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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Streptomyces griseus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotationSAAS annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26UDP-GlcNAcUniRule annotation1
Binding sitei79UDP-GlcNAcUniRule annotation1
Metal bindingi110MagnesiumUniRule annotation1
Binding sitei147UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei163UDP-GlcNAcUniRule annotation1
Binding sitei178UDP-GlcNAcUniRule annotation1
Metal bindingi236MagnesiumUniRule annotation1
Binding sitei236UDP-GlcNAcUniRule annotation1
Binding sitei341UDP-GlcNAcUniRule annotation1
Binding sitei359UDP-GlcNAcUniRule annotation1
Active sitei371Proton acceptorUniRule annotation1
Binding sitei374UDP-GlcNAcUniRule annotation1
Binding sitei385UDP-GlcNAcUniRule annotation1
Binding sitei388Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei413Acetyl-CoAUniRule annotation1
Binding sitei431Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei448Acetyl-CoAUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00113; UER00532
UPA00113; UER00533
UPA00973

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Gene namesi
Name:glmUUniRule annotation
ORF Names:BF14_020455Imported
OrganismiStreptomyces griseusImported
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000032367 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotrimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 139NTP_transf_3InterPro annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 238PyrophosphorylaseUniRule annotationAdd BLAST238
Regioni12 – 15UDP-GlcNAc bindingUniRule annotation4
Regioni84 – 85UDP-GlcNAc bindingUniRule annotation2
Regioni239 – 259LinkerUniRule annotationAdd BLAST21
Regioni260 – 482N-acetyltransferaseUniRule annotationAdd BLAST223
Regioni394 – 395Acetyl-CoA bindingUniRule annotation2

Sequence similaritiesi

In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Family and domain databases

CDDicd03353 LbH_GlmU_C, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_01631 GlmU, 1 hit
InterProiView protein in InterPro
IPR005882 Bifunctional_GlmU
IPR038009 GlmU_C_LbH
IPR001451 Hexapep
IPR025877 MobA-like_NTP_Trfase
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00132 Hexapep, 2 hits
PF12804 NTP_transf_3, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01173 glmU, 1 hit

Sequencei

Sequence statusi: Complete.

A0A2P5JRZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATSPAAVV VLAAGEGTRM KSKTPKVLHE ISGRSLVGHV VAAARELDPQ
60 70 80 90 100
HLVVVVGHAS EQVTAHLNAG DAPVRTAFQA EQNGTGHAVR MGLEELGGAV
110 120 130 140 150
DGTVIVVCGD TPLLSGETLG ALAATHSADA NAVTVLTAEV PDSTGYGRIV
160 170 180 190 200
RDPATGAVTE IVEHKDATDE QRAIREINSG VFAFDGRLLG DALGKVRTDN
210 220 230 240 250
SQGEEYLTDV LSILREAGHR VGASVAGDHR EILGINNRLQ LAEARRLLNE
260 270 280 290 300
RLLERAMLAG VTIVDPASTL IDATVTYERD AIVHPGTQLL GSTHLAEDSE
310 320 330 340 350
VGPNSRIEDT LVGTGARVDN TVTLSAEIGP GATVGPYAYL RPGTRLGIKA
360 370 380 390 400
KAGTYVEMKN ATIGEGTKVP HLSYVGDATI GDHTNIGAAS VFVNYDGVAK
410 420 430 440 450
HHTTIGSHCR TGSDNMFVAP VTVGDGVYTA AGSVITKDVP AGSLAVARGQ
460 470 480
QRNIEGWVAR KRPGSAAAQA AQTSSQEPDG QS
Length:482
Mass (Da):49,925
Last modified:May 23, 2018 - v1
Checksum:i7A011590B09FC224
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JYBE02000001 Genomic DNA Translation: PPA41853.1
RefSeqiWP_030803295.1, NZ_JYBE02000001.1

Similar proteinsi

Entry informationi

Entry nameiA0A2P5JRZ2_STRGR
AccessioniPrimary (citable) accession number: A0A2P5JRZ2
Entry historyiIntegrated into UniProtKB/TrEMBL: May 23, 2018
Last sequence update: May 23, 2018
Last modified: July 18, 2018
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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