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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Bacillus intestinalis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferaseUniRule annotation, Transferase
Biological processTranscription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
ORF Names:B2I22_12845Imported
OrganismiBacillus intestinalisImported
Taxonomic identifieri1963032 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000189784 Componenti: Unassembled WGS sequence

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymeraseUniRule annotationImported

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

SMRiA0A1V4FUC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 227RPOLDInterPro annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 230Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST230
Regioni247 – 314Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST68

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili231 – 251Sequence analysisAdd BLAST21

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
HAMAPiMF_00059 RNApol_bact_RpoA, 1 hit
InterProiView protein in InterPro
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR011773 DNA-dir_RpoA
IPR036603 RBP11-like
IPR011260 RNAP_asu_C
IPR036643 RNApol_insert_sf
PANTHERiPTHR32108 PTHR32108, 1 hit
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF03118 RNA_pol_A_CTD, 1 hit
PF01193 RNA_pol_L, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179 RNAP_asu_C, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
TIGRFAMsiTIGR02027 rpoA, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1V4FUC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIEKPKIE TVEISDDAKF GKFVVEPLER GYGTTLGNSL RRILLSSLPG
60 70 80 90 100
AAVTSIQIDG VLHEFSTIEG VVEDVTTIIL HIKKLALKIY SDEEKTLEID
110 120 130 140 150
VQGEGTVTAA DITHDSDVEI LNPDLHIATL GENASFRVRL TAQRGRGYTP
160 170 180 190 200
ADANKRDDQP IGVIPIDSIY TPVSRVSYQV ENTRVGQVAN YDKLTLDVWT
210 220 230 240 250
DGSTGPKEAI ALGSKILTEH LNIFVGLTDE AQHAEIMVEK EEDQKEKVLE
260 270 280 290 300
MTIEELDLSV RSYNCLKRAG INTVQELANK TEEDMMKVRN LGRKSLEEVK
310
AKLEELGLGL RKDD
Length:314
Mass (Da):34,799
Last modified:June 7, 2017 - v1
Checksum:i9ADCAD891C0BCD67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MWZB01000077 Genomic DNA Translation: OPG91220.1
RefSeqiWP_003225835.1, NZ_MWZB01000077.1

Similar proteinsi

Entry informationi

Entry nameiA0A1V4FUC0_9BACI
AccessioniPrimary (citable) accession number: A0A1V4FUC0
Entry historyiIntegrated into UniProtKB/TrEMBL: June 7, 2017
Last sequence update: June 7, 2017
Last modified: April 25, 2018
This is version 8 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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