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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Salmonella enterica subsp. enterica serovar Heidelberg
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei563Aminoacyl-adenylateUniRule annotation1
Binding sitei607ATPUniRule annotation1
Metal bindingi907ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1
Metal bindingi927ZincUniRule annotation1
Metal bindingi930ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
ORF Names:CH228_22200Imported
OrganismiSalmonella enterica subsp. enterica serovar HeidelbergImported
Taxonomic identifieri611 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000216932 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 642tRNA-synt_1InterPro annotationAdd BLAST615
Domaini687 – 841Anticodon_1InterPro annotationAdd BLAST155
Domaini904 – 932zf-FPG_IleRSInterPro annotationAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionUniRule annotationAdd BLAST11
Motifi604 – 608"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationSAAS annotation

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0A265B0R6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGLSGFDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEQEF GKPGEKFTAA EFRAKCREYA ATQVDGQRKD FIRLGVLGDW
160 170 180 190 200
SHPYLTMDFK TEANIIRALG RIIKNGHLHK GAKPVHWCVD CRSALAEAEV
210 220 230 240 250
EYYDKTSPSI DVAFRAVDQD AVKAKFGLPG VSGPVSLVIW TTTPWTLPAN
260 270 280 290 300
RAISLAPDFD YALVQIDGQA VILAKDLVES VMQRIGAAEY TILGTVKGAE
310 320 330 340 350
LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDYVIGQKYG
360 370 380 390 400
LETANPVGPD GAYLPGTYPT LDGVNVFKAN DIVIELLKEK GALLHVEKMQ
410 420 430 440 450
HSYPCCWRHK TPIIFRATPQ WFVSMDKEGL RQQSLKEIKG VQWIPDWGQA
460 470 480 490 500
RIESMVANRP DWCISRQRTW GVPMSLFVHK ETQELLPIER TLAAMEEVAK
510 520 530 540 550
RVEVDGIQAW WDLDPKEILG EDADQYEKVP DTLDVWFDSG STSYSVVDAR
560 570 580 590 600
PEFAGHAADM YLEGSDQHRG WFMSSLMISV AMKGKAPYRQ VLTHGFTVDG
610 620 630 640 650
QGRKMSKSIG NTVSPQDVMN KLGADILRLW VASTDYTGEM AVSDEILKRA
660 670 680 690 700
ADSYRRIRNT ARFLLANLNG FNPATDMVKP EEMVVLDRWA VGCAKTAQQE
710 720 730 740 750
ILKAYEAYDF HEVVQRLMRF CSVEMGSFYL DIIKDRQYTA KADSVARRSC
760 770 780 790 800
QTALYHIAEA LVRWMAPIMS FTADEIWGYL PGEREKYVFT GEWYDGLFGL
810 820 830 840 850
EENEEFNDAF WDDVRYIKDQ VNKELENQKA NGIKSNLEAK VTLKYADDAN
860 870 880 890 900
GTIKKLKLLG EEVRFIFITS QFVISEQAGG IDDENIQYNA GNTTVQAVVT
910 920 930 940
RAEGDKCPRC WHYTTDVGKV AEHADICGRC VSNIAGNGEQ RKFA
Length:944
Mass (Da):105,642
Last modified:December 20, 2017 - v1
Checksum:iBF7EB1417D6D7744
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
NPEM01000028 Genomic DNA Translation: OZT38479.1
RefSeqiWP_001674865.1, NZ_PGWO01000010.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
NPEM01000028 Genomic DNA Translation: OZT38479.1
RefSeqiWP_001674865.1, NZ_PGWO01000010.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A265B0R6_SALET
AccessioniPrimary (citable) accession number: A0A265B0R6
Entry historyiIntegrated into UniProtKB/TrEMBL: December 20, 2017
Last sequence update: December 20, 2017
Last modified: May 23, 2018
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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