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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio palustris
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
Gene namesi
Name:glnDUniRule annotationImported
ORF Names:VPAL9027_00149Imported
OrganismiVibrio palustrisImported
Taxonomic identifieri1918946 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000189475 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini451 – 573HDInterPro annotationAdd BLAST123
Domaini693 – 777ACTInterPro annotationAdd BLAST85
Domaini800 – 874ACTInterPro annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 332UridylyltransferaseUniRule annotationAdd BLAST332

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatSAAS annotation

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 1 hit
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1R4AZZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFQAPSHLN NDELSIPDLK DQLDQFAQEQ KAQFLQHHSV SDLVHGRSHY
60 70 80 90 100
MDELLARLWR FYQFDTLTEL SLVAVGGYGR GELHPLSDID MLIVSQHPLS
110 120 130 140 150
EDVGVKISQF ITFLWDLRLE VGHAVRTVQE CADIGRADLT VATNLQEARL
160 170 180 190 200
LCGSHETFEQ LKDVIVSESF WPSNVFYKAK LQEQRARHAR YHDTTYNLEP
210 220 230 240 250
DIKSTPGGLR DIHTLSWIAR RHFGATSLYE MSRYDFLTDA EYRELVECQE
260 270 280 290 300
FLWRVRFALH LELRRYDNRL TFGHQANVAE HLGFTGDGNR AVEKMMKEFY
310 320 330 340 350
RTLRRVVELN RMLLMLFDQA ILNHETDEQC IVLDDDFARC GPWIQARHPA
360 370 380 390 400
LFQARPESIL DMFIHMADDT SITGVSPTTM RQLRTARRRL NTFLHSIPSA
410 420 430 440 450
RDKFMTLVRH PNSLQNAFPQ MHELGVMAAY LPQWSHIVGQ MQFDLFHVYT
460 470 480 490 500
VDEHTIRVLK QLQTFHVPEN YNTHPICCEI FPRYQKRELI ILAAIFHDIG
510 520 530 540 550
KGRNGDHSEI GAKESYDFCL EHGLSKPEAR LVSWLVQNHL LMSITAQRRD
560 570 580 590 600
IYDPDVISDF AKTVREEEAL DALVCLTVAD ICATNPELWN SWKRTLLAEL
610 620 630 640 650
YYSTQRALRR GLENPVDVRE RVRHNQQMAS AHLRKEGYTP QHIDRLWLRF
660 670 680 690 700
KADYFLRHSD KQIAWHCSNI LKLTENSPPL VLINKRPTRG ITEIFVYTKD
710 720 730 740 750
QPALFANVVA ELDRRNLNVH DAQVMTSKDG YVLDTFMVLD NNGDAIVESE
760 770 780 790 800
HEALIGHIHK AVAKGKQLHQ KTRRLPRQLK HFKVKTQVDF LPTKSKKRTL
810 820 830 840 850
IELVALDTPG LLATIGATFS ELNLDLHGAK ITTIGERAED LFILTRHGGG
860 870
RLSEQEAIEL REALIENIVE LSPN
Length:874
Mass (Da):100,981
Last modified:April 12, 2017 - v1
Checksum:i7C33927060BA3A2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FUFT01000001 Genomic DNA Translation: SJL82238.1

Similar proteinsi

Entry informationi

Entry nameiA0A1R4AZZ7_9VIBR
AccessioniPrimary (citable) accession number: A0A1R4AZZ7
Entry historyiIntegrated into UniProtKB/TrEMBL: April 12, 2017
Last sequence update: April 12, 2017
Last modified: July 18, 2018
This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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