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Protein

Lipoyl synthase

Gene

lipA

Organism
bacterium BMS3Bbin09
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi35Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi41Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi56Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi60Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi63Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationImported
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotationImported
ORF Names:BMS3Bbin09_00495Imported
Organismibacterium BMS3Bbin09Imported
Taxonomic identifieri2005732 [NCBI]
Taxonomic lineageiBacteria

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 250Elp3InterPro annotationAdd BLAST205

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

A0A2H6IQ29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHPEWIKIK AAGTHQTRKV LRHHGVSTVC EEARCPNQGQ CFTESVATFM
60 70 80 90 100
ILGDKCTRNC AFCSVESSTP LSLDPDEPQK VADAAKELGL KFVVITSVAR
110 120 130 140 150
DDLADGGSSQ FARTILTVRN ANKLIKIEVL TPDFKGNTEA LKIVLDARPD
160 170 180 190 200
VFNHNIETVP RLYPVVRPMA DYLTSVNMLK TAKEISLDIR TKSGIMLGLG
210 220 230 240 250
EEEQEVISVM QDLKEAGCDF LTIGQYLQPR RTNIPVVEYI RPEVFEEYRV
260 270 280
KGLEIGFKAV ASSPLTRSSM DAGEMFDKAE LSGA
Length:284
Mass (Da):31,450
Last modified:February 28, 2018 - v1
Checksum:iD7C809D63F897A6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BDTS01000046 Genomic DNA Translation: GBE40609.1

Similar proteinsi

Entry informationi

Entry nameiA0A2H6IQ29_9BACT
AccessioniPrimary (citable) accession number: A0A2H6IQ29
Entry historyiIntegrated into UniProtKB/TrEMBL: February 28, 2018
Last sequence update: February 28, 2018
Last modified: July 18, 2018
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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