Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrate reductase

Gene

napA

Organism
Sporomusa silvacetica DSM 10669
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi52Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi56Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi84Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei86Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei146Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei171Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei175Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei343Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei347Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei453Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei500Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei527Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei717Substrate; via amide nitrogenUniRule annotation1
Binding sitei727Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei744Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotationImported
Biological processElectron transportUniRule annotationSAAS annotation, Nitrate assimilationUniRule annotationSAAS annotation, Transport
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, MolybdenumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotationImported
ORF Names:SPSIL_34190Imported
OrganismiSporomusa silvacetica DSM 10669Imported
Taxonomic identifieri1123289 [NCBI]
Taxonomic lineageiBacteriaFirmicutesNegativicutesSelenomonadalesSporomusaceaeSporomusa
Proteomesi
  • UP000216752 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Secreted UniRule annotation
  • Note: Membrane-associated.UniRule annotation

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_501312511433 – 753Nitrate reductaseSequence analysisAdd BLAST721

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 984Fe-4S Mo/W bis-MGD-typeInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni258 – 260Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni476 – 477Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni641 – 650Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.SAAS annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

A0A259UGH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSRRDFLK AIAVSTALVS SGCSLDSGGR GAQGTTSQHD AEKWVKGVCR
60 70 80 90 100
YCGVGCGVLA GVSKGKIVAV KGDPENTVNK GRLCVKGILL PKIMDTKDRL
110 120 130 140 150
LHPMVKKDGQ FVKVSWDEAL DLIATKFKEA IAQFGPDSVG YYGSGQNIAE
160 170 180 190 200
EAYVANKLFK GAIGTNNIDG NPRTCMASAV AGYVSTFGED EPMGAYADIE
210 220 230 240 250
QADVFFIIGA NMAEAHPVLY SRLVDRKNSN PNVKIIIVDP RRTRTADIAD
260 270 280 290 300
VFLQFVPGTD LALLNSMAYV IIEENLVDKD FVDQHTNFMQ GEKKLSFDEF
310 320 330 340 350
KTFLADYAPD KVTGLIDLPA EQIREVARLF AAKGRNTMSM WTMGLNQRIR
360 370 380 390 400
GTWANNLVHN LHLLTGKICR PGNTPFSLTG QPSACGSIRE VGALSHLLPA
410 420 430 440 450
HRVVANPKHR EEIAKIWGID PAKMSPKPGY HTIELFRAAA SGALKVLWVV
460 470 480 490 500
CTNPGQSIPN LKPYREGMEK TFMIVSETYH PTRTSELADV ILPAALWMEK
510 520 530 540 550
EGIYGNGERR TQHIAKAVEA PGEAKPDVWA LIEVAKRLGF GDLFPYKTND
560 570 580 590 600
EIWEEYRKCT IGTKMELPPY DRLKKERGLT WPVPDPNGPD TYIRYAAPYD
610 620 630 640 650
PYVKEGIQFY GKPDGRAVIF ARPHANPQEV PDAEYPFYLS TGRVLEHWHT
660 670 680 690 700
STMTMNVPEL KRAMPEMYVE LNQTDAGKIG VNDGELVKIT SRRGECQLKA
710 720 730 740 750
KINGRGQPRP GMVYVPFHDT EMIRMINFVT IDAFDDVSKQ PEYKICAVKL

AKA
Length:753
Mass (Da):83,500
Last modified:December 20, 2017 - v1
Checksum:i2425CD1C9C3AC629
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LSLK01000081 Genomic DNA Translation: OZC17054.1

Similar proteinsi

Entry informationi

Entry nameiA0A259UGH5_9FIRM
AccessioniPrimary (citable) accession number: A0A259UGH5
Entry historyiIntegrated into UniProtKB/TrEMBL: December 20, 2017
Last sequence update: December 20, 2017
Last modified: June 20, 2018
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health