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Protein

Pup--protein ligase

Gene

pafA_1

Organism
Mycobacteroides abscessus subsp. abscessus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.UniRule annotation

Miscellaneous

The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.UniRule annotation

Catalytic activityi

ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine.UniRule annotationSAAS annotation

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

Pathwayi: protein pupylation

This protein is involved in the pathway protein pupylation, which is part of Protein modification.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway protein pupylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37MagnesiumUniRule annotation1
Binding sitei81ATPUniRule annotation1
Metal bindingi83MagnesiumUniRule annotation1
Active sitei85Proton acceptorUniRule annotation1
Metal bindingi91MagnesiumUniRule annotation1
Binding sitei94ATP; via carbonyl oxygenUniRule annotation1
Binding sitei447ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processUbl conjugation pathwayUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00997
UPA00998

Names & Taxonomyi

Protein namesi
Recommended name:
Pup--protein ligaseUniRule annotation (EC:6.3.1.19UniRule annotation)
Alternative name(s):
Proteasome accessory factor AUniRule annotation
Pup-conjugating enzymeUniRule annotation
Gene namesi
Name:pafA_1Imported
Synonyms:pafAUniRule annotation
ORF Names:SAMEA2259654_02059Imported
OrganismiMycobacteroides abscessus subsp. abscessusImported
Taxonomic identifieri1185650 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteroides abscessus

Family & Domainsi

Sequence similaritiesi

Belongs to the Pup ligase/Pup deamidase family. Pup-conjugating enzyme subfamily.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_02111 Pup_ligase, 1 hit
InterProiView protein in InterPro
IPR022279 Pup_ligase
IPR004347 Pup_ligase/deamidase
PANTHERiPTHR42307 PTHR42307, 1 hit
PfamiView protein in Pfam
PF03136 Pup_ligase, 1 hit
PIRSFiPIRSF018077 UCP018077, 1 hit
TIGRFAMsiTIGR03686 pupylate_PafA, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1U0P7E6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRYPDRSA RERPASPLSV ASTSRLDDVQ RRIMGIETEF GVTCTFHGHR
60 70 80 90 100
RLSPDEVARY LFRRVVSWGR SSNVFLRNGA RLYLDVGSHP EYATAECDSL
110 120 130 140 150
VQLVTHDRAG ERVLEDLLVD AEQRLSDEGI GGDIYLFKNN TDSAGNSYGC
160 170 180 190 200
HENYLIVRAG EFSRISDVLL PFLVTRQLIC GAGKVLQTPK AATYCLSQRA
210 220 230 240 250
EHIWEGVSSA TTRSRPIINT RDEPHADAEK YRRLHVIVGD SNMAETTTML
260 270 280 290 300
KVGTAALVLE MIESGVAFRD FSLDNPIRAI REVSHDLTGR RPVRLAGGRQ
310 320 330 340 350
ASALDIQREY HARAVEHLNT REPNEQVEQV VELWGRTLDA VESQDFSKVD
360 370 380 390 400
TEIDWVIKRK LFQRYQDRYN MELSDPKIAQ LDLAYHDIKR GRGVFDLLQR
410 420 430 440 450
KGLAARVTTD EEIKAAVDQP PQTTRAKLRG DFITAAQEAG RDFTVDWVHL
460 470 480
KLNDQAQRTV LCKDPFRSVD ERVERLIASM
Length:480
Mass (Da):54,406
Last modified:May 10, 2017 - v1
Checksum:i26B8329007A4227E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FVLU01000093 Genomic DNA Translation: SKS39938.1

Similar proteinsi

Entry informationi

Entry nameiA0A1U0P7E6_9MYCO
AccessioniPrimary (citable) accession number: A0A1U0P7E6
Entry historyiIntegrated into UniProtKB/TrEMBL: May 10, 2017
Last sequence update: May 10, 2017
Last modified: June 20, 2018
This is version 6 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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