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Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Pseudomonas moraviensis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotationSAAS annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei267ATPUniRule annotation1
Binding sitei289ATP; via amide nitrogenUniRule annotation1
Binding sitei298ATPUniRule annotation1
Active sitei457Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 186ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, TransferaseUniRule annotationSAAS annotation, tRNA-bindingUniRule annotationSAAS annotation
Biological processThiamine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA sulfurtransferaseUniRule annotationSAAS annotation (EC:2.8.1.4UniRule annotationSAAS annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
ORF Names:BSZ28_07245Imported
OrganismiPseudomonas moraviensisImported
Taxonomic identifieri321662 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000185572 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Keywords - PTMi

Disulfide bondSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 167THUMPInterPro annotationAdd BLAST105
Domaini405 – 483RhodaneseInterPro annotationAdd BLAST79

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotationSAAS annotation

Keywords - Domaini

Redox-active centerSAAS annotation

Family and domain databases

CDDicd01712 ThiI, 1 hit
Gene3Di3.40.250.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00021 ThiI, 1 hit
InterProiView protein in InterPro
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR014729 Rossmann-like_a/b/a_fold
IPR026340 Thiazole_biosynth_dom
IPR020536 ThiI_AANH
IPR004114 THUMP_dom
IPR003720 tRNA_STrfase
PfamiView protein in Pfam
PF02568 ThiI, 1 hit
PF02926 THUMP, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
SUPFAMiSSF52821 SSF52821, 1 hit
TIGRFAMsiTIGR04271 ThiI_C_thiazole, 1 hit
TIGR00342 TIGR00342, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS51165 THUMP, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1Q3I9R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIVKVFPE ITIKSRPVRT KFIRQLAKNI RTVLRDLDPA VVVNGVWDNL
60 70 80 90 100
ELETRVTDPK ALKEMGERLT CMPGIAHFLQ IDEYPLGDFD DITEKCKQHY
110 120 130 140 150
GDALAGKIFS VRCKRAGKHA FSSMDVEKYV GSKLRRECGA AGIDLKAPQI
160 170 180 190 200
EVRIEVRDKR LFVIHSQHNG IGGYPLGALE QTLVLMSGGF DSTVAAYQIM
210 220 230 240 250
RRGLMAHFCF FNLGGRAHEL GVMEVAHFIW KKYGSSQRVL FVSVPFEEVL
260 270 280 290 300
GEILGKVDNS HMGVVLKRMM LRASSEIAER LHIDALVTGE AISQVSSQTL
310 320 330 340 350
PNLSVIDCVT DKLVLRPLIV AHKQDIIDTA NEIGTADFAR HMPEYCGVIS
360 370 380 390 400
VNPKTAAKRG RVEHEEKEFD MAVLERALAN AKLVPIDRVI DELGQDLQIE
410 420 430 440 450
EVSEALAGQI VIDIRHPDAA EDDPLELAGI EVQTMPFYAV NARFKELDPT
460 470 480
RQYLLYCDKG VMSRLHAHHL LSEGHANVRV YRPS
Length:484
Mass (Da):54,166
Last modified:April 12, 2017 - v1
Checksum:iA44FC824B7E6D731
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MQUK01000003 Genomic DNA Translation: OJT52104.1
RefSeqiWP_024011233.1, NZ_MQUK01000003.1

Genome annotation databases

GeneIDi32066507

Similar proteinsi

Entry informationi

Entry nameiA0A1Q3I9R3_9PSED
AccessioniPrimary (citable) accession number: A0A1Q3I9R3
Entry historyiIntegrated into UniProtKB/TrEMBL: April 12, 2017
Last sequence update: April 12, 2017
Last modified: March 28, 2018
This is version 9 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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