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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Fervidobacterium thailandense
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei549Aminoacyl-adenylateUniRule annotation1
Binding sitei593ATPUniRule annotation1
Metal bindingi882ZincUniRule annotation1
Metal bindingi885ZincUniRule annotation1
Metal bindingi902ZincUniRule annotation1
Metal bindingi905ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
ORF Names:A4H02_08160Imported
OrganismiFervidobacterium thailandenseImported
Taxonomic identifieri1008305 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeFervidobacterium
Proteomesi
  • UP000094570 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 627tRNA-synt_1InterPro annotationAdd BLAST601
Domaini672 – 827Anticodon_1InterPro annotationAdd BLAST156
Domaini879 – 906zf-FPG_IleRSInterPro annotationAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionUniRule annotationAdd BLAST11
Motifi590 – 594"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationSAAS annotation

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1E3G0X4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDYKETLNLP RTNFQMKANL VNKEPEILKR WEEQKIYEKT LEYRADAPTY
60 70 80 90 100
LLHDGPPYAN GDIHLGTAMN KVLKDFVTRY KTMRGYRVPY VPGWDTHGLP
110 120 130 140 150
IEHRVTTMLG DEAKNKTPVE IRRLCKDFAL KFVNVQREQF KRLGVKGDWD
160 170 180 190 200
NPYITLDPEY EYHILDVFKT LVETGNVYRG NKPVYWCPSC KTALAEAEIE
210 220 230 240 250
YHDHESPSIY VKFQMVDDPK TYIVIWTTTP WTIPANVAIA LHPEYTYTKI
260 270 280 290 300
KVGEEYWIVA EGLLQKFSAE TGVHFEVVEK FLGSELEGKL TKHPLYDRTS
310 320 330 340 350
VVVLADYVTL EDGTGCVHTA PGHGEEDYQT GLKYNLPVLS PVDDEGRFTK
360 370 380 390 400
EAGKYEGLKI WDANKIIVED LKNNGALIKY GKIQHSYPHC WRCKGPVIFR
410 420 430 440 450
ATPQWFISVD KNDLRKKVLE QIRNVKWYPA WGENRITAMV QERPDWTISR
460 470 480 490 500
QRVWGVPIPA VVCRQCGEVI LDAKVIEKFA NIVREKGTDA WFEMDVENFI
510 520 530 540 550
PEGFTCSKCG GSEFDKTYDT LDVWIDSGCS WEAVIRSKGE KFPVDLYLEG
560 570 580 590 600
DDQHRGWFQS SIFLSTAKTG VAPFRAVVTH GFIKDEQGRK MSKSLGNVVD
610 620 630 640 650
PMEIVNKYGA DILRLWVAST DFFDNIRVGK NIIEQQVEVY RKLRNTIRYL
660 670 680 690 700
LSNLYDFTEA DLVPYEKLLP LDKWALGRLQ KYIEQVTSYY EEFEYSKVYN
710 720 730 740 750
ATVKYCTTEL SAVYLDILKD RLYVEAKDSI YRRSAQTVLY YILDALIKIL
760 770 780 790 800
APIIPFTAEE AYQESPAKRF ESVHLEYWPE VRKEFIDEQL LEEFQHLLLV
810 820 830 840 850
RDDVLKALEE ARASDLIGHS LDAHVYIEPK NEEIAALLKK YEGILDEFFI
860 870 880 890 900
VSAVTLTDEN LEGAVTGQFV DVLVKHAEGE KCQRCWKYHP DTGKDESHPE
910
TCPRCSAVLR GERR
Length:914
Mass (Da):105,698
Last modified:January 18, 2017 - v1
Checksum:i600A91DDB017621D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LWAF01000015 Genomic DNA Translation: ODN29896.1
RefSeqiWP_069293691.1, NZ_LWAF01000015.1

Genome annotation databases

EnsemblBacteriaiODN29896; ODN29896; A4H02_08160

Similar proteinsi

Entry informationi

Entry nameiA0A1E3G0X4_9BACT
AccessioniPrimary (citable) accession number: A0A1E3G0X4
Entry historyiIntegrated into UniProtKB/TrEMBL: January 18, 2017
Last sequence update: January 18, 2017
Last modified: March 28, 2018
This is version 13 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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