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Protein

Kynurenine formamidase

Gene

AFMID

Organism
Alligator sinensis (Chinese alligator)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation

Catalytic activityi

N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 2,3-dioxygenase (TDO2)
  2. Kynurenine formamidase (AFMID)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90NucleophileUniRule annotation1
Active sitei173UniRule annotation1
Active sitei205UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation
Biological processTryptophan catabolismUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
Short name:
KFAUniRule annotation
Short name:
KFaseUniRule annotation
Alternative name(s):
ArylformamidaseUniRule annotation
N-formylkynurenine formamidaseUniRule annotation
Short name:
FKFUniRule annotation
Gene namesi
Name:AFMIDUniRule annotationImported
OrganismiAlligator sinensis (Chinese alligator)Imported
Taxonomic identifieri38654 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaCrocodyliaAlligatoridaeAlligatorinaeAlligator
Proteomesi
  • UP000189705 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 205Abhydrolase_3InterPro annotationAdd BLAST189

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 25HGGXWUniRule annotation5

Domaini

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

Sequence similaritiesi

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

KOiK01432

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1U7SFU6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
ALDVYLPAAP AGAFPLVLYV HGGYWQFLSK EESGFAAPPL LSRGIALVAV
60 70 80 90 100
GYDVAPRGNM DVMVAQVRRS VACIVRQYPT TSGVYLCGHS AGAHLAAMVL
110 120 130 140 150
ATDWQEYRVA PDIKGAFLVS GIYDLEPIVS TYVNDALRMT PEVAQRNSPL
160 170 180 190 200
LHVAAATPAT EACEVLVAVA QHDSPEFHRQ SRQYFQALRS AGWKVSLLDV
210 220 230
AHTDHFDVVE KLSQEGYILT QVILSAIARA
Length:230
Mass (Da):24,908
Last modified:May 10, 2017 - v1
Checksum:iC0DE0CB1E1B95B4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1Imported1

Sequence databases

RefSeqiXP_006036978.2, XM_006036916.2

Genome annotation databases

KEGGiasn:102382271

Similar proteinsi

Cross-referencesi

Sequence databases

RefSeqiXP_006036978.2, XM_006036916.2

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiasn:102382271

Organism-specific databases

CTDi125061

Phylogenomic databases

KOiK01432

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A1U7SFU6_ALLSI
AccessioniPrimary (citable) accession number: A0A1U7SFU6
Entry historyiIntegrated into UniProtKB/TrEMBL: May 10, 2017
Last sequence update: May 10, 2017
Last modified: September 12, 2018
This is version 9 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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