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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Nitrosospira multiformis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Non-allosteric.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. Fructose-bisphosphate aldolase (SAMN05216412_10451), Fructose-bisphosphate aldolase (C8R21_1094), Fructose-bisphosphate aldolase (SAMN05216417_106135), Fructose-bisphosphate aldolase (SAMN05216404_10682), Fructose-1,6-bisphosphate aldolase (C8R20_102257), Fructose-1,6-bisphosphate aldolase (C8R21_101162)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13Diphosphate; via amide nitrogenUniRule annotation1
Sitei115Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation1
Sitei141Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation1
Active sitei144Proton acceptorUniRule annotation1
Binding sitei247SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, Transferase
Biological processGlycolysisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Short name:
PPi-PFKUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
ORF Names:SAMN05216404_107123Imported
OrganismiNitrosospira multiformisImported
Taxonomic identifieri1231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000183898 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 324PFKInterPro annotationAdd BLAST315

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni142 – 144Substrate bindingUniRule annotation3
Regioni190 – 192Substrate bindingUniRule annotation3
Regioni297 – 300Substrate bindingUniRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili83 – 103Sequence analysisAdd BLAST21

Sequence similaritiesi

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

HAMAPiMF_01978 Phosphofructokinase_II_B2, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR011404 PPi-PFK_XF0274
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF036483 PFK_XF0274, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1H8JN33-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAKNAFYAQ SGGVTAVINA SAAGVIETAR KQSGKIGRIY AGRNGIIGAL
60 70 80 90 100
TEDLIDTGQE SDAAISALRY TPSGAFGSCR YKLKSLEQNR REYERLIEVF
110 120 130 140 150
KAHDIGYFFY NGGGDSADTC LKVSQLSGTL GYPIQAIHVP KTVDNDLPIT
160 170 180 190 200
DCCPGFGSVA KYIAVSTLEA SFDVASMSAT STKVFVLEVM GRHAGWIAAA
210 220 230 240 250
GGLASSPERE IPVVILFPEI SFDKQKFLAK VDSCVKKFGY CSVVVSEGVK
260 270 280 290 300
GDDGKFLSDQ GVRDAFGHAQ LGGVAPVVAN MVKEGLGLKY HWGVADYLQR
310 320 330 340 350
AARHIASKTD VEQAYAMGQA AVEFAVQGHN SVMPTIERIS ARPYQWKVGM
360 370 380 390 400
AQLSQVANVE KMMPENFITE DGFGITDQCR EYLAPLIEGE DYPPYRDGLP
410
DYVRLKNVAV AKKLGGFTL
Length:419
Mass (Da):45,106
Last modified:April 12, 2017 - v1
Checksum:i4380198B0C202CF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FOCT01000007 Genomic DNA Translation: SEN81961.1
RefSeqiWP_074746694.1, NZ_FOCT01000007.1

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FOCT01000007 Genomic DNA Translation: SEN81961.1
RefSeqiWP_074746694.1, NZ_FOCT01000007.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Family and domain databases

HAMAPiMF_01978 Phosphofructokinase_II_B2, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR011404 PPi-PFK_XF0274
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF036483 PFK_XF0274, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A1H8JN33_9PROT
AccessioniPrimary (citable) accession number: A0A1H8JN33
Entry historyiIntegrated into UniProtKB/TrEMBL: April 12, 2017
Last sequence update: April 12, 2017
Last modified: September 12, 2018
This is version 7 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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