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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycolicibacter algericus DSM 45454
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATP; via amide nitrogenUniRule annotation1
Metal bindingi125Magnesium; catalyticUniRule annotation1
Sitei126Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei149Proton acceptorUniRule annotation1
Binding sitei184Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei244SubstrateUniRule annotation1
Binding sitei286Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi83 – 84ATPUniRule annotation2
Nucleotide bindingi124 – 127ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processGlycolysisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
ORF Names:BST10_06670Imported
OrganismiMycolicibacter algericus DSM 45454Imported
Taxonomic identifieri723879 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacter
Proteomesi
  • UP000192693 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 318PFKInterPro annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 149Substrate bindingUniRule annotation3
Regioni191 – 193Substrate bindingUniRule annotation3
Regioni292 – 295Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1W9YI62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSGIKRVAI NTGGGDAPGL NAVIYAATLA AGNRGWEVVG IRDGFNGMLF
60 70 80 90 100
GDDYPDGGLI ELTRNRVRGI IHQGGTILGS TNRGNPIAYP VQQPDGSWIE
110 120 130 140 150
VDRTDELLGR FAERGIDALI TVGGDGSLTI GNQLHNAGLP LVGVPKTIDN
160 170 180 190 200
DLDRTASTFG FDSAVGFASE CIDRLFSTAT SHSRIIVVEV MGRYAGWIAL
210 220 230 240 250
HAGMASGVHA ILIPEIPYRL EPVAELIRER AQRGSTYSIV CVAEGAKPVG
260 270 280 290 300
GEHTVAAKAV GQAERLGGIG HKVATELEAM TGRESRAVVL GHLLRGGSPT
310 320 330 340 350
ALDRLLGLRF GAAAVRALDE GRRGVMVALN PPTVDYVPLA EATSRQKTVP
360 370
PDCDAIQTAR DMGIRFGDES LADG
Length:374
Mass (Da):39,279
Last modified:July 5, 2017 - v1
Checksum:i4CD664FB41B128DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MVHC01000005 Genomic DNA Translation: OQZ97870.1

Similar proteinsi

Entry informationi

Entry nameiA0A1W9YI62_9MYCO
AccessioniPrimary (citable) accession number: A0A1W9YI62
Entry historyiIntegrated into UniProtKB/TrEMBL: July 5, 2017
Last sequence update: July 5, 2017
Last modified: June 20, 2018
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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