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Protein

Biotin synthase

Gene

bioB

Organism
Campylobacter ornithocola
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi20Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi23Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi60Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi153Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processBiotin biosynthesisUniRule annotationSAAS annotation
Ligand2Fe-2SUniRule annotationSAAS annotation, 4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotationSAAS annotation (EC:2.8.1.6UniRule annotationSAAS annotation)
Gene namesi
Name:bioBUniRule annotation
ORF Names:A7X81_01760Imported
OrganismiCampylobacter ornithocolaImported
Taxonomic identifieri1848766 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000094873 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 211Elp3InterPro annotationAdd BLAST206
Domaini183 – 273BATSInterPro annotationAdd BLAST91

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1C2FVS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIMLCAISN IASGGCGEDC KYCTQSAHVK TNINKYKRKD IDQIVLEAKM
60 70 80 90 100
AKKNEALGFC LVTAGAGLDD EKLEYVCKVA HAVQKEVEGL LLIACNGIAN
110 120 130 140 150
LEQLKELKKA GIFSYNHNLE TSKEFFPNIC STHTWEQRFQ TNLYAKEAGL
160 170 180 190 200
MLCCGGIYGL GESEVDRKSF RASLKELEPF SSPINFFIPN ENLKLKQALL
210 220 230 240 250
DPDEALNIIK DTKKDLPNAH IMVAGGREVV LKERQYEIFD AGASAIVVGD
260 270
YLTTKGEEPN KDIQKLKQMG FSFANSCH
Length:278
Mass (Da):30,855
Last modified:November 2, 2016 - v1
Checksum:iB558D1B04673473E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LXSU01000072 Genomic DNA Translation: OCX43389.1
RefSeqiWP_066006975.1, NZ_LXSU01000072.1

Genome annotation databases

EnsemblBacteriaiOCX43389; OCX43389; A7X81_01760

Similar proteinsi

Entry informationi

Entry nameiA0A1C2FVS5_9PROT
AccessioniPrimary (citable) accession number: A0A1C2FVS5
Entry historyiIntegrated into UniProtKB/TrEMBL: November 2, 2016
Last sequence update: November 2, 2016
Last modified: November 22, 2017
This is version 10 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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