UniProtKB - A0A4Z3 (A3LT2_RAT)
Alpha-1,3-galactosyltransferase 2
A3galt2
Functioni
Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.
3 PublicationsCatalytic activityi
- a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + UDP-α-D-galactose = an α-D-galactosyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + H+ + UDP1 PublicationEC:2.4.1.871 PublicationThis reaction proceeds in the forward1 Publication direction.
- a β-D-Gal-(1→4)-β-D-Glc-(1↔1)-Cer(d18:1(4E)) + UDP-α-D-galactose = H+ + isogloboside iGb3Cer (d18:1(4E)) + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 198 | ManganeseBy similarity | 1 | |
Metal bindingi | 200 | ManganeseBy similarity | 1 | |
Active sitei | 290 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- alpha-1,3-galactosyltransferase activity Source: MGI
- glycosyltransferase activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- N-acetyllactosaminide 3-alpha-galactosyltransferase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- cellular response to manganese ion Source: RGD
- glycosphingolipid biosynthetic process Source: UniProtKB
- lipid glycosylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Manganese, Metal-binding |
Protein family/group databases
CAZyi | GT6, Glycosyltransferase Family 6 |
Chemistry databases
SwissLipidsi | SLP:000000794 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-1,3-galactosyltransferase 2By similarity (EC:2.4.1.871 Publication)Alternative name(s): Isoglobotriaosylceramide synthase Short name: iGb3 synthase1 Publication Short name: iGb3S1 Publication |
Gene namesi | Name:A3galt2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 727913, A3galt2 |
Subcellular locationi
Golgi apparatus
- Golgi stack membrane 2 Publications; Single-pass type II membrane protein 2 Publications
Note: Also found in numerous large vesicles throughout the cytoplasm of the soma.
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi cisterna membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- vesicle Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 31 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 32 – 339 | LumenalSequence analysisAdd BLAST | 308 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 187 | L → P: Significant loss of activity. 1 Publication | 1 | |
Mutagenesisi | 198 – 200 | DVD → AVA: Catalytically inactive. 1 Publication | 3 | |
Mutagenesisi | 252 | Y → N: Complete loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000314872 | 1 – 339 | Alpha-1,3-galactosyltransferase 2Add BLAST | 339 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 57 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 99 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | A0A4Z3 |
PTM databases
GlyGeni | A0A4Z3, 2 sites |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000005935, Expressed in jejunum and 20 other tissues |
Genevisiblei | A0A4Z3, RN |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 107 – 112 | Substrate bindingBy similarity | 6 | |
Regioni | 198 – 200 | Substrate bindingBy similarity | 3 | |
Regioni | 220 – 223 | Substrate bindingBy similarity | 4 | |
Regioni | 332 – 338 | Substrate bindingBy similarity | 7 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QW2H, Eukaryota |
GeneTreei | ENSGT00950000182858 |
HOGENOMi | CLU_062445_1_0_1 |
InParanoidi | A0A4Z3 |
OMAi | LLLYWFP |
OrthoDBi | 1204439at2759 |
PhylomeDBi | A0A4Z3 |
TreeFami | TF330991 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005076, Glyco_trans_6 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR10462, PTHR10462, 1 hit |
Pfami | View protein in Pfam PF03414, Glyco_transf_6, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MALEGLRAKK RLLWRLFLSA FGLLGLYHYW FKIFRLFEVF IPMGICPMAI
60 70 80 90 100
MPLLKDNFTG VLRHWARPEV LTCTSWGAPI IWDETFDPHV AEREARRQNL
110 120 130 140 150
TIGLTVFAVG RYLEKYLEHF LVSAEQYFMV GQNVVYYVFT DRPEAVPHVA
160 170 180 190 200
LGQGRLLRVK PVRREKRWQD VSMARMLTLH EALGGQLGRE ADYVFCLDVD
210 220 230 240 250
QYFSGNFGPE VLADLVAQLH AWHFRWPRWM LPYERDKRSA AALSLSEGDF
260 270 280 290 300
YYHAAVFGGS VAALLKLTAH CATGQQLDRE HGIEARWHDE SHLNKFFWLS
310 320 330
KPTKLLSPEF CWAEEIGWRP EIHHPRLIWA PKEYALVRT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF248543 mRNA Translation: AAF82757.1 BC061714 mRNA Translation: AAH61714.1 |
RefSeqi | NP_612533.1, NM_138524.2 |
Genome annotation databases
Ensembli | ENSRNOT00000007851; ENSRNOP00000007851; ENSRNOG00000005935 |
GeneIDi | 171553 |
KEGGi | rno:171553 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF248543 mRNA Translation: AAF82757.1 BC061714 mRNA Translation: AAH61714.1 |
RefSeqi | NP_612533.1, NM_138524.2 |
3D structure databases
SMRi | A0A4Z3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000007851 |
Chemistry databases
SwissLipidsi | SLP:000000794 |
Protein family/group databases
CAZyi | GT6, Glycosyltransferase Family 6 |
PTM databases
GlyGeni | A0A4Z3, 2 sites |
Proteomic databases
PaxDbi | A0A4Z3 |
Genome annotation databases
Ensembli | ENSRNOT00000007851; ENSRNOP00000007851; ENSRNOG00000005935 |
GeneIDi | 171553 |
KEGGi | rno:171553 |
Organism-specific databases
CTDi | 127550 |
RGDi | 727913, A3galt2 |
Phylogenomic databases
eggNOGi | ENOG502QW2H, Eukaryota |
GeneTreei | ENSGT00950000182858 |
HOGENOMi | CLU_062445_1_0_1 |
InParanoidi | A0A4Z3 |
OMAi | LLLYWFP |
OrthoDBi | 1204439at2759 |
PhylomeDBi | A0A4Z3 |
TreeFami | TF330991 |
Miscellaneous databases
PROi | PR:A0A4Z3 |
Gene expression databases
Bgeei | ENSRNOG00000005935, Expressed in jejunum and 20 other tissues |
Genevisiblei | A0A4Z3, RN |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005076, Glyco_trans_6 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR10462, PTHR10462, 1 hit |
Pfami | View protein in Pfam PF03414, Glyco_transf_6, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A3LT2_RAT | |
Accessioni | A0A4Z3Primary (citable) accession number: A0A4Z3 Secondary accession number(s): A2JQW1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | November 28, 2006 | |
Last modified: | September 29, 2021 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families