Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 28 (07 Apr 2021)
Sequence version 2 (18 Sep 2019)
Previous versions | rss
Add a publicationFeedback
Protein

Ent-copalyl diphosphate synthase 2, chloroplastic

Gene

CPPS2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in gibberellin biosynthesis (PubMed:16307364).

Catalyzes the conversion of geranylgeranyl diphosphate to the gibberellin precursor ent-copalyl diphosphate (ent-CPP) (PubMed:16307364).

Involved in the production of antifungal dolabralexin phytoalexins in response to biotic and abiotic stresses (PubMed:29475898).

In response to fungal infection and in associtation with KSL4, is involved in the production dolabradiene, a type of antifungal phytoalexin (PubMed:29475898).

2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: gibberellin biosynthesis

This protein is involved in the pathway gibberellin biosynthesis, which is part of Plant hormone biosynthesis.Curated
View all proteins of this organism that are known to be involved in the pathway gibberellin biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei241SubstrateBy similarity1
Binding sitei459SubstrateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIsomerase
Biological processPlant defense
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00390

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ent-copalyl diphosphate synthase 2, chloroplasticCurated (EC:5.5.1.132 Publications)
Short name:
Ent-CPP synthase 21 Publication
Short name:
Ent-CPS 21 Publication
Alternative name(s):
Protein ANTHER EAR 2Curated
Short name:
ZmAN21 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CPPS2Curated
Synonyms:AN21 Publication
ORF Names:ZEAMMB73_Zm00001d029648Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiZea mays (Maize)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri4577 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000007305 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

Maize Genetics and Genomics Database

More...
MaizeGDBi
85297

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Chloroplast, Plastid

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 70ChloroplastSequence analysisAdd BLAST70
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000044777071 – 825Ent-copalyl diphosphate synthase 2, chloroplasticAdd BLAST755

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in tassels.1 Publication

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Induced by the fungal pathogens Fusarium graminearum and Fusarium verticillioides.1 Publication

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
A0A1D6K6U5, baseline and differential

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
4577.GRMZM2G044481_P01

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
A0A1D6K6U5

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi373 – 376DXDD motifCurated4

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity through binding to Mg2+.Curated

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the terpene synthase family. Tpsc subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG502QQN6, Eukaryota

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.600.10, 1 hit
1.50.10.130, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008949, Isoprenoid_synthase_dom_sf
IPR001906, Terpene_synth_N
IPR036965, Terpene_synth_N_sf
IPR005630, Terpene_synthase_metal-bd
IPR008930, Terpenoid_cyclase/PrenylTrfase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01397, Terpene_synth, 1 hit
PF03936, Terpene_synth_C, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48239, SSF48239, 2 hits
SSF48576, SSF48576, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

A0A1D6K6U5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLSSSCTTV PHLSSLAVVQ LGPWSSRIKK KTDAVAVPAA AGRWRARARA
60 70 80 90 100
QDTSESAAVA KGSSLTPIVR TDAESRRTRW PTDDDDAEPL VDEIRAMLTS
110 120 130 140 150
MSDGDISVSA YDTAWVGLVP RLDGGEGPQF PAAVRWIRNN QLPDGSWGDA
160 170 180 190 200
ALFSAYDRLI NTLACVVTLT RWSLEPEMRG RGLSFLGRNM WKLATEDEES
210 220 230 240 250
MPIGFELAFP SLIELAKSLG VHDFPYDHQA LQAIYSSREI KVKRIPKEVM
260 270 280 290 300
HTVPTSILHS LEGMPGLDWA RLLKLQSSDG SFLFSPAATA YALMNTGDDR
310 320 330 340 350
CFSYIDRTVK KFNGGVPNVY PVDLFEHIWA VDRLERLGIS RYFQKEIEQC
360 370 380 390 400
MDYVNRHWTE DGICWARNSD VKEVDDTAMA FRLLRLHGYS VSPDVFKNFE
410 420 430 440 450
KDGEFFAFVG QSNQAVTGMY NLNRASQISF PGEDVLHRAG PFSYEFLRRK
460 470 480 490 500
QAEGALRDKW IISKDLPGEV VYTLDFPWYG NLPRVEARDY LEQYGGGDDV
510 520 530 540 550
WIGKTLYRMP LVNNDVYLEL ARMDFNHCQA LHQLEWQGLK KWYTENRLMD
560 570 580 590 600
FGVAQEDALR AYFLAAASVY EPCRAAERLA WARAAILANA VSTHLRNSPS
610 620 630 640 650
FRERLEHSLR CRPSEETDGS WFNSSSGSDA VLVKAVLRLT DSLAREAQPI
660 670 680 690 700
HGGDPEDIHK LLRSAWAEWV REKADAADSV CNGSSAVEQE GSRMVHDKQT
710 720 730 740 750
CLLLARMIEI SAGRAAGEAA SEDGDRRIIQ LTGSICDSLK QKMLVSQDPE
760 770 780 790 800
KNEEMMSHVD DELKLRIREF VQYLLRLGEK KTGSSETRQT FLSIVKSCYY
810 820
AAHCPPHVVD RHISRVIFEP VSAAK
Length:825
Mass (Da):92,762
Last modified:September 18, 2019 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1004925ED2C9A152
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti34A → T in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti34A → T in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti45R → RR in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti45R → RR in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti47R → L in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti47R → L in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti52D → H in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti52D → H in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti233A → G in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti233A → G in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti242V → M in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti242V → M in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti271R → K in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti271R → K in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti441P → A in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti441P → A in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti451Q → E in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti451Q → E in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti541K → R in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti541K → R in AAT70084 (PubMed:16307364).Curated1
Sequence conflicti657D → DI in AAT70083 (PubMed:16307364).Curated1
Sequence conflicti657D → DI in AAT70084 (PubMed:16307364).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY562490 Genomic DNA Translation: AAT70083.1
AY562491 mRNA Translation: AAT70084.1
CM007647 Genomic DNA Translation: ONL99283.1

NCBI Reference Sequences

More...
RefSeqi
NP_001105257.2, NM_001111787.2

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
542165

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
zma:542165

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY562490 Genomic DNA Translation: AAT70083.1
AY562491 mRNA Translation: AAT70084.1
CM007647 Genomic DNA Translation: ONL99283.1
RefSeqiNP_001105257.2, NM_001111787.2

3D structure databases

SMRiA0A1D6K6U5
ModBaseiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G044481_P01

Genome annotation databases

GeneIDi542165
KEGGizma:542165

Organism-specific databases

MaizeGDBi85297

Phylogenomic databases

eggNOGiENOG502QQN6, Eukaryota

Enzyme and pathway databases

UniPathwayiUPA00390

Gene expression databases

ExpressionAtlasiA0A1D6K6U5, baseline and differential

Family and domain databases

Gene3Di1.10.600.10, 1 hit
1.50.10.130, 1 hit
InterProiView protein in InterPro
IPR008949, Isoprenoid_synthase_dom_sf
IPR001906, Terpene_synth_N
IPR036965, Terpene_synth_N_sf
IPR005630, Terpene_synthase_metal-bd
IPR008930, Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF01397, Terpene_synth, 1 hit
PF03936, Terpene_synth_C, 1 hit
SUPFAMiSSF48239, SSF48239, 2 hits
SSF48576, SSF48576, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCPPS2_MAIZE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A1D6K6U5
Secondary accession number(s): Q672R4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2019
Last sequence update: September 18, 2019
Last modified: April 7, 2021
This is version 28 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again