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Protein

Kynurenine formamidase

Gene

afmid

Organism
Salmo salar (Atlantic salmon)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation

Catalytic activityi

N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 2,3-dioxygenase (tdo2), Tryptophan 2,3-dioxygenase (LOC106611580), Tryptophan 2,3-dioxygenase (tdo2)
  2. Kynurenine formamidase (afmid), Kynurenine formamidase (afmid), Kynurenine formamidase (afmid), Kynurenine formamidase (afmid)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei153NucleophileUniRule annotation1
Active sitei236UniRule annotation1
Active sitei269UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation
Biological processTryptophan catabolismUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
Short name:
KFAUniRule annotation
Short name:
KFaseUniRule annotation
Alternative name(s):
ArylformamidaseUniRule annotation
N-formylkynurenine formamidaseUniRule annotation
Short name:
FKFUniRule annotation
Gene namesi
Name:afmidImported
Synonyms:AFMIDUniRule annotation, FKFImported, KFImported, KFAImported
OrganismiSalmo salar (Atlantic salmon)Imported
Taxonomic identifieri8030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo
Proteomesi
  • UP000087266 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

SMRiA0A1S2X277
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 269Abhydrolase_3InterPro annotationAdd BLAST190

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi84 – 88HGGXWUniRule annotation5

Domaini

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

Sequence similaritiesi

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

KOiK01432

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

A0A1S2X277-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRWKDMNKD ELERQFSPSQ WSHRMSADDV IKSHVTALKE GTERARGLAQ
60 70 80 90 100
TLLNVPYGEG EGEKLDVYVP TTTSLDVPLV IYLHGGYWQF LSKEESGFMA
110 120 130 140 150
VPLVHKGVVV VAVGYDIAPK GNMDVMVSQV RRSVVSVIQQ YSHISGLYLC
160 170 180 190 200
GHSAGAHLAA MILSTDWSQY SVTPQIKGAF LVSGIYDLLP ILSTYVNEPL
210 220 230 240 250
KMTEEVALRN SPSQLVPQLK LSSSNCDIVV AVAQNDSPEF RKQSEDYYKA
260 270 280 290
LESTEGLKVT LEDVPNTDHF NIIEQLVDGD YHLTQLLLKM MGKS
Length:294
Mass (Da):32,682
Last modified:April 12, 2017 - v1
Checksum:i8ABC38B02E85DF67
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1S3QH98A0A1S3QH98_SALSA
Kynurenine formamidase
afmid AFMID, FKF, KF, KFA
308Annotation score:
A0A1S3QG46A0A1S3QG46_SALSA
Kynurenine formamidase
afmid AFMID, FKF, KF, KFA
270Annotation score:
B5XB27KFA_SALSA
Kynurenine formamidase
afmid
294Annotation score:

Sequence databases

RefSeqiNP_001134570.1, NM_001141098.1
XP_014038735.1, XM_014183260.1
UniGeneiSsa.2978

Genome annotation databases

GeneIDi100196069
KEGGisasa:100196069

Similar proteinsi

Cross-referencesi

Sequence databases

RefSeqiNP_001134570.1, NM_001141098.1
XP_014038735.1, XM_014183260.1
UniGeneiSsa.2978

3D structure databases

SMRiA0A1S2X277
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100196069
KEGGisasa:100196069

Organism-specific databases

CTDi125061

Phylogenomic databases

KOiK01432

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A1S2X277_SALSA
AccessioniPrimary (citable) accession number: A0A1S2X277
Entry historyiIntegrated into UniProtKB/TrEMBL: April 12, 2017
Last sequence update: April 12, 2017
Last modified: February 28, 2018
This is version 8 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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