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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Syntrophorhabdaceae bacterium PtaU1.Bin034
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73SubstrateUniRule annotation1
Metal bindingi210ManganeseUniRule annotation1
Metal bindingi230Manganese; via tele nitrogenUniRule annotation1
Binding sitei252SubstrateUniRule annotation1
Active sitei254UniRule annotation1
Metal bindingi277ManganeseUniRule annotation1
Binding sitei374GTPUniRule annotation1
Binding sitei405GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi253 – 258GTPUniRule annotation6
Nucleotide bindingi498 – 501GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase
Biological processGluconeogenesisUniRule annotation
LigandGTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

UniPathwayi
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinaseUniRule annotation
Short name:
GTP-PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotationImported
ORF Names:A4E57_01466Imported
OrganismiSyntrophorhabdaceae bacterium PtaU1.Bin034Imported
Taxonomic identifieri1811715 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophorhabdaceae

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 221PEPCK_NInterPro annotationAdd BLAST215
Domaini226 – 588PEPCK_CInterPro annotationAdd BLAST363

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni201 – 203Substrate bindingUniRule annotation3
Regioni372 – 374Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1V5CDX4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADIVEEWVQ EQAKLTKPDK IYWVRGTEEE MKALVNVGIK EEKIGIHNTF
60 70 80 90 100
QPLNPAAFPN SYLHRSHPTD VARTEHLTYV CLPKKEDTGP NNNWMHPEEA
110 120 130 140 150
LKLVNPLFDG CMSGRTLYVI PYMMGHPDSP YARPCVQITD SVYVVVSMYI
160 170 180 190 200
MTRVSARVLD IINSRGHFVK GLHSIGELDP EKRYIMHFPQ DDLVMSIGSG
210 220 230 240 250
YGGNALLGKK CISLRIASYL GYQQGWLAEH MIVMGVQDPE GDITYLLGSF
260 270 280 290 300
PSACGKTNLA MLDPIMEGYK VWTLGDDIAW INRGPDGRLY AINPEAGFFG
310 320 330 340 350
VAPGTSDKTN PYMMKTLRNN KFYPTLFTNT GLDEKTNSPW WEGLTDDIPE
360 370 380 390 400
PMLDWQGRPY DKKSGANVAH PNSRFTVSIY NCPTLSPEVD NPKGVPISGI
410 420 430 440 450
IFGGRRSNTI PLVCEAFDWE HGVFRASANG SETTAAATGQ AGLVRRDPMA
460 470 480 490 500
MLPFCGYNMA DYFGHWLRMG AESTQNPKIF FVNWFRKDGE GKFIWPGFRD
510 520 530 540 550
NSRVIKWMVD RIKGKVDARQ TPVGLMPHLN DLDLQGLSLP GSTIDKLFEV
560 570 580 590
DINEWRKEAA EIETFYARFG DRLPKALSKR LEELKANLGQ
Length:590
Mass (Da):66,365
Last modified:June 7, 2017 - v1
Checksum:i70D084A2F6250522
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MVRP01000027 Genomic DNA Translation: OPY68943.1

Similar proteinsi

Entry informationi

Entry nameiA0A1V5CDX4_9DELT
AccessioniPrimary (citable) accession number: A0A1V5CDX4
Entry historyiIntegrated into UniProtKB/TrEMBL: June 7, 2017
Last sequence update: June 7, 2017
Last modified: February 28, 2018
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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