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Entry version 17 (02 Jun 2021)
Sequence version 1 (10 May 2017)
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Protein

(-)-kolavenyl diphosphate synthase, chloroplastic

Gene

KPS

Organism
Salvia divinorum (Maria pastora) (Diviner's sage)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the biosynthesis of clerodane diterpenoids natural products, including salvinorin A with potent agonistic activity on brain kappa-opioid receptors, thus confering hallucinogenic properties (PubMed:30468448).

Diterpene synthase that catalyzes the formation of (-)-kolavenyl diphosphate from geranylgeranyl diphosphate (GGPP) as the first reaction in salvinorin A biosynthesis (PubMed:27865008, PubMed:28204567).

1 Publication2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by high concentrations of magnesium.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.88 with geranylgeranyl diphosphate as substrate.1 Publication
  1. KM=1.9 µM for geranylgeranyl diphosphate1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei237SubstrateBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi368MagnesiumBy similarity1
    Metal bindingi370MagnesiumBy similarity1
    Binding sitei454SubstrateBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionIsomerase
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    5.5.1.28, 15072
    5.5.1.B7, 15072

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    (-)-kolavenyl diphosphate synthase, chloroplastic1 Publication (EC:5.5.1.282 Publications)
    Short name:
    SdKPS1 Publication
    Alternative name(s):
    Clerodienyl diphosphate synthase1 Publication
    Kolavenyl diphosphate synthase CPS21 Publication
    Short name:
    SdCPS21 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:KPS1 Publication
    Synonyms:CPS21 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSalvia divinorum (Maria pastora) (Diviner's sage)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri28513 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeSalviaSalvia subgen. Calosphace

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Chloroplast, Plastid

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi200V → I: No switch of product outcome. 1 Publication1
    Mutagenesisi255F → H: Complete switch of product outcome from (-)-kolavenyl diphosphate to ent-copalyl diphosphate. 2 Publications1
    Mutagenesisi314A → V: No switch of product outcome. 1 Publication1
    Mutagenesisi359C → F: Catalyzes the production of ent-7,13E-copalyl diphosophate; when associated with S-360. 1 Publication1
    Mutagenesisi360W → A: Catalyzes the production of ent-8alpha/beta-labda-13-en-8-ol diphosphate. 1 Publication1
    Mutagenesisi360W → S: Catalyzes the production of ent-8alpha/beta-labda-13-en-8-ol diphosphate and of ent-7,13E-copalyl diphosophate. Increased ability to produce ent-7,13E-copalyl diphosophate; when associated with F-359. 1 Publication1
    Mutagenesisi369S → V: No switch of product outcome. 1 Publication1
    Mutagenesisi402S → C: No switch of product outcome. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 47ChloroplastSequence analysisAdd BLAST47
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000044768848 – 787(-)-kolavenyl diphosphate synthase, chloroplasticAdd BLAST740

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Expressed in peltate glandular trichomes of leaves (PubMed:27865008). Highly expressed in the first leaf pair (PubMed:28204567).2 Publications

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    A0A1S5RW73

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi368 – 371DXDD motif1 Publication4

    <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity through binding to Mg2+.1 Publication

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the terpene synthase family. Tpsc subfamily.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.600.10, 1 hit
    1.50.10.130, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR008949, Isoprenoid_synthase_dom_sf
    IPR001906, Terpene_synth_N
    IPR036965, Terpene_synth_N_sf
    IPR005630, Terpene_synthase_metal-bd
    IPR008930, Terpenoid_cyclase/PrenylTrfase

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF01397, Terpene_synth, 1 hit
    PF03936, Terpene_synth_C, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF48239, SSF48239, 2 hits
    SSF48576, SSF48576, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    A0A1S5RW73-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSFATSLPRP TTTGAAGFGL PLATCISLSV SHSFSPKFGI CNNTSLRLKS
    60 70 80 90 100
    KAGSGCYEGI HRSQLAASTI LEGHTPINPE VESEKIRLIE RIRLMFRSMD
    110 120 130 140 150
    DGEISVSPYD TAWVALVEDI GGSGGPQFPT SLEWISNNQL DDGSWGDRKF
    160 170 180 190 200
    VLYDRILNTL ACVVALTTWK MHPNKCEKGL RFISDNIEKL ADEDEELMPV
    210 220 230 240 250
    GFEIALPSLI DLAKRLCIEI PDNSASIKNI YAKRDSKLKR IPMDLMHKKP
    260 270 280 290 300
    TSLLFSLEGM EGLNWDKLLD FQSEGSFLSS PSSTAYALHH TKDELCLEYL
    310 320 330 340 350
    LKAVKKFNGG VPNAYPVDMF EHLWSVDRLR RLGISRYFQV EIDECLDYVY
    360 370 380 390 400
    RYWTNKGICW ARNMCVQDSD DSSMGFRLLR LYGYDVSIDV FKQFEEGGQF
    410 420 430 440 450
    CSIPGQMTHA ITGMYNLYRA SQLMFPQEHI LADARNFTAN LLHQKRVTNS
    460 470 480 490 500
    IVDKWIITKD LPGEVAYALD VPFYASLPRL EARFFLEQYG GDDDVWIGKT
    510 520 530 540 550
    LYRMLYVNCN TYLELAKLDY KHCQTVHQLE WNSMQTWYRE CNLGEFGLSE
    560 570 580 590 600
    RSLLLAYYIA ASTAFEPEKS SERLAWAITT ILVETIMSQE LSDEQKREFV
    610 620 630 640 650
    DEFVNISIIN NQNGGRYKPG NRLVEVLINT VTLMAEGRGT DQQLSNAWKN
    660 670 680 690 700
    WLKTWEEGGD LGEAEARLLL HTIHLSSGLD ESSFSHPKYQ QLLEATSKVC
    710 720 730 740 750
    HQLRLFQNLK ANDAQGSTSR LVTVTTFQIE AGMQELVKLI FTKTLEDLTS
    760 770 780
    ATKQSFFNIA RSFYYTAYCP ADTIDSHINK VLFEKIV
    Length:787
    Mass (Da):89,377
    Last modified:May 10, 2017 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1015581F3C0DC1FA
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti308N → H in APH81400 (PubMed:27865008).Curated1
    Sequence conflicti643Q → L in APH81400 (PubMed:27865008).Curated1
    Sequence conflicti710K → KQ in APH81400 (PubMed:27865008).Curated1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    KX268505 mRNA Translation: AOZ15895.1
    KX424877 mRNA Translation: APH81400.1

    Genome annotation databases

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ag:AOZ15895

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    KX268505 mRNA Translation: AOZ15895.1
    KX424877 mRNA Translation: APH81400.1

    3D structure databases

    SMRiA0A1S5RW73
    ModBaseiSearch...

    Genome annotation databases

    KEGGiag:AOZ15895

    Enzyme and pathway databases

    BRENDAi5.5.1.28, 15072
    5.5.1.B7, 15072

    Family and domain databases

    Gene3Di1.10.600.10, 1 hit
    1.50.10.130, 1 hit
    InterProiView protein in InterPro
    IPR008949, Isoprenoid_synthase_dom_sf
    IPR001906, Terpene_synth_N
    IPR036965, Terpene_synth_N_sf
    IPR005630, Terpene_synthase_metal-bd
    IPR008930, Terpenoid_cyclase/PrenylTrfase
    PfamiView protein in Pfam
    PF01397, Terpene_synth, 1 hit
    PF03936, Terpene_synth_C, 1 hit
    SUPFAMiSSF48239, SSF48239, 2 hits
    SSF48576, SSF48576, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiKPS_SALDI
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A1S5RW73
    Secondary accession number(s): A0A1L3THX5
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 31, 2019
    Last sequence update: May 10, 2017
    Last modified: June 2, 2021
    This is version 17 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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