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Protein

Pyruvate carboxylase

Gene

cfiB_1

Organism
Mycobacteroides abscessus subsp. abscessus
Status
Unreviewed-Annotation score: -Protein predictedi

Functioni

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.UniRule annotation

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.UniRule annotation

Cofactori

biotinUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117ATPUniRule annotation1
Binding sitei200ATPUniRule annotation1
Binding sitei235ATPUniRule annotation1
Active sitei292UniRule annotation1
Metal bindingi533Divalent metal cationUniRule annotation1
Binding sitei605SubstrateUniRule annotation1
Metal bindingi703Divalent metal cation; via carbamate groupUniRule annotation1
Metal bindingi732Divalent metal cationUniRule annotation1
Metal bindingi734Divalent metal cationUniRule annotation1
Binding sitei867SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
LigandATP-bindingUniRule annotation, BiotinUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylaseUniRule annotation (EC:6.4.1.1UniRule annotation)
Gene namesi
Name:cfiB_1Imported
Synonyms:cfiBImported
ORF Names:SAMEA2068975_00952Imported, SAMEA2070424_04961Imported, SAMEA2070599_03374Imported, SAMEA2071298_04977Imported, SAMEA2071560_02589Imported, SAMEA2145025_00728Imported, SAMEA2275790_03061Imported, SAMEA2275861_03859Imported
OrganismiMycobacteroides abscessus subsp. abscessusImported
Taxonomic identifieri1185650 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteroidesMycobacteroides abscessus
Proteomesi
  • UP000248551 Componenti: Unassembled WGS sequence
  • UP000184814 Componenti: Unassembled WGS sequence
  • UP000249599 Componenti: Unassembled WGS sequence
  • UP000248755 Componenti: Unassembled WGS sequence
  • UP000184667 Componenti: Unassembled WGS sequence
  • UP000249750 Componenti: Unassembled WGS sequence
  • UP000249368 Componenti: Unassembled WGS sequence
  • UP000248947 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 449Biotin carboxylationInterPro annotationAdd BLAST449
Domaini121 – 317ATP-graspInterPro annotationAdd BLAST197
Domaini524 – 793Pyruvate carboxyltransferaseInterPro annotationAdd BLAST270
Domaini1053 – 1127Lipoyl-bindingInterPro annotationAdd BLAST75

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili174 – 194Sequence analysisAdd BLAST21

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR000891 PYR_CT
IPR005930 Pyruv_COase
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
PIRSFiPIRSF001594 Pyruv_carbox, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01235 pyruv_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00867 CPSASE_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1N2UKF5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSKVLVANR GEIAIRAFRA AYELGAETVA VYPYEDRNSG HRLKADEAYQ
60 70 80 90 100
IGEKGHPVRA YLSVSEVVNA ARNAGADAIY PGYGFLSENP DLAAACAEAG
110 120 130 140 150
ITFVGPPADV LELTGNKARA IAAAREAGLP VLASSEPSAS VEELLTAAES
160 170 180 190 200
MEFPVFVKAV AGGGGRGMRR VAERDQLREA IEAASREAES AFGDPTVFLE
210 220 230 240 250
QAVINPRHIE VQILADTAGN VIHLYERDCS VQRRHQKVIE LAPAPNLPPE
260 270 280 290 300
LREKICSDAV AFARHIGYSC AGTVEFLLDE RGHHVFIEMN PRIQVEHTVT
310 320 330 340 350
EEITDVDLVA SQLRIASGET LDDLGLHQDS ITPRGAALQC RITTEDPANG
360 370 380 390 400
FRPDTGRITA YRSPGGAGIR LDGGTTLGAE VGAHFDSMLV KLTCRGRDFQ
410 420 430 440 450
TAVARARRAV AEFRIRGVAT NIPFLQAVLD DPDFRAGHVT TSFIEERPQL
460 470 480 490 500
LTARSSADRG TKILNYLADV TVNKPHGAKP STVYPHDKLP PIDLSVPPPD
510 520 530 540 550
GSRQRLLALG PEGFAKALRE QKAVGLTDTT FRDAHQSLLA TRVRTTGLLA
560 570 580 590 600
VAPYVARLTP ELLSIEAWGG ATYDVALRFL KEDPWERLAD LREAVPNICL
610 620 630 640 650
QMLLRGRNTV GYTPYPETVT KAFIREASAT GVDIYRIFDA LNNIEAMRPA
660 670 680 690 700
IDAVREVGTS VAEVAMCYTG DLSNPGENLY TLDYYLKLAE QIVDAGAHVL
710 720 730 740 750
AIKDMAGLLR PPAAATLVTA LRSRFDLPVH VHTHDTAGGQ LATYLAAWQA
760 770 780 790 800
GADAVDGAAA PMAGTTSQPS LSAIVAAAAH SEYDTGVSLT SVCDLEPYWE
810 820 830 840 850
ALRKVYAPFE SGLPSPTGRV YTHEIPGGQL SNLRQQAIAL GLGDQFEEIE
860 870 880 890 900
ARYAAADRML GRLVKVTPSS KVVGDLALAL VGTGVSADEF ASDPGRFDIP
910 920 930 940 950
ESVIGFLRGE LGDPPGGWPE PFRTRALQGR GPARAEVALT ADDESQLDGD
960 970 980 990 1000
SATRRAALNR LLFPGPTKEF LAHREQYGDT SRLSANQFFY GLRYGEEHRV
1010 1020 1030 1040 1050
KLEKGVELLI GLEAISDADE HGMRTVMCIL NGQLRPVQVR DRSIESAVAS
1060 1070 1080 1090 1100
AEKADRANAD HVPAPFAGVV TLNVVSGQEV SAGETIGTIE AMKMEASITA
1110 1120
PKAGTVARVA LTETAQVEGG DLLVVIS
Length:1,127
Mass (Da):120,906
Last modified:March 15, 2017 - v1
Checksum:i339C748708B19D88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FSAD01000015 Genomic DNA Translation: SHR31256.1
FSAB01000015 Genomic DNA Translation: SHR32177.1
FSEE01000003 Genomic DNA Translation: SHV85757.1
FSGQ01000009 Genomic DNA Translation: SHZ64712.1
FSQV01000008 Genomic DNA Translation: SIN41640.1
FVKM01000006 Genomic DNA Translation: SKQ79847.1
FVPM01000006 Genomic DNA Translation: SKX43582.1
FVWC01000003 Genomic DNA Translation: SLF97602.1
RefSeqiWP_005087746.1, NZ_NQUY01000001.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FSAD01000015 Genomic DNA Translation: SHR31256.1
FSAB01000015 Genomic DNA Translation: SHR32177.1
FSEE01000003 Genomic DNA Translation: SHV85757.1
FSGQ01000009 Genomic DNA Translation: SHZ64712.1
FSQV01000008 Genomic DNA Translation: SIN41640.1
FVKM01000006 Genomic DNA Translation: SKQ79847.1
FVPM01000006 Genomic DNA Translation: SKX43582.1
FVWC01000003 Genomic DNA Translation: SLF97602.1
RefSeqiWP_005087746.1, NZ_NQUY01000001.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR000891 PYR_CT
IPR005930 Pyruv_COase
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
PIRSFiPIRSF001594 Pyruv_carbox, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01235 pyruv_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00867 CPSASE_2, 1 hit
PS50991 PYR_CT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A1N2UKF5_9MYCO
AccessioniPrimary (citable) accession number: A0A1N2UKF5
Entry historyiIntegrated into UniProtKB/TrEMBL: March 15, 2017
Last sequence update: March 15, 2017
Last modified: October 10, 2018
This is version 13 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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