotation databases
UCSCi | uc064itq.1, human |
Organism-specific databases
GeneCardsi | TRBC2 |
HGNCi | HGNC:12157, TRBC2 |
MIMi | 615445, gene |
neXtProti | NX_A0A5B9 |
GenAtlasi | Search... |
Phylogenomic databases
HOGENOMi | CLU_077975_0_1_1 |
InParanoidi | A0A5B9 |
OMAi | KFLYIML |
PhylomeDBi | A0A5B9 |
Enzyme and pathway databases
PathwayCommonsi | A0A5B9 |
Miscellaneous databases
ChiTaRSi | TRBC2, human |
EvolutionaryTracei | A0A5B9 |
Pharosi | A0A5B9, Tdark |
PROi | PR:A0A5B9 |
RNActi | A0A5B9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000211772, Expressed in granulocyte and 118 other tissues |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003597, Ig_C1-set |
Pfami | View protein in Pfam PF07654, C1-set, 1 hit |
SMARTi | View protein in SMART SM00407, IGc1, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRBC2_HUMAN | |
Accessioni | A0A5B9Primary (citable) accession number: A0A5B9 Secondary accession number(s): A0A075B6S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 20, 2010 |
Last sequence update: | July 18, 2018 | |
Last modified: | February 23, 2022 | |
This is version 103 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references