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Protein

Thymidylate synthase

Gene

thyA

Organism
Klebsiella pneumoniae subsp. pneumoniae
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei146PROSITE-ProRule annotation1
Active sitei146NucleophileUniRule annotation1
Binding sitei1695,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei177dUMPUniRule annotation1
Binding sitei2635,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 127dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi166 – 169dUMPUniRule annotation4
Nucleotide bindingi207 – 209dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:AN676_0312305Imported, C4Y59_16635Imported, C4Y79_27860Imported, C4Y99_02155Imported, C4Z00_27215Imported, C4Z09_08080Imported, C4Z14_10005Imported, C4Z36_05500Imported, C5Y00_28225Imported, C5Y06_09360Imported, CLQ72_07885Imported
OrganismiKlebsiella pneumoniae subsp. pneumoniaeImported
Taxonomic identifieri72407 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000239447 Componenti: Unassembled WGS sequence
  • UP000238076 Componenti: Unassembled WGS sequence
  • UP000239540 Componenti: Unassembled WGS sequence
  • UP000238431 Componenti: Unassembled WGS sequence
  • UP000238125 Componenti: Unassembled WGS sequence
  • UP000239695 Componenti: Unassembled WGS sequence
  • UP000050263 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 264Thymidylat_syntInterPro annotationAdd BLAST263

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

KOiK00560

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1Y0PVG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLMQK VLTEGTPKND RTGTGTISIF GHQMRFNLQE GFPLVTTKRC
60 70 80 90 100
HLRSIIHELL WFLQGDTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRS
110 120 130 140 150
WPAPDGRHID QISTVMNQLK NDPDSRRIIV SAWNVGELDK MALAPCHAFF
160 170 180 190 200
QFYVADGKLS CQLYQRSCDV FLGLPFNIAS YALLVHMVAQ QCDLQVGDFV
210 220 230 240 250
WTGGDTHLYS NHLEQTNLQL SREPRPLPKL VIKRKPASIF DYRFEDFEIE
260
GYDPHPGIKA PVAI
Length:264
Mass (Da):30,293
Last modified:August 30, 2017 - v1
Checksum:iE5C48AD218062DAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP023553 Genomic DNA Translation: ATN99442.1
CP029384 Genomic DNA Translation: AWL44530.1
PTFW01000132 Genomic DNA Translation: PPM17335.1
PTGR01000079 Genomic DNA Translation: PPM88213.1
PTHJ01000027 Genomic DNA Translation: PPN00977.1
PTHE01000006 Genomic DNA Translation: PPN09558.1
PTIG01000034 Genomic DNA Translation: PPN91532.1
PTIL01000037 Genomic DNA Translation: PPO54113.1
PTHD01000119 Genomic DNA Translation: PPP47779.1
PVDS01000124 Genomic DNA Translation: PQW49149.1
PVDP01000043 Genomic DNA Translation: PQW61415.1
RefSeqiWP_002915933.1, NZ_PWAI01000001.1

Genome annotation databases

KEGGikpg:KPNIH32_22485
kpv:KPNIH29_21605
kpw:KPNIH30_21625
kpy:KPNIH31_20890

Similar proteinsi

Entry informationi

Entry nameiA0A1Y0PVG0_KLEPN
AccessioniPrimary (citable) accession number: A0A1Y0PVG0
Entry historyiIntegrated into UniProtKB/TrEMBL: August 30, 2017
Last sequence update: August 30, 2017
Last modified: July 18, 2018
This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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