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Protein

Polyribonucleotide 5'-hydroxyl-kinase Clp1

Gene

CLP1

Organism
Gorilla gorilla gorilla (Western lowland gorilla)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.UniRule annotation

Catalytic activityi

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.UniRule annotation
ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ni2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPUniRule annotation1
Binding sitei73ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 140ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotation, Transferase
Biological processmRNA processingUniRule annotationSAAS annotation, tRNA processingUniRule annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, NickelUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide 5'-hydroxyl-kinase Clp1UniRule annotation (EC:2.7.1.78UniRule annotation)
Alternative name(s):
Polyadenylation factor Clp1UniRule annotation
Polynucleotide kinase Clp1UniRule annotation
Pre-mRNA cleavage complex II protein Clp1UniRule annotation
Gene namesi
Name:CLP1UniRule annotationImported
OrganismiGorilla gorilla gorilla (Western lowland gorilla)Imported
Taxonomic identifieri9595 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeGorilla
Proteomesi
  • UP000001519 Componenti: Chromosome 11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 118CLP1_NInterPro annotationAdd BLAST92
Domaini132 – 318CLP1_PInterPro annotationAdd BLAST187
Domaini324 – 434Clp1InterPro annotationAdd BLAST111

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili104 – 124Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the Clp1 family. Clp1 subfamily.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

GeneTreeiENSGT00390000000344

Family and domain databases

Gene3Di2.60.120.1030, 1 hit
3.30.70.2410, 1 hit
HAMAPiMF_03035 Clp1, 1 hit
InterProiView protein in InterPro
IPR028606 Clp1
IPR010655 Clp1_C
IPR038238 Clp1_C_sf
IPR032324 Clp1_N
IPR038239 Clp1_N_sf
IPR032319 CLP1_P
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF06807 Clp1, 1 hit
PF16573 CLP1_N, 1 hit
PF16575 CLP1_P, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits

Sequencei

Sequence statusi: Complete.

A0A2I2ZMJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLHSSYTE EMGEEANDDK KPTTKFELER ETELRFEVEA SQSVQLELLT
60 70 80 90 100
GMAEIFGTEL TRNKKFTFDA GAKVAVFTWH GCSVQLSGRT EVAYVSKDTP
110 120 130 140 150
MLLYLNTHTA LEQMRRQAEK EEERGPRVMV VGPTDVGKST VCRLLLNYAV
160 170 180 190 200
RLGRRPTYVE LDVGQGSVSI PGTMGALYIE RPADVEEGFS IQAPLVYHFG
210 220 230 240 250
STTPGTNIKL YNKITSRLAD VFNQRCEVNR RASVSGCVIN TCGWVKGSGY
260 270 280 290 300
QALVHAASAF EVDVVVVLDQ ERLYNELKRD LPHFVRTVLL PKSGGVVERS
310 320 330 340 350
KDFRRECRDE RIREYFYGFR GCFYPHAFNV KFSDVKIYKV GAPTIPDSCL
360 370 380 390 400
PLGMSQEDNQ LKLVPVTPGR DMVHHLLSVS TAEGTEENLS ETSVAGFIVV
410 420 430
TSVDLEHQVF TVLSPAPRPL PKNFLLIMDI RFMDLK
Length:436
Mass (Da):48,940
Last modified:February 28, 2018 - v1
Checksum:i51576E4951199285
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CABD030079249 Genomic DNA No translation available.

Genome annotation databases

EnsembliENSGGOT00000051956; ENSGGOP00000048315; ENSGGOG00000023006

Similar proteinsi

Entry informationi

Entry nameiA0A2I2ZMJ9_GORGO
AccessioniPrimary (citable) accession number: A0A2I2ZMJ9
Entry historyiIntegrated into UniProtKB/TrEMBL: February 28, 2018
Last sequence update: February 28, 2018
Last modified: April 25, 2018
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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