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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Clostridium pasteurianum
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotationSAAS annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi129Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotation
Biological processNitrogen fixationUniRule annotationSAAS annotation
Ligand4Fe-4SUniRule annotationSAAS annotation, ATP-bindingUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron proteinUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
Nitrogenase Fe proteinUniRule annotation
Nitrogenase component IIUniRule annotation
Nitrogenase reductaseUniRule annotation
Gene namesi
Name:nifHUniRule annotationImported
ORF Names:AQ984_06920Imported
OrganismiClostridium pasteurianumImported
Taxonomic identifieri1501 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000178420 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseUniRule annotation1

Post-translational modificationi

The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation
The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation

Keywords - PTMi

ADP-ribosylationUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

SMRiA0A1D9N1X8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd02040 NifH, 1 hit
HAMAPiMF_00533 NifH, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR005977 Nitrogenase_Fe_NifH
IPR027417 P-loop_NTPase
PANTHERiPTHR42864 PTHR42864, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01287 nifH, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1D9N1X8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRQVAIYGKG GIGKSTTTQN LTSGLHAMGK TIMVVGCDPK ADSTRLLLGG
60 70 80 90 100
LAQKSVLDTL REEGEDVELD SILKEGYGGI RCVESGGPEP GVGCAGRGII
110 120 130 140 150
TSINMLEQLG AYTDDLDYVF YDVLGDVVCG GFAMPIREGK AQEIYIVASG
160 170 180 190 200
EMMALYAANN ISKGIQKYAK SGGVRLGGII CNSRKVANEY ELLDAFAKEL
210 220 230 240 250
GSQLIHFVPR SPMVTKAEIN KQTVIEYDPT CEQAEEYREL ARKVDANELF
260 270
VIPKPMTQER LEEILMQYGL MDL
Length:273
Mass (Da):29,662
Last modified:February 15, 2017 - v1
Checksum:iA4EC06C5F58F08EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP013019 Genomic DNA Translation: AOZ78629.1
RefSeqiWP_003447877.1, NZ_CP013019.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP013019 Genomic DNA Translation: AOZ78629.1
RefSeqiWP_003447877.1, NZ_CP013019.1

3D structure databases

SMRiA0A1D9N1X8
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd02040 NifH, 1 hit
HAMAPiMF_00533 NifH, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR005977 Nitrogenase_Fe_NifH
IPR027417 P-loop_NTPase
PANTHERiPTHR42864 PTHR42864, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01287 nifH, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A1D9N1X8_CLOPA
AccessioniPrimary (citable) accession number: A0A1D9N1X8
Entry historyiIntegrated into UniProtKB/TrEMBL: February 15, 2017
Last sequence update: February 15, 2017
Last modified: October 10, 2018
This is version 10 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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