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Protein

Siroheme synthase

Gene

cobA

Organism
Neisseria meningitidis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.UniRule annotationSAAS annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotationSAAS annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotationSAAS annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei246Proton acceptorUniRule annotation1
Active sitei268Proton donorUniRule annotation1
Binding sitei304S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei381S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei410S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation, MethyltransferaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation, Transferase
Biological processCobalamin biosynthesisUniRule annotationSAAS annotation, Porphyrin biosynthesisUniRule annotationSAAS annotation
LigandNADUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00148; UER00222
UPA00262; UER00211
UPA00262; UER00222
UPA00262; UER00376

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Including the following 3 domains:
Precorrin-2 dehydrogenaseUniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelataseUniRule annotation (EC:4.99.1.4UniRule annotation)
Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylaseUniRule annotation
Alternative name(s):
SUMTUniRule annotation
Uroporphyrinogen III methylaseUniRule annotation
Short name:
UROMUniRule annotation
Gene namesi
Name:cobAImported
Synonyms:cysGUniRule annotation
ORF Names:CNQ34_11050Imported
OrganismiNeisseria meningitidisImported
Taxonomic identifieri487 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000217930 Componenti: Unassembled WGS sequence

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128PhosphoserineUniRule annotation1

Keywords - PTMi

PhosphoproteinUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 145Sirohm_synth_MInterPro annotationAdd BLAST27
Domaini150 – 207CysG_dimeriserInterPro annotationAdd BLAST58
Domaini217 – 425TP_methylaseInterPro annotationAdd BLAST209

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST203
Regioni214 – 483Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST270
Regioni299 – 301S-adenosyl-L-methionine bindingUniRule annotation3
Regioni329 – 330S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di1.10.8.210, 1 hit
3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01646 Siroheme_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR036291 NAD(P)-bd_dom_sf
IPR037115 Sirohaem_synt_dimer_dom_sf
IPR012409 Sirohaem_synth
IPR028281 Sirohaem_synthase_central
IPR019478 Sirohaem_synthase_dimer_dom
IPR006367 Sirohaem_synthase_N
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF10414 CysG_dimeriser, 1 hit
PF14824 Sirohm_synth_M, 1 hit
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036426 Sirohaem_synth, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
TIGR01470 cysG_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0A2A3LTB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYFPIFANL AGRPVLVVGG GAVAARKISL LLKAGAEVRV AAKHLNAELS
60 70 80 90 100
ALAAENKILW LAEEFRAEHI RTVFLIIAAS SDQALNRRVF HLAESCQKPV
110 120 130 140 150
NVVDDRDHCS FIFPSVIDRN PVQIAVSSSG SAPVLARLLR ERLEALLPPS
160 170 180 190 200
LGDMAEISGR WRDAVKGKLK SVTERRRFWE KQFNGRFAAL VKNRQNTLAE
210 220 230 240 250
RELAKQLEQN YQGGFVSLVG AGPGDAGLLT LKGLQEIQQA DVVLYDALVS
260 270 280 290 300
DGILSLVRRD AERIFVGKRA RGGRTPQEDT NALMVRLARE GRRVVRLKGG
310 320 330 340 350
DPFVFGRGGE ELETLARHQI PFSVVPGITA AVGATAYAGI PLTHRDYAQS
360 370 380 390 400
AVFVTGHRKA DAPDIEWQTL ARSRQTLVIY MGALKAALIA ERLQQHGRSP
410 420 430 440 450
DTPAAVISQG TLPAQKTATG TLANLAELAE TAPNPALIVI GEVVGLHEKL
460 470 480
AWFGENAKKE SNPAEHAYFA LDGLGTGQEQ QAA
Length:483
Mass (Da):52,292
Last modified:December 20, 2017 - v1
Checksum:i80503BB2B6B0610E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
NTLY01000002 Genomic DNA Translation: PBJ88301.1
RefSeqiWP_002224535.1, NZ_NTLY01000002.1

Similar proteinsi

Entry informationi

Entry nameiA0A2A3LTB9_NEIME
AccessioniPrimary (citable) accession number: A0A2A3LTB9
Entry historyiIntegrated into UniProtKB/TrEMBL: December 20, 2017
Last sequence update: December 20, 2017
Last modified: June 20, 2018
This is version 7 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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